Hydrolysis efficiency and enzyme adsorption on steam pretreated spruce in the presence of poly(ethylene glycol)

Bálint Sipos; Dóra Dienes; Áron Schleicher; Raffaella Perazzini; Claudia Crestini; Matti Siika-aho; Kati Réczey

doi:10.1016/j.enzmictec.2010.05.010

Hydrolysis efficiency and enzyme adsorption on steam pretreated spruce in the presence of poly(ethylene glycol)

Bálint Sipos (Corresponding Author), Dóra Dienes, Áron Schleicher, Raffaella Perazzini, Claudia Crestini, Matti Siika-aho, Kati Réczey

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

Enzymatic hydrolysis of lignocellulosic substrates is one of the limiting steps in second generation bio-ethanol production. The effects of addition of poly(ethylene glycol) 4000 (PEG) on the efficiency and adsorption of cellulase components during the 72-h hydrolysis of steam-pretreated spruce were investigated. It was found that addition of PEG increased the amount of the enzyme components in the hydrolysis supernatant to varying degrees. As an effect of PEG addition the concentration of free proteins increased by 36% and the free overall cellulolytic activity (FPA) in the liquid fraction by 51%. After 72-h hydrolysis with PEG 48–68% higher activities towards 4-methylumbelliferyl-saccharide substrates were recovered. The results suggest that the effect of PEG is based on the decrease of non-productive enzyme binding. The structure of the hydrolysis residues was studied by 31P NMR.

Original language	English
Pages (from-to)	84-90
Number of pages	7
Journal	Enzyme and Microbial Technology
Volume	47
Issue number	3
DOIs	https://doi.org/10.1016/j.enzmictec.2010.05.010
Publication status	Published - 2010
MoE publication type	A1 Journal article-refereed

Keywords

lignocellulose
cellulase
hydrolysis
enzyme adsorption
poly(ethylene glycol)

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10.1016/j.enzmictec.2010.05.010

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title = "Hydrolysis efficiency and enzyme adsorption on steam pretreated spruce in the presence of poly(ethylene glycol)",

abstract = "Enzymatic hydrolysis of lignocellulosic substrates is one of the limiting steps in second generation bio-ethanol production. The effects of addition of poly(ethylene glycol) 4000 (PEG) on the efficiency and adsorption of cellulase components during the 72-h hydrolysis of steam-pretreated spruce were investigated. It was found that addition of PEG increased the amount of the enzyme components in the hydrolysis supernatant to varying degrees. As an effect of PEG addition the concentration of free proteins increased by 36% and the free overall cellulolytic activity (FPA) in the liquid fraction by 51%. After 72-h hydrolysis with PEG 48–68% higher activities towards 4-methylumbelliferyl-saccharide substrates were recovered. The results suggest that the effect of PEG is based on the decrease of non-productive enzyme binding. The structure of the hydrolysis residues was studied by 31P NMR.",

keywords = "lignocellulose, cellulase, hydrolysis, enzyme adsorption, poly(ethylene glycol)",

author = "B{\'a}lint Sipos and D{\'o}ra Dienes and {\'A}ron Schleicher and Raffaella Perazzini and Claudia Crestini and Matti Siika-aho and Kati R{\'e}czey",

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doi = "10.1016/j.enzmictec.2010.05.010",

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volume = "47",

pages = "84--90",

journal = "Enzyme and Microbial Technology",

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TY - JOUR

T1 - Hydrolysis efficiency and enzyme adsorption on steam pretreated spruce in the presence of poly(ethylene glycol)

AU - Sipos, Bálint

AU - Dienes, Dóra

AU - Schleicher, Áron

AU - Perazzini, Raffaella

AU - Crestini, Claudia

AU - Siika-aho, Matti

AU - Réczey, Kati

PY - 2010

Y1 - 2010

N2 - Enzymatic hydrolysis of lignocellulosic substrates is one of the limiting steps in second generation bio-ethanol production. The effects of addition of poly(ethylene glycol) 4000 (PEG) on the efficiency and adsorption of cellulase components during the 72-h hydrolysis of steam-pretreated spruce were investigated. It was found that addition of PEG increased the amount of the enzyme components in the hydrolysis supernatant to varying degrees. As an effect of PEG addition the concentration of free proteins increased by 36% and the free overall cellulolytic activity (FPA) in the liquid fraction by 51%. After 72-h hydrolysis with PEG 48–68% higher activities towards 4-methylumbelliferyl-saccharide substrates were recovered. The results suggest that the effect of PEG is based on the decrease of non-productive enzyme binding. The structure of the hydrolysis residues was studied by 31P NMR.

AB - Enzymatic hydrolysis of lignocellulosic substrates is one of the limiting steps in second generation bio-ethanol production. The effects of addition of poly(ethylene glycol) 4000 (PEG) on the efficiency and adsorption of cellulase components during the 72-h hydrolysis of steam-pretreated spruce were investigated. It was found that addition of PEG increased the amount of the enzyme components in the hydrolysis supernatant to varying degrees. As an effect of PEG addition the concentration of free proteins increased by 36% and the free overall cellulolytic activity (FPA) in the liquid fraction by 51%. After 72-h hydrolysis with PEG 48–68% higher activities towards 4-methylumbelliferyl-saccharide substrates were recovered. The results suggest that the effect of PEG is based on the decrease of non-productive enzyme binding. The structure of the hydrolysis residues was studied by 31P NMR.

KW - lignocellulose

KW - cellulase

KW - hydrolysis

KW - enzyme adsorption

KW - poly(ethylene glycol)

U2 - 10.1016/j.enzmictec.2010.05.010

DO - 10.1016/j.enzmictec.2010.05.010

M3 - Article

SN - 0141-0229

VL - 47

SP - 84

EP - 90

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

IS - 3

ER -

Hydrolysis efficiency and enzyme adsorption on steam pretreated spruce in the presence of poly(ethylene glycol)

Abstract

Keywords

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