Hydrolysis of barley (1->3), (1->4)-b-D-glucan by a cellobiohydrolase II preparation from Trichoderma reesei

Kirsti Henriksson, Anita Teleman, Tapani Suortti, Tapani Reinikainen, Johanna Jaskari, Olle Teleman (Corresponding Author), Kaisa Poutanen

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Abstract

The molecular weight of commercial barley β-glucan was 250,000 as determined by dual angle laser light scattering. 1H NMR analysis showed the polymer to contain 29% (1→3)-linkages and 71% (1→4)-linkages.
The barley β-glucan was readily hydrolysed by a highly purified cellobiohydrolase II (CBHII) preparation from Trichoderma reesei. NMR data demonstrated that the cellulase preparation degraded only (1→4)-linkages in the β-glucan chain. Neither internal G(1→3)G(1→4)G nor reducing end G(1→3)G(1→4)G(1→4)G sequences were hydrolysed.
The main hydrolysis products were: cellobiose, β-d-Glcp-(1→3)-β-d-Glcp-(l→4)-d-Glcp, β-d-Glcp-(1→3)-β-d-Glcp-(1→4)-β-d-Glcp-(1→4)-d-Glcp and β-d-Glcp-(1→4)-β-d-Glcd-(1→3)-β-d-Glcp-(1→4)-β-d-Glcp.
Statistical models of the glucan linkage sequence were fitted to the relative fragment concentrations after CBHII and lichenase degradations.
The hydrolysate compositions are well reproduced by a second order Markov chain.
All degradation data are consistent with the assumed degradation mechanisms of the two enzymes, including the hypothesis that hydrolysis by CBHII depends on the glycosidic bond orientation.

Original languageEnglish
Pages (from-to)109-119
JournalCarbohydrate Polymers
Volume26
Issue number2
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

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Cellulose 1,4-beta-Cellobiosidase
Glucans
Hydrolysis
Degradation
Nuclear magnetic resonance
Cellobiose
Light scattering
Markov processes
Cellulase
Enzymes
Molecular weight
Polymers
Lasers
Chemical analysis
polyglucosan

Cite this

Henriksson, Kirsti ; Teleman, Anita ; Suortti, Tapani ; Reinikainen, Tapani ; Jaskari, Johanna ; Teleman, Olle ; Poutanen, Kaisa. / Hydrolysis of barley (1->3), (1->4)-b-D-glucan by a cellobiohydrolase II preparation from Trichoderma reesei. In: Carbohydrate Polymers. 1995 ; Vol. 26, No. 2. pp. 109-119.
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title = "Hydrolysis of barley (1->3), (1->4)-b-D-glucan by a cellobiohydrolase II preparation from Trichoderma reesei",
abstract = "The molecular weight of commercial barley β-glucan was 250,000 as determined by dual angle laser light scattering. 1H NMR analysis showed the polymer to contain 29{\%} (1→3)-linkages and 71{\%} (1→4)-linkages. The barley β-glucan was readily hydrolysed by a highly purified cellobiohydrolase II (CBHII) preparation from Trichoderma reesei. NMR data demonstrated that the cellulase preparation degraded only (1→4)-linkages in the β-glucan chain. Neither internal G(1→3)G(1→4)G nor reducing end G(1→3)G(1→4)G(1→4)G sequences were hydrolysed. The main hydrolysis products were: cellobiose, β-d-Glcp-(1→3)-β-d-Glcp-(l→4)-d-Glcp, β-d-Glcp-(1→3)-β-d-Glcp-(1→4)-β-d-Glcp-(1→4)-d-Glcp and β-d-Glcp-(1→4)-β-d-Glcd-(1→3)-β-d-Glcp-(1→4)-β-d-Glcp. Statistical models of the glucan linkage sequence were fitted to the relative fragment concentrations after CBHII and lichenase degradations. The hydrolysate compositions are well reproduced by a second order Markov chain. All degradation data are consistent with the assumed degradation mechanisms of the two enzymes, including the hypothesis that hydrolysis by CBHII depends on the glycosidic bond orientation.",
author = "Kirsti Henriksson and Anita Teleman and Tapani Suortti and Tapani Reinikainen and Johanna Jaskari and Olle Teleman and Kaisa Poutanen",
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year = "1995",
doi = "10.1016/0144-8617(94)00103-Z",
language = "English",
volume = "26",
pages = "109--119",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
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Hydrolysis of barley (1->3), (1->4)-b-D-glucan by a cellobiohydrolase II preparation from Trichoderma reesei. / Henriksson, Kirsti; Teleman, Anita; Suortti, Tapani; Reinikainen, Tapani; Jaskari, Johanna; Teleman, Olle (Corresponding Author); Poutanen, Kaisa.

In: Carbohydrate Polymers, Vol. 26, No. 2, 1995, p. 109-119.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Hydrolysis of barley (1->3), (1->4)-b-D-glucan by a cellobiohydrolase II preparation from Trichoderma reesei

AU - Henriksson, Kirsti

AU - Teleman, Anita

AU - Suortti, Tapani

AU - Reinikainen, Tapani

AU - Jaskari, Johanna

AU - Teleman, Olle

AU - Poutanen, Kaisa

N1 - Project code: KET4110

PY - 1995

Y1 - 1995

N2 - The molecular weight of commercial barley β-glucan was 250,000 as determined by dual angle laser light scattering. 1H NMR analysis showed the polymer to contain 29% (1→3)-linkages and 71% (1→4)-linkages. The barley β-glucan was readily hydrolysed by a highly purified cellobiohydrolase II (CBHII) preparation from Trichoderma reesei. NMR data demonstrated that the cellulase preparation degraded only (1→4)-linkages in the β-glucan chain. Neither internal G(1→3)G(1→4)G nor reducing end G(1→3)G(1→4)G(1→4)G sequences were hydrolysed. The main hydrolysis products were: cellobiose, β-d-Glcp-(1→3)-β-d-Glcp-(l→4)-d-Glcp, β-d-Glcp-(1→3)-β-d-Glcp-(1→4)-β-d-Glcp-(1→4)-d-Glcp and β-d-Glcp-(1→4)-β-d-Glcd-(1→3)-β-d-Glcp-(1→4)-β-d-Glcp. Statistical models of the glucan linkage sequence were fitted to the relative fragment concentrations after CBHII and lichenase degradations. The hydrolysate compositions are well reproduced by a second order Markov chain. All degradation data are consistent with the assumed degradation mechanisms of the two enzymes, including the hypothesis that hydrolysis by CBHII depends on the glycosidic bond orientation.

AB - The molecular weight of commercial barley β-glucan was 250,000 as determined by dual angle laser light scattering. 1H NMR analysis showed the polymer to contain 29% (1→3)-linkages and 71% (1→4)-linkages. The barley β-glucan was readily hydrolysed by a highly purified cellobiohydrolase II (CBHII) preparation from Trichoderma reesei. NMR data demonstrated that the cellulase preparation degraded only (1→4)-linkages in the β-glucan chain. Neither internal G(1→3)G(1→4)G nor reducing end G(1→3)G(1→4)G(1→4)G sequences were hydrolysed. The main hydrolysis products were: cellobiose, β-d-Glcp-(1→3)-β-d-Glcp-(l→4)-d-Glcp, β-d-Glcp-(1→3)-β-d-Glcp-(1→4)-β-d-Glcp-(1→4)-d-Glcp and β-d-Glcp-(1→4)-β-d-Glcd-(1→3)-β-d-Glcp-(1→4)-β-d-Glcp. Statistical models of the glucan linkage sequence were fitted to the relative fragment concentrations after CBHII and lichenase degradations. The hydrolysate compositions are well reproduced by a second order Markov chain. All degradation data are consistent with the assumed degradation mechanisms of the two enzymes, including the hypothesis that hydrolysis by CBHII depends on the glycosidic bond orientation.

U2 - 10.1016/0144-8617(94)00103-Z

DO - 10.1016/0144-8617(94)00103-Z

M3 - Article

VL - 26

SP - 109

EP - 119

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

IS - 2

ER -