Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC

Anita Teleman; Anne Valkeajärvi; Anu Koivula; Laura Ruohonen; Torbjörn Drakenberg

doi:10.1533/9781845698539.1.37

Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC

Anita Teleman, Anne Valkeajärvi, Anu Koivula, Laura Ruohonen, Torbjörn Drakenberg

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

Abstract

Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min−1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.8-21 min−1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.

Original language	English
Title of host publication	Cellulose and Cellulose Derivatives
Subtitle of host publication	Physicochemical Aspects and Industrial Applications
Editors	John F. Kennedy, Glyn O. Phillips, Peter A. Williams, Lennart Piculell
Place of Publication	Cambridge
Publisher	Woodhead Publishing
Pages	37-42
ISBN (Print)	978-1-85573-212-4
DOIs	https://doi.org/10.1533/9781845698539.1.37
Publication status	Published - 1995
MoE publication type	A4 Article in a conference publication
Event	Cellucon '93 - Lund, Sweden Duration: 21 Jun 1993 → 24 Jun 1993

Conference

Conference	Cellucon '93
Country/Territory	Sweden
City	Lund
Period	21/06/93 → 24/06/93

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10.1533/9781845698539.1.37

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Teleman, A., Valkeajärvi, A., Koivula, A., Ruohonen, L., & Drakenberg, T. (1995). Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC. In J. F. Kennedy, G. O. Phillips, P. A. Williams, & L. Piculell (Eds.), Cellulose and Cellulose Derivatives: Physicochemical Aspects and Industrial Applications (pp. 37-42). Woodhead Publishing. https://doi.org/10.1533/9781845698539.1.37

Teleman, Anita ; Valkeajärvi, Anne ; Koivula, Anu et al. / Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC. Cellulose and Cellulose Derivatives: Physicochemical Aspects and Industrial Applications. editor / John F. Kennedy ; Glyn O. Phillips ; Peter A. Williams ; Lennart Piculell. Cambridge : Woodhead Publishing, 1995. pp. 37-42

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title = "Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC",

abstract = "Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min−1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.8-21 min−1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.",

author = "Anita Teleman and Anne Valkeaj{\"a}rvi and Anu Koivula and Laura Ruohonen and Torbj{\"o}rn Drakenberg",

note = "Project code: KET3112 Project code: BIO2024; Cellucon '93 ; Conference date: 21-06-1993 Through 24-06-1993",

year = "1995",

doi = "10.1533/9781845698539.1.37",

language = "English",

isbn = "978-1-85573-212-4",

pages = "37--42",

editor = "Kennedy, {John F.} and Phillips, {Glyn O.} and Williams, {Peter A.} and Lennart Piculell",

booktitle = "Cellulose and Cellulose Derivatives",

publisher = "Woodhead Publishing",

address = "United Kingdom",

}

Teleman, A, Valkeajärvi, A, Koivula, A, Ruohonen, L & Drakenberg, T 1995, Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC. in JF Kennedy, GO Phillips, PA Williams & L Piculell (eds), Cellulose and Cellulose Derivatives: Physicochemical Aspects and Industrial Applications. Woodhead Publishing, Cambridge, pp. 37-42, Cellucon '93, Lund, Sweden, 21/06/93. https://doi.org/10.1533/9781845698539.1.37

Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC. / Teleman, Anita; Valkeajärvi, Anne; Koivula, Anu et al.
Cellulose and Cellulose Derivatives: Physicochemical Aspects and Industrial Applications. ed. / John F. Kennedy; Glyn O. Phillips; Peter A. Williams; Lennart Piculell. Cambridge: Woodhead Publishing, 1995. p. 37-42.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

TY - GEN

T1 - Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC

AU - Teleman, Anita

AU - Valkeajärvi, Anne

AU - Koivula, Anu

AU - Ruohonen, Laura

AU - Drakenberg, Torbjörn

N1 - Project code: KET3112 Project code: BIO2024

PY - 1995

Y1 - 1995

N2 - Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min−1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.8-21 min−1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.

AB - Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min−1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.8-21 min−1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.

U2 - 10.1533/9781845698539.1.37

DO - 10.1533/9781845698539.1.37

M3 - Conference article in proceedings

SN - 978-1-85573-212-4

SP - 37

EP - 42

BT - Cellulose and Cellulose Derivatives

A2 - Kennedy, John F.

A2 - Phillips, Glyn O.

A2 - Williams, Peter A.

A2 - Piculell, Lennart

PB - Woodhead Publishing

CY - Cambridge

T2 - Cellucon '93

Y2 - 21 June 1993 through 24 June 1993

ER -

Teleman A, Valkeajärvi A, Koivula A, Ruohonen L, Drakenberg T. Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC. In Kennedy JF, Phillips GO, Williams PA, Piculell L, editors, Cellulose and Cellulose Derivatives: Physicochemical Aspects and Industrial Applications. Cambridge: Woodhead Publishing. 1995. p. 37-42 doi: 10.1533/9781845698539.1.37

Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC

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