Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC

Anita Teleman, Anne Valkeajärvi, Anu Koivula, Laura Ruohonen, Torbjörn Drakenberg

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min−1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.8-21 min−1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.
Original languageEnglish
Title of host publicationCellulose and Cellulose Derivatives
Subtitle of host publicationPhysicochemical Aspects and Industrial Applications
EditorsJohn F. Kennedy, Glyn O. Phillips, Peter A. Williams, Lennart Piculell
Place of PublicationCambridge
PublisherWoodhead Publishing
Pages37-42
ISBN (Print)978-1-85573-212-4
DOIs
Publication statusPublished - 1995
MoE publication typeA4 Article in a conference publication
EventCellucon '93 - Lund, Sweden
Duration: 21 Jun 199324 Jun 1993

Conference

ConferenceCellucon '93
CountrySweden
CityLund
Period21/06/9324/06/93

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    Teleman, A., Valkeajärvi, A., Koivula, A., Ruohonen, L., & Drakenberg, T. (1995). Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC. In J. F. Kennedy, G. O. Phillips, P. A. Williams, & L. Piculell (Eds.), Cellulose and Cellulose Derivatives: Physicochemical Aspects and Industrial Applications (pp. 37-42). Woodhead Publishing. https://doi.org/10.1533/9781845698539.1.37