Abstract
Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min−1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.8-21 min−1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.
Original language | English |
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Title of host publication | Cellulose and Cellulose Derivatives |
Subtitle of host publication | Physicochemical Aspects and Industrial Applications |
Editors | John F. Kennedy, Glyn O. Phillips, Peter A. Williams, Lennart Piculell |
Place of Publication | Cambridge |
Publisher | Woodhead Publishing |
Pages | 37-42 |
ISBN (Print) | 978-1-85573-212-4 |
DOIs | |
Publication status | Published - 1995 |
MoE publication type | A4 Article in a conference publication |
Event | Cellucon '93 - Lund, Sweden Duration: 21 Jun 1993 → 24 Jun 1993 |
Conference
Conference | Cellucon '93 |
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Country/Territory | Sweden |
City | Lund |
Period | 21/06/93 → 24/06/93 |