Hydrolysis of cellulose and cellooligosaccharides catalysed by cellobiohydrolase II studied by proton NMR and HPLC

Anita Teleman, Anne Valkeajärvi, Anu Koivula, Laura Ruohonen, Torbjörn Drakenberg

    Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

    Abstract

    Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min−1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.8-21 min−1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.
    Original languageEnglish
    Title of host publicationCellulose and Cellulose Derivatives
    Subtitle of host publicationPhysicochemical Aspects and Industrial Applications
    EditorsJohn F. Kennedy, Glyn O. Phillips, Peter A. Williams, Lennart Piculell
    Place of PublicationCambridge
    PublisherWoodhead Publishing
    Pages37-42
    ISBN (Print)978-1-85573-212-4
    DOIs
    Publication statusPublished - 1995
    MoE publication typeA4 Article in a conference publication
    EventCellucon '93 - Lund, Sweden
    Duration: 21 Jun 199324 Jun 1993

    Conference

    ConferenceCellucon '93
    Country/TerritorySweden
    CityLund
    Period21/06/9324/06/93

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