Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC

A. Teleman, A. Valkeajarvi, A. Koivula, L. Ruohonen, T. Drakenberg

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

1 Citation (Scopus)

Abstract

Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min-1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.821 min-1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.

Original languageEnglish
Title of host publicationCellulose and Cellulose Derivatives
Subtitle of host publicationCellucon '93 Proceedings: Physico-Chemical Aspects and Industrial Applications
PublisherWoodhead Publishing
Pages37-42
Number of pages6
ISBN (Print)978-1-85573-212-4
DOIs
Publication statusPublished - 1 Jan 1998
MoE publication typeA4 Article in a conference publication

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Cellulose 1,4-beta-Cellobiosidase
Cellulose
Protons
Hydrolysis
Nuclear magnetic resonance
Substrates
Nuclear magnetic resonance spectroscopy
Glucose
Derivatives

Cite this

Teleman, A., Valkeajarvi, A., Koivula, A., Ruohonen, L., & Drakenberg, T. (1998). Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC. In Cellulose and Cellulose Derivatives: Cellucon '93 Proceedings: Physico-Chemical Aspects and Industrial Applications (pp. 37-42). Woodhead Publishing. https://doi.org/10.1533/9781845698539.1.37
Teleman, A. ; Valkeajarvi, A. ; Koivula, A. ; Ruohonen, L. ; Drakenberg, T. / Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC. Cellulose and Cellulose Derivatives: Cellucon '93 Proceedings: Physico-Chemical Aspects and Industrial Applications. Woodhead Publishing, 1998. pp. 37-42
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Teleman, A, Valkeajarvi, A, Koivula, A, Ruohonen, L & Drakenberg, T 1998, Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC. in Cellulose and Cellulose Derivatives: Cellucon '93 Proceedings: Physico-Chemical Aspects and Industrial Applications. Woodhead Publishing, pp. 37-42. https://doi.org/10.1533/9781845698539.1.37

Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC. / Teleman, A.; Valkeajarvi, A.; Koivula, A.; Ruohonen, L.; Drakenberg, T.

Cellulose and Cellulose Derivatives: Cellucon '93 Proceedings: Physico-Chemical Aspects and Industrial Applications. Woodhead Publishing, 1998. p. 37-42.

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

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T1 - Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC

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AU - Ruohonen, L.

AU - Drakenberg, T.

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N2 - Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min-1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.821 min-1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.

AB - Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min-1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.821 min-1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.

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Teleman A, Valkeajarvi A, Koivula A, Ruohonen L, Drakenberg T. Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC. In Cellulose and Cellulose Derivatives: Cellucon '93 Proceedings: Physico-Chemical Aspects and Industrial Applications. Woodhead Publishing. 1998. p. 37-42 https://doi.org/10.1533/9781845698539.1.37