Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC

A. Teleman; A. Valkeajarvi; Anu Koivula; Laura Ruohonen; Torbjörn Drakenberg

doi:10.1533/9781845698539.1.37

Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC

A. Teleman, A. Valkeajarvi, Anu Koivula, Laura Ruohonen, Torbjörn Drakenberg

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

1 Citation (Scopus)

Abstract

Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min^-1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.821 min^-1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.

Original language	English
Title of host publication	Cellulose and Cellulose Derivatives
Subtitle of host publication	Cellucon '93 Proceedings: Physico-Chemical Aspects and Industrial Applications
Publisher	Woodhead Publishing
Pages	37-42
ISBN (Print)	978-1-85573-212-4
DOIs	https://doi.org/10.1533/9781845698539.1.37
Publication status	Published - 1 Jan 1998
MoE publication type	A4 Article in a conference publication

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Teleman, A., Valkeajarvi, A., Koivula, A., Ruohonen, L., & Drakenberg, T. (1998). Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC. In Cellulose and Cellulose Derivatives: Cellucon '93 Proceedings: Physico-Chemical Aspects and Industrial Applications (pp. 37-42). Woodhead Publishing. https://doi.org/10.1533/9781845698539.1.37

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title = "Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC",

abstract = "Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min-1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.821 min-1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.",

author = "A. Teleman and A. Valkeajarvi and Anu Koivula and Laura Ruohonen and Torbj{\"o}rn Drakenberg",

year = "1998",

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doi = "10.1533/9781845698539.1.37",

language = "English",

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Teleman, A, Valkeajarvi, A, Koivula, A, Ruohonen, L & Drakenberg, T 1998, Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC. in Cellulose and Cellulose Derivatives: Cellucon '93 Proceedings: Physico-Chemical Aspects and Industrial Applications. Woodhead Publishing, pp. 37-42. https://doi.org/10.1533/9781845698539.1.37

Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC. / Teleman, A.; Valkeajarvi, A.; Koivula, Anu et al.
Cellulose and Cellulose Derivatives: Cellucon '93 Proceedings: Physico-Chemical Aspects and Industrial Applications. Woodhead Publishing, 1998. p. 37-42.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

TY - GEN

T1 - Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC

AU - Teleman, A.

AU - Valkeajarvi, A.

AU - Koivula, Anu

AU - Ruohonen, Laura

AU - Drakenberg, Torbjörn

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min-1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.821 min-1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.

AB - Proton NMR spectroscopy and HPLC have been used to investigate the hydrolysis of cellooligosaccharides and cellulose by cellobiohydrolase II from Trichoderma reesei. The cleavage pattern for the substrates cellotriose to cellohexaose has been determined. The catalytic rates were higher for the natural substrates (3.6-300 min-1) than the rates earlier reported for chromophoric cellooligosaccharide derivatives (0.821 min-1). The cellulose hydrolysis is slow compared to the hydrolysis of the soluble cellooligosaccharides. The cellotriose hydrolysis is inhibited by glucose and the inhibition is non-competitive. Cellobiohydrolase II retains maximal activity in a broad pH range, 2.5-6.5.

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DO - 10.1533/9781845698539.1.37

M3 - Conference article in proceedings

AN - SCOPUS:0031619223

SN - 978-1-85573-212-4

SP - 37

EP - 42

BT - Cellulose and Cellulose Derivatives

PB - Woodhead Publishing

ER -

Hydrolysis of cellulose and cellooligosaccharides catalyzed by cellobiohydrolase II studied by proton NMR and HPLC

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