TY - JOUR
T1 - Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase
T2 - Optimization and scale-up
AU - Villaverde, Juan José
AU - van der Vlist, Vincent
AU - Santos, Sónia A.O.
AU - Haarmann, Thomas
AU - Langfelder, Kim
AU - Pirttimaa, Minni
AU - Nyyssölä, Antti
AU - Jylhä, Sirpa
AU - Tamminen, Tarja
AU - Kruus, Kristiina
AU - de Graaff, Leo
AU - Neto, Carlos Pascoal
AU - Simões, Mário M.Q.
AU - Domingues, M.R.M.
AU - Silvestre, Armando J.D.
AU - Eidner, Jasmin
AU - Buchert, Johanna
PY - 2013
Y1 - 2013
N2 - Linoleic acid was converted into hydroperoxides by a Gaeumannomyces graminis tritici lipoxygenase produced recombinantly in Trichoderma reesei. Hydroperoxide production was optimized using a face-centred experimental design in order to study the effects of pH, temperature and time on the conversion of linoleic acid into four regioisomeric hydroperoxyoctadecadienoic acids (HPODE): 13-(Z,E)-, 9-(E,Z)-, 13-(E,E)-, 9-(E,E)-HPODE. Fitting equations described satisfactorily the system behavior and showed that reaction time was the most influencing independent variable. A set of independent variables (pH = 6.7, temperature = 23.9 °C and time = 18 h) allowed to obtain high yields of hydroperoxides (88.0%) with a good selectivity for the 13-(Z,E)-HPODE isomer (47.4%) when the initial substrate concentration was 10 g/L. The production was further investigated using industrially relevant linoleic acid concentrations (100–300 g/L) leading to HPODE yields of ∼40% and the volumetric productivity 3.6 g/(L h), and a selectivity for 13-(Z,E)-HPODE of around 74%.
AB - Linoleic acid was converted into hydroperoxides by a Gaeumannomyces graminis tritici lipoxygenase produced recombinantly in Trichoderma reesei. Hydroperoxide production was optimized using a face-centred experimental design in order to study the effects of pH, temperature and time on the conversion of linoleic acid into four regioisomeric hydroperoxyoctadecadienoic acids (HPODE): 13-(Z,E)-, 9-(E,Z)-, 13-(E,E)-, 9-(E,E)-HPODE. Fitting equations described satisfactorily the system behavior and showed that reaction time was the most influencing independent variable. A set of independent variables (pH = 6.7, temperature = 23.9 °C and time = 18 h) allowed to obtain high yields of hydroperoxides (88.0%) with a good selectivity for the 13-(Z,E)-HPODE isomer (47.4%) when the initial substrate concentration was 10 g/L. The production was further investigated using industrially relevant linoleic acid concentrations (100–300 g/L) leading to HPODE yields of ∼40% and the volumetric productivity 3.6 g/(L h), and a selectivity for 13-(Z,E)-HPODE of around 74%.
KW - Fatty acid hydroperoxides
KW - linoleic acid
KW - lipoxygenase
KW - optimization
KW - scale-up
U2 - 10.1016/j.cej.2012.11.090
DO - 10.1016/j.cej.2012.11.090
M3 - Article
SN - 1385-8947
VL - 217
SP - 82
EP - 90
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
ER -