Hydrophilic modification of polystyrene with hydrophobin for time-resolved immunofluorometric assay

Zefang Wang, Yujian Huang, Shan Li, Haijin Xu (Corresponding Author), Markus Linder (Corresponding Author), Mingqiang Qiao (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

30 Citations (Scopus)

Abstract

Herein we reported that a hydrophobin film was used as a solid support on the polystyrene surface for immobilizing antibodies in the time-resolved immunofluorometric assay (TR-IFMA). Quartz crystal microbalance with dissipative monitoring (QCM-D), X-ray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurements, as well as atomic force microscope (AFM) were used to characterize the hydrophilic modification of polystyrene surface with Class I hydrophobin isolated from Grifola frondosa (HGFI). The performance of HGFI-modified polystyrene was evaluated by TR-IFMA of carcinoembryonic antigen (CEA). QCM-D revealed that HGFI formed an intact monolayer on the polystyrene at pH 5. XPS and WCA measurements showed that self-assembling HGFI could render polystyrene surface hydrophilic for three months. AFM indicated that an end-on antibody monolayer was adsorbed on the HGFI film rather than multilayers on the polystyrene in a side-on orientation. Furthermore, a linear calibration curve (from 5 to 600 ng/mL) of CEA showed HGFI-modified polystyrene had higher detection sensitivity than unmodified ones in TR-IFMA. This present method for modifying polystyrene is simple without severe chemical treatment and may have wide applicability to functionalize other supports for immobilizing biomolecules.
Original languageEnglish
Pages (from-to)1074-1079
JournalBiosensors & Bioelectronics
Volume26
Issue number3
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Fluoroimmunoassay
Polystyrenes
Assays
Quartz Crystal Microbalance Techniques
Photoelectron Spectroscopy
Quartz crystal microbalances
Carcinoembryonic Antigen
Antigens
Angle measurement
Antibodies
Contact angle
Monolayers
Grifola
Microscopes
X ray photoelectron spectroscopy
Water
Monitoring
Biomolecules
Calibration
Multilayers

Keywords

  • Polystyrene
  • hydrophobin
  • immobilization
  • immunoassays
  • wettability

Cite this

Wang, Zefang ; Huang, Yujian ; Li, Shan ; Xu, Haijin ; Linder, Markus ; Qiao, Mingqiang. / Hydrophilic modification of polystyrene with hydrophobin for time-resolved immunofluorometric assay. In: Biosensors & Bioelectronics. 2010 ; Vol. 26, No. 3. pp. 1074-1079.
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Hydrophilic modification of polystyrene with hydrophobin for time-resolved immunofluorometric assay. / Wang, Zefang; Huang, Yujian; Li, Shan; Xu, Haijin (Corresponding Author); Linder, Markus (Corresponding Author); Qiao, Mingqiang (Corresponding Author).

In: Biosensors & Bioelectronics, Vol. 26, No. 3, 2010, p. 1074-1079.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Hydrophilic modification of polystyrene with hydrophobin for time-resolved immunofluorometric assay

AU - Wang, Zefang

AU - Huang, Yujian

AU - Li, Shan

AU - Xu, Haijin

AU - Linder, Markus

AU - Qiao, Mingqiang

PY - 2010

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N2 - Herein we reported that a hydrophobin film was used as a solid support on the polystyrene surface for immobilizing antibodies in the time-resolved immunofluorometric assay (TR-IFMA). Quartz crystal microbalance with dissipative monitoring (QCM-D), X-ray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurements, as well as atomic force microscope (AFM) were used to characterize the hydrophilic modification of polystyrene surface with Class I hydrophobin isolated from Grifola frondosa (HGFI). The performance of HGFI-modified polystyrene was evaluated by TR-IFMA of carcinoembryonic antigen (CEA). QCM-D revealed that HGFI formed an intact monolayer on the polystyrene at pH 5. XPS and WCA measurements showed that self-assembling HGFI could render polystyrene surface hydrophilic for three months. AFM indicated that an end-on antibody monolayer was adsorbed on the HGFI film rather than multilayers on the polystyrene in a side-on orientation. Furthermore, a linear calibration curve (from 5 to 600 ng/mL) of CEA showed HGFI-modified polystyrene had higher detection sensitivity than unmodified ones in TR-IFMA. This present method for modifying polystyrene is simple without severe chemical treatment and may have wide applicability to functionalize other supports for immobilizing biomolecules.

AB - Herein we reported that a hydrophobin film was used as a solid support on the polystyrene surface for immobilizing antibodies in the time-resolved immunofluorometric assay (TR-IFMA). Quartz crystal microbalance with dissipative monitoring (QCM-D), X-ray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurements, as well as atomic force microscope (AFM) were used to characterize the hydrophilic modification of polystyrene surface with Class I hydrophobin isolated from Grifola frondosa (HGFI). The performance of HGFI-modified polystyrene was evaluated by TR-IFMA of carcinoembryonic antigen (CEA). QCM-D revealed that HGFI formed an intact monolayer on the polystyrene at pH 5. XPS and WCA measurements showed that self-assembling HGFI could render polystyrene surface hydrophilic for three months. AFM indicated that an end-on antibody monolayer was adsorbed on the HGFI film rather than multilayers on the polystyrene in a side-on orientation. Furthermore, a linear calibration curve (from 5 to 600 ng/mL) of CEA showed HGFI-modified polystyrene had higher detection sensitivity than unmodified ones in TR-IFMA. This present method for modifying polystyrene is simple without severe chemical treatment and may have wide applicability to functionalize other supports for immobilizing biomolecules.

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