Hydrophobin gene srh1, expressed during sporulation of the biocontrol agent Trichoderma harzianum

Gaston Munoz, Tiina Nakari-Setälä, Eduardo Agosin, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

34 Citations (Scopus)

Abstract

A cDNA clone encoding a spore-related hydrophobin, SRHI, was isolated from a cDNA bank prepared from mRNA induced in sporulating cultures of Trichoderma harzianum by heterologous hybridization using the hfb2 gene encoding a spore-bound hydrophobin of Trichoderma reesei as a probe. Based on sequence similarity the predicted protein was identified as a new member of the class-II hydrophobin family. Including the signal sequences, SRHI has 65% and 56% amino-acid similarity with the T. reesei hydrophobins HFBII and HFBI, respectively, being less similar with other hydrophobins. srh1 is present as one copy in the T. harzianum genome. It is highly expressed under sporulating conditions, both in submerged as well as in aerial cultures. Moreover, nutrient limitation induces srh1 expression.
Original languageEnglish
Pages (from-to)225-230
JournalCurrent Genetics
Volume32
Issue number3
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Spores
Complementary DNA
Protein Sorting Signals
Genes
Clone Cells
Genome
Amino Acids
Food
Messenger RNA
Proteins
1-(heptafluorobutyryl)imidazole

Cite this

@article{f3b556244f6c49e792cd5105d5bebf6e,
title = "Hydrophobin gene srh1, expressed during sporulation of the biocontrol agent Trichoderma harzianum",
abstract = "A cDNA clone encoding a spore-related hydrophobin, SRHI, was isolated from a cDNA bank prepared from mRNA induced in sporulating cultures of Trichoderma harzianum by heterologous hybridization using the hfb2 gene encoding a spore-bound hydrophobin of Trichoderma reesei as a probe. Based on sequence similarity the predicted protein was identified as a new member of the class-II hydrophobin family. Including the signal sequences, SRHI has 65{\%} and 56{\%} amino-acid similarity with the T. reesei hydrophobins HFBII and HFBI, respectively, being less similar with other hydrophobins. srh1 is present as one copy in the T. harzianum genome. It is highly expressed under sporulating conditions, both in submerged as well as in aerial cultures. Moreover, nutrient limitation induces srh1 expression.",
author = "Gaston Munoz and Tiina Nakari-Set{\"a}l{\"a} and Eduardo Agosin and Merja Penttil{\"a}",
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journal = "Current Genetics",
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Hydrophobin gene srh1, expressed during sporulation of the biocontrol agent Trichoderma harzianum. / Munoz, Gaston; Nakari-Setälä, Tiina; Agosin, Eduardo; Penttilä, Merja.

In: Current Genetics, Vol. 32, No. 3, 1997, p. 225-230.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Hydrophobin gene srh1, expressed during sporulation of the biocontrol agent Trichoderma harzianum

AU - Munoz, Gaston

AU - Nakari-Setälä, Tiina

AU - Agosin, Eduardo

AU - Penttilä, Merja

PY - 1997

Y1 - 1997

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AB - A cDNA clone encoding a spore-related hydrophobin, SRHI, was isolated from a cDNA bank prepared from mRNA induced in sporulating cultures of Trichoderma harzianum by heterologous hybridization using the hfb2 gene encoding a spore-bound hydrophobin of Trichoderma reesei as a probe. Based on sequence similarity the predicted protein was identified as a new member of the class-II hydrophobin family. Including the signal sequences, SRHI has 65% and 56% amino-acid similarity with the T. reesei hydrophobins HFBII and HFBI, respectively, being less similar with other hydrophobins. srh1 is present as one copy in the T. harzianum genome. It is highly expressed under sporulating conditions, both in submerged as well as in aerial cultures. Moreover, nutrient limitation induces srh1 expression.

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DO - 10.1007/s002940050270

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