Hydrophobin gene srh1, expressed during sporulation of the biocontrol agent Trichoderma harzianum

Gaston Munoz; Tiina Nakari-Setälä; Eduardo Agosin; Merja Penttilä

doi:10.1007/s002940050270

Hydrophobin gene srh1, expressed during sporulation of the biocontrol agent Trichoderma harzianum

Gaston Munoz, Tiina Nakari-Setälä, Eduardo Agosin, Merja Penttilä

Pontifical Catholic University of Chile

Research output: Contribution to journal › Article › Scientific › peer-review

39 Citations (Scopus)

Abstract

A cDNA clone encoding a spore-related hydrophobin, SRHI, was isolated from a cDNA bank prepared from mRNA induced in sporulating cultures of Trichoderma harzianum by heterologous hybridization using the hfb2 gene encoding a spore-bound hydrophobin of Trichoderma reesei as a probe. Based on sequence similarity the predicted protein was identified as a new member of the class-II hydrophobin family. Including the signal sequences, SRHI has 65% and 56% amino-acid similarity with the T. reesei hydrophobins HFBII and HFBI, respectively, being less similar with other hydrophobins. srh1 is present as one copy in the T. harzianum genome. It is highly expressed under sporulating conditions, both in submerged as well as in aerial cultures. Moreover, nutrient limitation induces srh1 expression.

Original language	English
Pages (from-to)	225-230
Journal	Current Genetics
Volume	32
Issue number	3
DOIs	https://doi.org/10.1007/s002940050270
Publication status	Published - 1997
MoE publication type	A1 Journal article-refereed

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title = "Hydrophobin gene srh1, expressed during sporulation of the biocontrol agent Trichoderma harzianum",

abstract = "A cDNA clone encoding a spore-related hydrophobin, SRHI, was isolated from a cDNA bank prepared from mRNA induced in sporulating cultures of Trichoderma harzianum by heterologous hybridization using the hfb2 gene encoding a spore-bound hydrophobin of Trichoderma reesei as a probe. Based on sequence similarity the predicted protein was identified as a new member of the class-II hydrophobin family. Including the signal sequences, SRHI has 65% and 56% amino-acid similarity with the T. reesei hydrophobins HFBII and HFBI, respectively, being less similar with other hydrophobins. srh1 is present as one copy in the T. harzianum genome. It is highly expressed under sporulating conditions, both in submerged as well as in aerial cultures. Moreover, nutrient limitation induces srh1 expression.",

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T1 - Hydrophobin gene srh1, expressed during sporulation of the biocontrol agent Trichoderma harzianum

AU - Munoz, Gaston

AU - Nakari-Setälä, Tiina

AU - Agosin, Eduardo

AU - Penttilä, Merja

PY - 1997

Y1 - 1997

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AB - A cDNA clone encoding a spore-related hydrophobin, SRHI, was isolated from a cDNA bank prepared from mRNA induced in sporulating cultures of Trichoderma harzianum by heterologous hybridization using the hfb2 gene encoding a spore-bound hydrophobin of Trichoderma reesei as a probe. Based on sequence similarity the predicted protein was identified as a new member of the class-II hydrophobin family. Including the signal sequences, SRHI has 65% and 56% amino-acid similarity with the T. reesei hydrophobins HFBII and HFBI, respectively, being less similar with other hydrophobins. srh1 is present as one copy in the T. harzianum genome. It is highly expressed under sporulating conditions, both in submerged as well as in aerial cultures. Moreover, nutrient limitation induces srh1 expression.

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DO - 10.1007/s002940050270

M3 - Article

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SP - 225

EP - 230

JO - Current Genetics

JF - Current Genetics

IS - 3

ER -

Hydrophobin gene srh1, expressed during sporulation of the biocontrol agent Trichoderma harzianum

Abstract

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