Candida tropicalis Etr1p and Saccharomyces cerevisiae Ybr026p (Mrf1'p), 2-enoyl thioester reductases essential for mitochondrial respiratory competence

Juha Torkko, Kari Koivuranta, Ilkka Miinalainen, Ahmed Yagi, Werner Schmitz, Alexander Kastaniotis, Tomi Airenne, Aner Gurvitz, Kalervo Hiltunen

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Abstract

We report here on the identification and characterization of novel 2-enoyl thioester reductases of fatty acid metabolism, Etr1p from Candida tropicalis and its homolog Ybr026p (Mrf1′p) from Saccharomyces cerevisiae. Overexpression of these proteins in S. cerevisiae led to the development of significantly enlarged mitochondria, whereas deletion of the S. cerevisiae YBR026c gene resulted in rudimentary mitochondria with decreased contents of cytochromes and a respiration-deficient phenotype. Immunolocalization and in vivo targeting experiments showed these proteins to be predominantly mitochondrial. Mitochondrial targeting was essential for complementation of the mutant phenotype, since targeting of the reductases to other subcellular locations failed to reestablish respiratory growth. The mutant phenotype was also complemented by a mitochondrially targeted FabI protein from Escherichia coli. FabI represents a nonhomologous 2-enoyl-acyl carrier protein reductase that participates in the last step of the type II fatty acid synthesis. This indicated that 2-enoyl thioester reductase activity was critical for the mitochondrial function. We conclude that Etr1p and Ybr026p are novel 2-enoyl thioester reductases required for respiration and the maintenance of the mitochondrial compartment, putatively acting in mitochondrial synthesis of fatty acids.
Original languageEnglish
Pages (from-to)6243-6253
Number of pages11
JournalMolecular and Cellular Biology
Volume21
Issue number18
DOIs
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

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Candida tropicalis
Mental Competency
Saccharomyces cerevisiae
Fatty Acids
Phenotype
Mitochondria
Oxidoreductases
Respiration
Cytochromes a
Acyl Carrier Protein
Saccharomyces cerevisiae Proteins
Escherichia coli Proteins
Maintenance
Growth
Genes
trans-2-enoyl-CoA reductase (NADPH)
Proteins

Cite this

Torkko, Juha ; Koivuranta, Kari ; Miinalainen, Ilkka ; Yagi, Ahmed ; Schmitz, Werner ; Kastaniotis, Alexander ; Airenne, Tomi ; Gurvitz, Aner ; Hiltunen, Kalervo. / Candida tropicalis Etr1p and Saccharomyces cerevisiae Ybr026p (Mrf1'p), 2-enoyl thioester reductases essential for mitochondrial respiratory competence. In: Molecular and Cellular Biology. 2001 ; Vol. 21, No. 18. pp. 6243-6253.
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abstract = "We report here on the identification and characterization of novel 2-enoyl thioester reductases of fatty acid metabolism, Etr1p from Candida tropicalis and its homolog Ybr026p (Mrf1′p) from Saccharomyces cerevisiae. Overexpression of these proteins in S. cerevisiae led to the development of significantly enlarged mitochondria, whereas deletion of the S. cerevisiae YBR026c gene resulted in rudimentary mitochondria with decreased contents of cytochromes and a respiration-deficient phenotype. Immunolocalization and in vivo targeting experiments showed these proteins to be predominantly mitochondrial. Mitochondrial targeting was essential for complementation of the mutant phenotype, since targeting of the reductases to other subcellular locations failed to reestablish respiratory growth. The mutant phenotype was also complemented by a mitochondrially targeted FabI protein from Escherichia coli. FabI represents a nonhomologous 2-enoyl-acyl carrier protein reductase that participates in the last step of the type II fatty acid synthesis. This indicated that 2-enoyl thioester reductase activity was critical for the mitochondrial function. We conclude that Etr1p and Ybr026p are novel 2-enoyl thioester reductases required for respiration and the maintenance of the mitochondrial compartment, putatively acting in mitochondrial synthesis of fatty acids.",
author = "Juha Torkko and Kari Koivuranta and Ilkka Miinalainen and Ahmed Yagi and Werner Schmitz and Alexander Kastaniotis and Tomi Airenne and Aner Gurvitz and Kalervo Hiltunen",
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Candida tropicalis Etr1p and Saccharomyces cerevisiae Ybr026p (Mrf1'p), 2-enoyl thioester reductases essential for mitochondrial respiratory competence. / Torkko, Juha; Koivuranta, Kari; Miinalainen, Ilkka; Yagi, Ahmed; Schmitz, Werner; Kastaniotis, Alexander; Airenne, Tomi; Gurvitz, Aner; Hiltunen, Kalervo.

In: Molecular and Cellular Biology, Vol. 21, No. 18, 2001, p. 6243-6253.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Candida tropicalis Etr1p and Saccharomyces cerevisiae Ybr026p (Mrf1'p), 2-enoyl thioester reductases essential for mitochondrial respiratory competence

AU - Torkko, Juha

AU - Koivuranta, Kari

AU - Miinalainen, Ilkka

AU - Yagi, Ahmed

AU - Schmitz, Werner

AU - Kastaniotis, Alexander

AU - Airenne, Tomi

AU - Gurvitz, Aner

AU - Hiltunen, Kalervo

PY - 2001

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N2 - We report here on the identification and characterization of novel 2-enoyl thioester reductases of fatty acid metabolism, Etr1p from Candida tropicalis and its homolog Ybr026p (Mrf1′p) from Saccharomyces cerevisiae. Overexpression of these proteins in S. cerevisiae led to the development of significantly enlarged mitochondria, whereas deletion of the S. cerevisiae YBR026c gene resulted in rudimentary mitochondria with decreased contents of cytochromes and a respiration-deficient phenotype. Immunolocalization and in vivo targeting experiments showed these proteins to be predominantly mitochondrial. Mitochondrial targeting was essential for complementation of the mutant phenotype, since targeting of the reductases to other subcellular locations failed to reestablish respiratory growth. The mutant phenotype was also complemented by a mitochondrially targeted FabI protein from Escherichia coli. FabI represents a nonhomologous 2-enoyl-acyl carrier protein reductase that participates in the last step of the type II fatty acid synthesis. This indicated that 2-enoyl thioester reductase activity was critical for the mitochondrial function. We conclude that Etr1p and Ybr026p are novel 2-enoyl thioester reductases required for respiration and the maintenance of the mitochondrial compartment, putatively acting in mitochondrial synthesis of fatty acids.

AB - We report here on the identification and characterization of novel 2-enoyl thioester reductases of fatty acid metabolism, Etr1p from Candida tropicalis and its homolog Ybr026p (Mrf1′p) from Saccharomyces cerevisiae. Overexpression of these proteins in S. cerevisiae led to the development of significantly enlarged mitochondria, whereas deletion of the S. cerevisiae YBR026c gene resulted in rudimentary mitochondria with decreased contents of cytochromes and a respiration-deficient phenotype. Immunolocalization and in vivo targeting experiments showed these proteins to be predominantly mitochondrial. Mitochondrial targeting was essential for complementation of the mutant phenotype, since targeting of the reductases to other subcellular locations failed to reestablish respiratory growth. The mutant phenotype was also complemented by a mitochondrially targeted FabI protein from Escherichia coli. FabI represents a nonhomologous 2-enoyl-acyl carrier protein reductase that participates in the last step of the type II fatty acid synthesis. This indicated that 2-enoyl thioester reductase activity was critical for the mitochondrial function. We conclude that Etr1p and Ybr026p are novel 2-enoyl thioester reductases required for respiration and the maintenance of the mitochondrial compartment, putatively acting in mitochondrial synthesis of fatty acids.

U2 - 10.1128/MCB.21.18.6243-6253.2001

DO - 10.1128/MCB.21.18.6243-6253.2001

M3 - Article

VL - 21

SP - 6243

EP - 6253

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

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