Identification and characterisation of hydrophobins from Fusarium graminearum

Tuija Sarlin, Teemu Kivioja, Markus Linder, Tiina Nakari-Setälä

Research output: Contribution to conferenceConference articleScientific

Abstract

Fungal infection of barley and malt, particularly by strains of the genus Fusarium, is known to be a direct cause of undesirable beer gushing. Gushing is a phenomenon in which beer spontaneously, without agitation, vigorously over foams out from the package immediately on opening. We have shown previously that small fungal proteins called hydrophobins act as the gushing factors of beer. Hydrophobins have been found on the cell walls of hyphae and on spore surfaces, but they can also be secreted into the culture medium. The characteristic feature of these surface-active proteins is their ability to self-assemble at hydrophilic/hydrophobic interfaces forming amphipathic membranes. Based on sequence comparison, hydrophobins are divided into two different classes, I and II. The aim of our work was to isolate and characterise hydrophobins from a gushing active fungus Fusarium graminearum. We generated profile hidden Markov models (profile HMMs) for the hydrophobin classes IA, IB and II from the multiple sequence alignments of their known members. We searched Fusarium graminearum genome database of predicted proteins (http://www.broad.mit.edu) with the models. The best matching sequences and the corresponding genes were isolated and characterised. One of the putative hydrophobin genes was expressed in Trichoderma reesei and the hydrophobin-like protein was isolated from the culture filtrate of the transformant. A concentration of 0.1 ppm of the RP-HPLC purified protein was observed to induce beer gushing.
Original languageEnglish
Publication statusPublished - 2006
Event9th European Fusarium Seminar - Wageningen, Netherlands
Duration: 19 Sep 200622 Sep 2006

Conference

Conference9th European Fusarium Seminar
CountryNetherlands
CityWageningen
Period19/09/0622/09/06

Fingerprint

Fusarium graminearum
beers
proteins
fungal proteins
Trichoderma reesei
malt
agitation
culture filtrates
sequence alignment
foams
hydrophobins
hyphae
Fusarium
genes
spores
culture media
barley
cell walls
fungi
genome

Cite this

Sarlin, T., Kivioja, T., Linder, M., & Nakari-Setälä, T. (2006). Identification and characterisation of hydrophobins from Fusarium graminearum. Paper presented at 9th European Fusarium Seminar, Wageningen, Netherlands.
Sarlin, Tuija ; Kivioja, Teemu ; Linder, Markus ; Nakari-Setälä, Tiina. / Identification and characterisation of hydrophobins from Fusarium graminearum. Paper presented at 9th European Fusarium Seminar, Wageningen, Netherlands.
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title = "Identification and characterisation of hydrophobins from Fusarium graminearum",
abstract = "Fungal infection of barley and malt, particularly by strains of the genus Fusarium, is known to be a direct cause of undesirable beer gushing. Gushing is a phenomenon in which beer spontaneously, without agitation, vigorously over foams out from the package immediately on opening. We have shown previously that small fungal proteins called hydrophobins act as the gushing factors of beer. Hydrophobins have been found on the cell walls of hyphae and on spore surfaces, but they can also be secreted into the culture medium. The characteristic feature of these surface-active proteins is their ability to self-assemble at hydrophilic/hydrophobic interfaces forming amphipathic membranes. Based on sequence comparison, hydrophobins are divided into two different classes, I and II. The aim of our work was to isolate and characterise hydrophobins from a gushing active fungus Fusarium graminearum. We generated profile hidden Markov models (profile HMMs) for the hydrophobin classes IA, IB and II from the multiple sequence alignments of their known members. We searched Fusarium graminearum genome database of predicted proteins (http://www.broad.mit.edu) with the models. The best matching sequences and the corresponding genes were isolated and characterised. One of the putative hydrophobin genes was expressed in Trichoderma reesei and the hydrophobin-like protein was isolated from the culture filtrate of the transformant. A concentration of 0.1 ppm of the RP-HPLC purified protein was observed to induce beer gushing.",
author = "Tuija Sarlin and Teemu Kivioja and Markus Linder and Tiina Nakari-Set{\"a}l{\"a}",
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note = "9th European Fusarium Seminar ; Conference date: 19-09-2006 Through 22-09-2006",

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Sarlin, T, Kivioja, T, Linder, M & Nakari-Setälä, T 2006, 'Identification and characterisation of hydrophobins from Fusarium graminearum' Paper presented at 9th European Fusarium Seminar, Wageningen, Netherlands, 19/09/06 - 22/09/06, .

Identification and characterisation of hydrophobins from Fusarium graminearum. / Sarlin, Tuija; Kivioja, Teemu; Linder, Markus; Nakari-Setälä, Tiina.

2006. Paper presented at 9th European Fusarium Seminar, Wageningen, Netherlands.

Research output: Contribution to conferenceConference articleScientific

TY - CONF

T1 - Identification and characterisation of hydrophobins from Fusarium graminearum

AU - Sarlin, Tuija

AU - Kivioja, Teemu

AU - Linder, Markus

AU - Nakari-Setälä, Tiina

PY - 2006

Y1 - 2006

N2 - Fungal infection of barley and malt, particularly by strains of the genus Fusarium, is known to be a direct cause of undesirable beer gushing. Gushing is a phenomenon in which beer spontaneously, without agitation, vigorously over foams out from the package immediately on opening. We have shown previously that small fungal proteins called hydrophobins act as the gushing factors of beer. Hydrophobins have been found on the cell walls of hyphae and on spore surfaces, but they can also be secreted into the culture medium. The characteristic feature of these surface-active proteins is their ability to self-assemble at hydrophilic/hydrophobic interfaces forming amphipathic membranes. Based on sequence comparison, hydrophobins are divided into two different classes, I and II. The aim of our work was to isolate and characterise hydrophobins from a gushing active fungus Fusarium graminearum. We generated profile hidden Markov models (profile HMMs) for the hydrophobin classes IA, IB and II from the multiple sequence alignments of their known members. We searched Fusarium graminearum genome database of predicted proteins (http://www.broad.mit.edu) with the models. The best matching sequences and the corresponding genes were isolated and characterised. One of the putative hydrophobin genes was expressed in Trichoderma reesei and the hydrophobin-like protein was isolated from the culture filtrate of the transformant. A concentration of 0.1 ppm of the RP-HPLC purified protein was observed to induce beer gushing.

AB - Fungal infection of barley and malt, particularly by strains of the genus Fusarium, is known to be a direct cause of undesirable beer gushing. Gushing is a phenomenon in which beer spontaneously, without agitation, vigorously over foams out from the package immediately on opening. We have shown previously that small fungal proteins called hydrophobins act as the gushing factors of beer. Hydrophobins have been found on the cell walls of hyphae and on spore surfaces, but they can also be secreted into the culture medium. The characteristic feature of these surface-active proteins is their ability to self-assemble at hydrophilic/hydrophobic interfaces forming amphipathic membranes. Based on sequence comparison, hydrophobins are divided into two different classes, I and II. The aim of our work was to isolate and characterise hydrophobins from a gushing active fungus Fusarium graminearum. We generated profile hidden Markov models (profile HMMs) for the hydrophobin classes IA, IB and II from the multiple sequence alignments of their known members. We searched Fusarium graminearum genome database of predicted proteins (http://www.broad.mit.edu) with the models. The best matching sequences and the corresponding genes were isolated and characterised. One of the putative hydrophobin genes was expressed in Trichoderma reesei and the hydrophobin-like protein was isolated from the culture filtrate of the transformant. A concentration of 0.1 ppm of the RP-HPLC purified protein was observed to induce beer gushing.

M3 - Conference article

ER -

Sarlin T, Kivioja T, Linder M, Nakari-Setälä T. Identification and characterisation of hydrophobins from Fusarium graminearum. 2006. Paper presented at 9th European Fusarium Seminar, Wageningen, Netherlands.