Identification and characterization of a Novel Secondary Metabolite Gene Cluster in Aspergillus nidulans

Kirsi Bromann, Mervi Toivari, Kaarina Viljanen, Anu Vuoristo, Laura Ruohonen, Tiina Nakari-Setälä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Fungal secondary metabolites are a rich source of medically useful compounds due to their pharmaceutical and toxic properties. Sequencing of fungal genomes has revealed numerous secondary metabolite gene clusters, yet products of many of these biosynthetic pathways are unknown since the expression of the clustered genes usually remains silent under normal laboratory conditions. We discovered a novel secondary metabolite in Aspergillus nidulans by predicting a biosynthetic gene cluster with genomic mining. We identified a Zn(II)2Cys6–type transcription factor and demonstrated its role as a pathway‐specific activator for the cluster genes. Quantitative real‐time PCR and DNA array analysis showed that overexpression of the putative transcription factor leads to upregulation of seven genes in the predicted cluster area. The GC/MS analysis of the strain with an activated gene cluster revealed production of a novel secondary metabolite for Aspergillus nidulans. In addition, we noted an increase in sexual fruiting body formation and a reduction in conidiation in the metabolite producing strain. The change in the fungal morphology seen in the overexpression strain could indicate a link between development and activated secondary metabolism.
Original languageEnglish
Title of host publicationAbstracts from the 11th European Conference on Fungal Genetics
PublisherNew Prairie Press
Publication statusPublished - 2012
MoE publication typeNot Eligible
Event11th European Conference on Fungal Genetics - Marburg, Germany
Duration: 30 Mar 20122 Apr 2012

Publication series

NameFungal Genetic Reports
Number6
Volume59

Conference

Conference11th European Conference on Fungal Genetics
CountryGermany
CityMarburg
Period30/03/122/04/12

Fingerprint

Aspergillus nidulans
Multigene Family
Transcription Factors
Fungal Genome
Secondary Metabolism
Poisons
Biosynthetic Pathways
Oligonucleotide Array Sequence Analysis
Up-Regulation
Gene Expression
Polymerase Chain Reaction
Pharmaceutical Preparations
Genes

Cite this

Bromann, K., Toivari, M., Viljanen, K., Vuoristo, A., Ruohonen, L., & Nakari-Setälä, T. (2012). Identification and characterization of a Novel Secondary Metabolite Gene Cluster in Aspergillus nidulans. In Abstracts from the 11th European Conference on Fungal Genetics [PR1.29] New Prairie Press. Fungal Genetic Reports, No. 6, Vol.. 59, Fungal Genetics Reports, No. 6, Vol.. 59
Bromann, Kirsi ; Toivari, Mervi ; Viljanen, Kaarina ; Vuoristo, Anu ; Ruohonen, Laura ; Nakari-Setälä, Tiina. / Identification and characterization of a Novel Secondary Metabolite Gene Cluster in Aspergillus nidulans. Abstracts from the 11th European Conference on Fungal Genetics. New Prairie Press, 2012. (Fungal Genetic Reports; No. 6, Vol. 59). (Fungal Genetics Reports; No. 6, Vol. 59).
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Bromann, K, Toivari, M, Viljanen, K, Vuoristo, A, Ruohonen, L & Nakari-Setälä, T 2012, Identification and characterization of a Novel Secondary Metabolite Gene Cluster in Aspergillus nidulans. in Abstracts from the 11th European Conference on Fungal Genetics., PR1.29, New Prairie Press, Fungal Genetic Reports, no. 6, vol. 59, Fungal Genetics Reports, no. 6, vol. 59, 11th European Conference on Fungal Genetics, Marburg, Germany, 30/03/12.

Identification and characterization of a Novel Secondary Metabolite Gene Cluster in Aspergillus nidulans. / Bromann, Kirsi; Toivari, Mervi; Viljanen, Kaarina; Vuoristo, Anu; Ruohonen, Laura; Nakari-Setälä, Tiina.

Abstracts from the 11th European Conference on Fungal Genetics. New Prairie Press, 2012. PR1.29 (Fungal Genetic Reports; No. 6, Vol. 59). (Fungal Genetics Reports; No. 6, Vol. 59).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Identification and characterization of a Novel Secondary Metabolite Gene Cluster in Aspergillus nidulans

AU - Bromann, Kirsi

AU - Toivari, Mervi

AU - Viljanen, Kaarina

AU - Vuoristo, Anu

AU - Ruohonen, Laura

AU - Nakari-Setälä, Tiina

PY - 2012

Y1 - 2012

N2 - Fungal secondary metabolites are a rich source of medically useful compounds due to their pharmaceutical and toxic properties. Sequencing of fungal genomes has revealed numerous secondary metabolite gene clusters, yet products of many of these biosynthetic pathways are unknown since the expression of the clustered genes usually remains silent under normal laboratory conditions. We discovered a novel secondary metabolite in Aspergillus nidulans by predicting a biosynthetic gene cluster with genomic mining. We identified a Zn(II)2Cys6–type transcription factor and demonstrated its role as a pathway‐specific activator for the cluster genes. Quantitative real‐time PCR and DNA array analysis showed that overexpression of the putative transcription factor leads to upregulation of seven genes in the predicted cluster area. The GC/MS analysis of the strain with an activated gene cluster revealed production of a novel secondary metabolite for Aspergillus nidulans. In addition, we noted an increase in sexual fruiting body formation and a reduction in conidiation in the metabolite producing strain. The change in the fungal morphology seen in the overexpression strain could indicate a link between development and activated secondary metabolism.

AB - Fungal secondary metabolites are a rich source of medically useful compounds due to their pharmaceutical and toxic properties. Sequencing of fungal genomes has revealed numerous secondary metabolite gene clusters, yet products of many of these biosynthetic pathways are unknown since the expression of the clustered genes usually remains silent under normal laboratory conditions. We discovered a novel secondary metabolite in Aspergillus nidulans by predicting a biosynthetic gene cluster with genomic mining. We identified a Zn(II)2Cys6–type transcription factor and demonstrated its role as a pathway‐specific activator for the cluster genes. Quantitative real‐time PCR and DNA array analysis showed that overexpression of the putative transcription factor leads to upregulation of seven genes in the predicted cluster area. The GC/MS analysis of the strain with an activated gene cluster revealed production of a novel secondary metabolite for Aspergillus nidulans. In addition, we noted an increase in sexual fruiting body formation and a reduction in conidiation in the metabolite producing strain. The change in the fungal morphology seen in the overexpression strain could indicate a link between development and activated secondary metabolism.

M3 - Conference abstract in proceedings

T3 - Fungal Genetic Reports

BT - Abstracts from the 11th European Conference on Fungal Genetics

PB - New Prairie Press

ER -

Bromann K, Toivari M, Viljanen K, Vuoristo A, Ruohonen L, Nakari-Setälä T. Identification and characterization of a Novel Secondary Metabolite Gene Cluster in Aspergillus nidulans. In Abstracts from the 11th European Conference on Fungal Genetics. New Prairie Press. 2012. PR1.29. (Fungal Genetic Reports; No. 6, Vol. 59). (Fungal Genetics Reports; No. 6, Vol. 59).