Identification and characterization of hydrophobins from Fusarium graminearum

Tuija Sarlin, Teemu Kivioja, Markus Linder, Tiina Nakari-Setälä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Fungal infection of barley and malt, particularly by strains of the genus Fusarium, is known to be a direct cause of undesirable beer gushing. Gushing is a phenomenon in which beer spontaneously, without agitation, vigorously over foams out from the package immediately on opening. We have shown previously that small fungal proteins, hydrophobins, act as gushing factors in beer. The aim of our work was to isolate and characterise hydrophobins from a gushing active fungus Fusarium graminearum. We generated profile hidden Markov models (profile HMMs) for the hydrophobin classes IA, IB and II from the multiple sequence alignments of their known members. We searched Fusarium graminearum genome database of predicted proteins ( http://www.broad.mit.edu ) with the models. The best matching sequences and the corresponding genes were isolated and characterised. One of the putative hydrophobin genes was expressed in Trichoderma reesei and the hydrophobinlike protein was isolated from the culture filtrate of the transformant. A concentration of 0.03 ppm of the RP-HPLC purified protein was observed to induce beer gushing.
Original languageEnglish
Title of host publication8th European Conference on Fungal Genetics
Subtitle of host publicationBook of Abstracts
PublisherTechnische Universität Wien
Publication statusPublished - 2006
Event8th European Conference on Fungal Genetics - Vienna, Austria
Duration: 8 Apr 200611 Apr 2006

Conference

Conference8th European Conference on Fungal Genetics
CountryAustria
CityVienna
Period8/04/0611/04/06

Fingerprint

Fusarium graminearum
beers
fungal proteins
Trichoderma reesei
proteins
malt
agitation
culture filtrates
sequence alignment
foams
Fusarium
genes
barley
fungi
genome
hydrophobins
infection

Cite this

Sarlin, T., Kivioja, T., Linder, M., & Nakari-Setälä, T. (2006). Identification and characterization of hydrophobins from Fusarium graminearum. In 8th European Conference on Fungal Genetics: Book of Abstracts [VIIp-8 ] Technische Universität Wien.
Sarlin, Tuija ; Kivioja, Teemu ; Linder, Markus ; Nakari-Setälä, Tiina. / Identification and characterization of hydrophobins from Fusarium graminearum. 8th European Conference on Fungal Genetics: Book of Abstracts. Technische Universität Wien, 2006.
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Sarlin, T, Kivioja, T, Linder, M & Nakari-Setälä, T 2006, Identification and characterization of hydrophobins from Fusarium graminearum. in 8th European Conference on Fungal Genetics: Book of Abstracts., VIIp-8 , Technische Universität Wien, 8th European Conference on Fungal Genetics, Vienna, Austria, 8/04/06.

Identification and characterization of hydrophobins from Fusarium graminearum. / Sarlin, Tuija; Kivioja, Teemu; Linder, Markus; Nakari-Setälä, Tiina.

8th European Conference on Fungal Genetics: Book of Abstracts. Technische Universität Wien, 2006. VIIp-8 .

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Identification and characterization of hydrophobins from Fusarium graminearum

AU - Sarlin, Tuija

AU - Kivioja, Teemu

AU - Linder, Markus

AU - Nakari-Setälä, Tiina

N1 - Poster

PY - 2006

Y1 - 2006

N2 - Fungal infection of barley and malt, particularly by strains of the genus Fusarium, is known to be a direct cause of undesirable beer gushing. Gushing is a phenomenon in which beer spontaneously, without agitation, vigorously over foams out from the package immediately on opening. We have shown previously that small fungal proteins, hydrophobins, act as gushing factors in beer. The aim of our work was to isolate and characterise hydrophobins from a gushing active fungus Fusarium graminearum. We generated profile hidden Markov models (profile HMMs) for the hydrophobin classes IA, IB and II from the multiple sequence alignments of their known members. We searched Fusarium graminearum genome database of predicted proteins ( http://www.broad.mit.edu ) with the models. The best matching sequences and the corresponding genes were isolated and characterised. One of the putative hydrophobin genes was expressed in Trichoderma reesei and the hydrophobinlike protein was isolated from the culture filtrate of the transformant. A concentration of 0.03 ppm of the RP-HPLC purified protein was observed to induce beer gushing.

AB - Fungal infection of barley and malt, particularly by strains of the genus Fusarium, is known to be a direct cause of undesirable beer gushing. Gushing is a phenomenon in which beer spontaneously, without agitation, vigorously over foams out from the package immediately on opening. We have shown previously that small fungal proteins, hydrophobins, act as gushing factors in beer. The aim of our work was to isolate and characterise hydrophobins from a gushing active fungus Fusarium graminearum. We generated profile hidden Markov models (profile HMMs) for the hydrophobin classes IA, IB and II from the multiple sequence alignments of their known members. We searched Fusarium graminearum genome database of predicted proteins ( http://www.broad.mit.edu ) with the models. The best matching sequences and the corresponding genes were isolated and characterised. One of the putative hydrophobin genes was expressed in Trichoderma reesei and the hydrophobinlike protein was isolated from the culture filtrate of the transformant. A concentration of 0.03 ppm of the RP-HPLC purified protein was observed to induce beer gushing.

M3 - Conference abstract in proceedings

BT - 8th European Conference on Fungal Genetics

PB - Technische Universität Wien

ER -

Sarlin T, Kivioja T, Linder M, Nakari-Setälä T. Identification and characterization of hydrophobins from Fusarium graminearum. In 8th European Conference on Fungal Genetics: Book of Abstracts. Technische Universität Wien. 2006. VIIp-8