Identification in the mould Hypocrea jecorina of a gene encoding an NADP+: D-xylose dehydrogenase

Suvi Berghäll, Satu Hilditch, Merja Penttilä, Peter Richard

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Abstract

A gene coding for an NADP+-dependent d-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with d-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP+. The Km values for d-xylose and NADP+ are 43 mM and 250 μM, respectively. The role of this enzyme in H. jecorina is unclear because in this organism d-xylose is predominantly catabolized through a path with xylitol and d-xylulose as intermediates and the mould is unable to grow on d-xylonic acid.

Original languageEnglish
Pages (from-to)249-253
JournalFEMS Microbiology Letters
Volume277
Issue number2
DOIs
Publication statusPublished - 1 Dec 2007
MoE publication typeA1 Journal article-refereed

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Keywords

  • D-xylose dehydrogenase
  • D-xylose metabolism
  • EC 1.1.1.175
  • Hypocrea jecorina
  • NADP-linked D-xylose dehydrogenase
  • Trichoderma reesei

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