Identification in the mould Hypocrea jecorina of a gene encoding an NADP+: D-xylose dehydrogenase

Suvi Berghäll; Satu Hilditch; Merja Penttilä; Peter Richard

doi:10.1111/j.1574-6968.2007.00969.x

Identification in the mould Hypocrea jecorina of a gene encoding an NADP⁺: D-xylose dehydrogenase

Suvi Berghäll, Satu Hilditch, Merja Penttilä, Peter Richard

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

A gene coding for an NADP⁺-dependent d-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with d-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP⁺. The K_m values for d-xylose and NADP⁺ are 43 mM and 250 μM, respectively. The role of this enzyme in H. jecorina is unclear because in this organism d-xylose is predominantly catabolized through a path with xylitol and d-xylulose as intermediates and the mould is unable to grow on d-xylonic acid.

Original language	English
Pages (from-to)	249-253
Journal	FEMS Microbiology Letters
Volume	277
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2007.00969.x
Publication status	Published - 1 Dec 2007
MoE publication type	A1 Journal article-refereed

Keywords

D-xylose dehydrogenase
D-xylose metabolism
EC 1.1.1.175
Hypocrea jecorina
NADP-linked D-xylose dehydrogenase
Trichoderma reesei

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title = "Identification in the mould Hypocrea jecorina of a gene encoding an NADP+: D-xylose dehydrogenase",

abstract = "A gene coding for an NADP+-dependent d-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with d-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP+. The Km values for d-xylose and NADP+ are 43 mM and 250 μM, respectively. The role of this enzyme in H. jecorina is unclear because in this organism d-xylose is predominantly catabolized through a path with xylitol and d-xylulose as intermediates and the mould is unable to grow on d-xylonic acid.",

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AU - Hilditch, Satu

AU - Penttilä, Merja

AU - Richard, Peter

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AB - A gene coding for an NADP+-dependent d-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with d-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP+. The Km values for d-xylose and NADP+ are 43 mM and 250 μM, respectively. The role of this enzyme in H. jecorina is unclear because in this organism d-xylose is predominantly catabolized through a path with xylitol and d-xylulose as intermediates and the mould is unable to grow on d-xylonic acid.

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IS - 2

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Identification in the mould Hypocrea jecorina of a gene encoding an NADP⁺: D-xylose dehydrogenase

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