Abstract
A gene coding for an NADP+-dependent d-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with d-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP+. The Km values for d-xylose and NADP+ are 43 mM and 250 μM, respectively. The role of this enzyme in H. jecorina is unclear because in this organism d-xylose is predominantly catabolized through a path with xylitol and d-xylulose as intermediates and the mould is unable to grow on d-xylonic acid.
Original language | English |
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Pages (from-to) | 249-253 |
Journal | FEMS Microbiology Letters |
Volume | 277 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Dec 2007 |
MoE publication type | A1 Journal article-refereed |
Keywords
- D-xylose dehydrogenase
- D-xylose metabolism
- EC 1.1.1.175
- Hypocrea jecorina
- NADP-linked D-xylose dehydrogenase
- Trichoderma reesei