Abstract
The first enzyme in the pathway for L-arabinose catabolism in eukaryotic microorganisms is a reductase, reducing L-arabinose to L-arabitol. The enzymes catalyzing this reduction are in general nonspecific and would also reduce D-xylose to xylitol, the first step in eukaryotic D-xylose catabolism. It is not clear whether microorganisms use different enzymes depending on the carbon source. Here we show that Aspergillus niger makes use of two different enzymes. We identified, cloned, and characterized an L-arabinose reductase, larA, that is different from the D-xylose reductase, xyrA. The larA is up-regulated on L-arabinose, while the xyrA is up-regulated on D-xylose. There is however an initial up-regulation of larA also on D-xylose but that fades away after about 4 h. The deletion of the larA gene in A. niger results in a slow growth phenotype on L-arabinose, whereas the growth on D-xylose is unaffected. The L-arabinose reductase can convert L-arabinose and D-xylose to their corresponding sugar alcohols but has a higher affinity for L-arabinose. The Km for L-arabinose is 54 ± 6 mM and for D-xylose 155 ± 15 mM.
| Original language | English |
|---|---|
| Pages (from-to) | 23622-23628 |
| Number of pages | 7 |
| Journal | Journal of Biological Chemistry |
| Volume | 285 |
| Issue number | 31 |
| DOIs | |
| Publication status | Published - 30 Jul 2010 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Aspergillus niger
- pentose catabolism
- regulation of pentose catabolism
- aldose reductase
- EC.1.1.1.21
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Identification of an L-arabinose reductase gene in Aspergillus niger and its role in L-arabinose catabolism. / Mojzita, Dominik; Penttilä, Merja; Richard, Peter.
In: Journal of Biological Chemistry, Vol. 285, No. 31, 30.07.2010, p. 23622-23628.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Identification of an L-arabinose reductase gene in Aspergillus niger and its role in L-arabinose catabolism
AU - Mojzita, Dominik
AU - Penttilä, Merja
AU - Richard, Peter
N1 - CA2: TK402 CA2: TK400 ISI: BIOCHEMISTRY & MOLECULAR BIOLOGY
PY - 2010/7/30
Y1 - 2010/7/30
N2 - The first enzyme in the pathway for L-arabinose catabolism in eukaryotic microorganisms is a reductase, reducing L-arabinose to L-arabitol. The enzymes catalyzing this reduction are in general nonspecific and would also reduce D-xylose to xylitol, the first step in eukaryotic D-xylose catabolism. It is not clear whether microorganisms use different enzymes depending on the carbon source. Here we show that Aspergillus niger makes use of two different enzymes. We identified, cloned, and characterized an L-arabinose reductase, larA, that is different from the D-xylose reductase, xyrA. The larA is up-regulated on L-arabinose, while the xyrA is up-regulated on D-xylose. There is however an initial up-regulation of larA also on D-xylose but that fades away after about 4 h. The deletion of the larA gene in A. niger results in a slow growth phenotype on L-arabinose, whereas the growth on D-xylose is unaffected. The L-arabinose reductase can convert L-arabinose and D-xylose to their corresponding sugar alcohols but has a higher affinity for L-arabinose. The Km for L-arabinose is 54 ± 6 mM and for D-xylose 155 ± 15 mM.
AB - The first enzyme in the pathway for L-arabinose catabolism in eukaryotic microorganisms is a reductase, reducing L-arabinose to L-arabitol. The enzymes catalyzing this reduction are in general nonspecific and would also reduce D-xylose to xylitol, the first step in eukaryotic D-xylose catabolism. It is not clear whether microorganisms use different enzymes depending on the carbon source. Here we show that Aspergillus niger makes use of two different enzymes. We identified, cloned, and characterized an L-arabinose reductase, larA, that is different from the D-xylose reductase, xyrA. The larA is up-regulated on L-arabinose, while the xyrA is up-regulated on D-xylose. There is however an initial up-regulation of larA also on D-xylose but that fades away after about 4 h. The deletion of the larA gene in A. niger results in a slow growth phenotype on L-arabinose, whereas the growth on D-xylose is unaffected. The L-arabinose reductase can convert L-arabinose and D-xylose to their corresponding sugar alcohols but has a higher affinity for L-arabinose. The Km for L-arabinose is 54 ± 6 mM and for D-xylose 155 ± 15 mM.
KW - Aspergillus niger
KW - pentose catabolism
KW - regulation of pentose catabolism
KW - aldose reductase
KW - EC.1.1.1.21
UR - http://www.scopus.com/inward/record.url?scp=77954911034&partnerID=8YFLogxK
U2 - 10.1074/jbc.M110.113399
DO - 10.1074/jbc.M110.113399
M3 - Article
C2 - 20511228
AN - SCOPUS:77954911034
VL - 285
SP - 23622
EP - 23628
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 31
ER -