Identification of indole alkaloids of Catharanthus roseus with LC/MS using collision-induced dissociation with the thermospray ion repeller

Seppo Auriola, Risto Kostiainen, Toivo Naaranlahti, Seppo Lapinjoki

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Fragmentation of protonated molecular ions produced from catharanthine, tabersonine, ajmalicine and serpentine in a high‐performance liquid chromatography (HPLC) thermospray ion source was studied. Collision‐induced dissociation (CID) of the compounds was achieved by merely increasing the repeller potential; i.e. no discharge current was applied and filament was not on. Proportion of fragments to the protonated molecular ions increased with the potential at the 180–350 V range studied, but overall yield of detected ions decreased at the higher voltages in all cases. Comparison of fragments produced in the thermospray CID with those derived from the protonated molecular ions by colliding with argon in a triple‐stage quadrupole instrument showed that the fragmentation is very similar in both cases. An analytical application for identifying the alkaloids from plant cell culture material by HPLC/mass spectrometry using the thermospray CID is described.
Original languageEnglish
Pages (from-to)400-404
JournalBiomedical and Environmental Mass Spectrometry
Volume19
DOIs
Publication statusPublished - 1990
MoE publication typeA1 Journal article-refereed

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Indole Alkaloids
Ions
Liquid chromatography
Plant cell culture
Argon
Ion sources
Mass spectrometry
Electric potential

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title = "Identification of indole alkaloids of Catharanthus roseus with LC/MS using collision-induced dissociation with the thermospray ion repeller",
abstract = "Fragmentation of protonated molecular ions produced from catharanthine, tabersonine, ajmalicine and serpentine in a high‐performance liquid chromatography (HPLC) thermospray ion source was studied. Collision‐induced dissociation (CID) of the compounds was achieved by merely increasing the repeller potential; i.e. no discharge current was applied and filament was not on. Proportion of fragments to the protonated molecular ions increased with the potential at the 180–350 V range studied, but overall yield of detected ions decreased at the higher voltages in all cases. Comparison of fragments produced in the thermospray CID with those derived from the protonated molecular ions by colliding with argon in a triple‐stage quadrupole instrument showed that the fragmentation is very similar in both cases. An analytical application for identifying the alkaloids from plant cell culture material by HPLC/mass spectrometry using the thermospray CID is described.",
author = "Seppo Auriola and Risto Kostiainen and Toivo Naaranlahti and Seppo Lapinjoki",
year = "1990",
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language = "English",
volume = "19",
pages = "400--404",
journal = "Journal of Mass Spectrometry",
issn = "1076-5174",
publisher = "Wiley-Blackwell",

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Identification of indole alkaloids of Catharanthus roseus with LC/MS using collision-induced dissociation with the thermospray ion repeller. / Auriola, Seppo; Kostiainen, Risto; Naaranlahti, Toivo; Lapinjoki, Seppo.

In: Biomedical and Environmental Mass Spectrometry, Vol. 19, 1990, p. 400-404.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Identification of indole alkaloids of Catharanthus roseus with LC/MS using collision-induced dissociation with the thermospray ion repeller

AU - Auriola, Seppo

AU - Kostiainen, Risto

AU - Naaranlahti, Toivo

AU - Lapinjoki, Seppo

PY - 1990

Y1 - 1990

N2 - Fragmentation of protonated molecular ions produced from catharanthine, tabersonine, ajmalicine and serpentine in a high‐performance liquid chromatography (HPLC) thermospray ion source was studied. Collision‐induced dissociation (CID) of the compounds was achieved by merely increasing the repeller potential; i.e. no discharge current was applied and filament was not on. Proportion of fragments to the protonated molecular ions increased with the potential at the 180–350 V range studied, but overall yield of detected ions decreased at the higher voltages in all cases. Comparison of fragments produced in the thermospray CID with those derived from the protonated molecular ions by colliding with argon in a triple‐stage quadrupole instrument showed that the fragmentation is very similar in both cases. An analytical application for identifying the alkaloids from plant cell culture material by HPLC/mass spectrometry using the thermospray CID is described.

AB - Fragmentation of protonated molecular ions produced from catharanthine, tabersonine, ajmalicine and serpentine in a high‐performance liquid chromatography (HPLC) thermospray ion source was studied. Collision‐induced dissociation (CID) of the compounds was achieved by merely increasing the repeller potential; i.e. no discharge current was applied and filament was not on. Proportion of fragments to the protonated molecular ions increased with the potential at the 180–350 V range studied, but overall yield of detected ions decreased at the higher voltages in all cases. Comparison of fragments produced in the thermospray CID with those derived from the protonated molecular ions by colliding with argon in a triple‐stage quadrupole instrument showed that the fragmentation is very similar in both cases. An analytical application for identifying the alkaloids from plant cell culture material by HPLC/mass spectrometry using the thermospray CID is described.

U2 - 10.1002/bms.1200190704

DO - 10.1002/bms.1200190704

M3 - Article

VL - 19

SP - 400

EP - 404

JO - Journal of Mass Spectrometry

JF - Journal of Mass Spectrometry

SN - 1076-5174

ER -