Identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected Lactobacillus strains

Estefania F. Garcia, Winnie A. Luciano, Danilo E. Xavier, Whyara C.A. da Costa, Kleber de Sousa Oliveira, Octavio L. Franco, Marcos A. de Morais Junior, Brigida T.L. Lucena, Renata C. Picao, Marciane Magnani, Maria Saarela, Evandro L. de Souza (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

28 Citations (Scopus)

Abstract

This study aimed to identify lactic acid bacteria (LAB) in byproducts of fruit (Malpighia glabra L., Mangifera indica L., Annona muricata L., and Fragaria vesca L.) pulp processing. Fifty strains of LAB were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequence (16S rRNA) analysis. Species belonging to Lactobacillus genus were the predominant LAB in all fruit pulp processing byproducts. The average congruency between the MALDI-TOF MS and 16S rRNA in LAB species identification reached 86%. Isolates of L. plantarum, L. brevis, L. pentosus, L. lactis and L. mesenteroides were identified with 100% congruency. MALDI-TOF MS and 16S rRNA analysis presented 86 and 100% efficiency of LAB species identification, respectively. Further, five selected Lactobacillus strains (L. brevis 59, L. pentosus 129, L. paracasei 108, L. plantarum 49, and L. fermentum 111) were evaluated for desirable probiotic-related properties and growth behavior on two different cultivation media. The exposure to pH 2.0 sharply decreased the counts of the different Lactobacillus strains after a 1 or 2 h incubation, while varied decreases were noted after 3 h of exposure to pH 3.0. Overall, the exposure to pH 5.0 and to bile salts (0.15, 0.30, and 1.00%) did not decrease the counts of the Lactobacillus strains. All tested Lactobacillus strains presented inhibitory activity against Staphylococcus aureus, Salmonella Typhimurium, Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli, and presented variable susceptibility to different antibiotics. The selected Lactobacillus strains presented satisfactory and reproducible growth behavior. In conclusion, MALDI-TOF MS and 16S rRNA analysis revealed high efficiency and congruency for LAB species identification, and the selected Lactobacillus strains may be candidates for further investigation of novel probiotic strains.
Original languageEnglish
Article number1371
JournalFrontiers in Microbiology
Volume7
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

Probiotics
Lactobacillus
Lactic Acid
Fruit
Bacteria
Mass Spectrometry
Lasers
Malpighiaceae
Annona
Mangifera
Fragaria
Salmonella enteritidis
Listeria monocytogenes
Salmonella typhimurium
Growth
Bile Acids and Salts
rRNA Genes
Staphylococcus aureus
Escherichia coli
Anti-Bacterial Agents

Keywords

  • 16S rRNA gene sequencing
  • Fruit
  • Lactobacillus
  • MALDI-TOF profiling
  • Probiotic

Cite this

Garcia, E. F., Luciano, W. A., Xavier, D. E., da Costa, W. C. A., de Sousa Oliveira, K., Franco, O. L., ... de Souza, E. L. (2016). Identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected Lactobacillus strains. Frontiers in Microbiology, 7, [1371]. https://doi.org/10.3389/fmicb.2016.01371
Garcia, Estefania F. ; Luciano, Winnie A. ; Xavier, Danilo E. ; da Costa, Whyara C.A. ; de Sousa Oliveira, Kleber ; Franco, Octavio L. ; de Morais Junior, Marcos A. ; Lucena, Brigida T.L. ; Picao, Renata C. ; Magnani, Marciane ; Saarela, Maria ; de Souza, Evandro L. / Identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected Lactobacillus strains. In: Frontiers in Microbiology. 2016 ; Vol. 7.
@article{e8a675dd6e1b4807a5ad297abd1ca94d,
title = "Identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected Lactobacillus strains",
abstract = "This study aimed to identify lactic acid bacteria (LAB) in byproducts of fruit (Malpighia glabra L., Mangifera indica L., Annona muricata L., and Fragaria vesca L.) pulp processing. Fifty strains of LAB were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequence (16S rRNA) analysis. Species belonging to Lactobacillus genus were the predominant LAB in all fruit pulp processing byproducts. The average congruency between the MALDI-TOF MS and 16S rRNA in LAB species identification reached 86{\%}. Isolates of L. plantarum, L. brevis, L. pentosus, L. lactis and L. mesenteroides were identified with 100{\%} congruency. MALDI-TOF MS and 16S rRNA analysis presented 86 and 100{\%} efficiency of LAB species identification, respectively. Further, five selected Lactobacillus strains (L. brevis 59, L. pentosus 129, L. paracasei 108, L. plantarum 49, and L. fermentum 111) were evaluated for desirable probiotic-related properties and growth behavior on two different cultivation media. The exposure to pH 2.0 sharply decreased the counts of the different Lactobacillus strains after a 1 or 2 h incubation, while varied decreases were noted after 3 h of exposure to pH 3.0. Overall, the exposure to pH 5.0 and to bile salts (0.15, 0.30, and 1.00{\%}) did not decrease the counts of the Lactobacillus strains. All tested Lactobacillus strains presented inhibitory activity against Staphylococcus aureus, Salmonella Typhimurium, Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli, and presented variable susceptibility to different antibiotics. The selected Lactobacillus strains presented satisfactory and reproducible growth behavior. In conclusion, MALDI-TOF MS and 16S rRNA analysis revealed high efficiency and congruency for LAB species identification, and the selected Lactobacillus strains may be candidates for further investigation of novel probiotic strains.",
keywords = "16S rRNA gene sequencing, Fruit, Lactobacillus, MALDI-TOF profiling, Probiotic",
author = "Garcia, {Estefania F.} and Luciano, {Winnie A.} and Xavier, {Danilo E.} and {da Costa}, {Whyara C.A.} and {de Sousa Oliveira}, Kleber and Franco, {Octavio L.} and {de Morais Junior}, {Marcos A.} and Lucena, {Brigida T.L.} and Picao, {Renata C.} and Marciane Magnani and Maria Saarela and {de Souza}, {Evandro L.}",
year = "2016",
doi = "10.3389/fmicb.2016.01371",
language = "English",
volume = "7",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media",

}

Garcia, EF, Luciano, WA, Xavier, DE, da Costa, WCA, de Sousa Oliveira, K, Franco, OL, de Morais Junior, MA, Lucena, BTL, Picao, RC, Magnani, M, Saarela, M & de Souza, EL 2016, 'Identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected Lactobacillus strains', Frontiers in Microbiology, vol. 7, 1371. https://doi.org/10.3389/fmicb.2016.01371

Identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected Lactobacillus strains. / Garcia, Estefania F.; Luciano, Winnie A.; Xavier, Danilo E.; da Costa, Whyara C.A.; de Sousa Oliveira, Kleber; Franco, Octavio L.; de Morais Junior, Marcos A.; Lucena, Brigida T.L.; Picao, Renata C.; Magnani, Marciane; Saarela, Maria; de Souza, Evandro L. (Corresponding Author).

In: Frontiers in Microbiology, Vol. 7, 1371, 2016.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected Lactobacillus strains

AU - Garcia, Estefania F.

AU - Luciano, Winnie A.

AU - Xavier, Danilo E.

AU - da Costa, Whyara C.A.

AU - de Sousa Oliveira, Kleber

AU - Franco, Octavio L.

AU - de Morais Junior, Marcos A.

AU - Lucena, Brigida T.L.

AU - Picao, Renata C.

AU - Magnani, Marciane

AU - Saarela, Maria

AU - de Souza, Evandro L.

PY - 2016

Y1 - 2016

N2 - This study aimed to identify lactic acid bacteria (LAB) in byproducts of fruit (Malpighia glabra L., Mangifera indica L., Annona muricata L., and Fragaria vesca L.) pulp processing. Fifty strains of LAB were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequence (16S rRNA) analysis. Species belonging to Lactobacillus genus were the predominant LAB in all fruit pulp processing byproducts. The average congruency between the MALDI-TOF MS and 16S rRNA in LAB species identification reached 86%. Isolates of L. plantarum, L. brevis, L. pentosus, L. lactis and L. mesenteroides were identified with 100% congruency. MALDI-TOF MS and 16S rRNA analysis presented 86 and 100% efficiency of LAB species identification, respectively. Further, five selected Lactobacillus strains (L. brevis 59, L. pentosus 129, L. paracasei 108, L. plantarum 49, and L. fermentum 111) were evaluated for desirable probiotic-related properties and growth behavior on two different cultivation media. The exposure to pH 2.0 sharply decreased the counts of the different Lactobacillus strains after a 1 or 2 h incubation, while varied decreases were noted after 3 h of exposure to pH 3.0. Overall, the exposure to pH 5.0 and to bile salts (0.15, 0.30, and 1.00%) did not decrease the counts of the Lactobacillus strains. All tested Lactobacillus strains presented inhibitory activity against Staphylococcus aureus, Salmonella Typhimurium, Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli, and presented variable susceptibility to different antibiotics. The selected Lactobacillus strains presented satisfactory and reproducible growth behavior. In conclusion, MALDI-TOF MS and 16S rRNA analysis revealed high efficiency and congruency for LAB species identification, and the selected Lactobacillus strains may be candidates for further investigation of novel probiotic strains.

AB - This study aimed to identify lactic acid bacteria (LAB) in byproducts of fruit (Malpighia glabra L., Mangifera indica L., Annona muricata L., and Fragaria vesca L.) pulp processing. Fifty strains of LAB were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequence (16S rRNA) analysis. Species belonging to Lactobacillus genus were the predominant LAB in all fruit pulp processing byproducts. The average congruency between the MALDI-TOF MS and 16S rRNA in LAB species identification reached 86%. Isolates of L. plantarum, L. brevis, L. pentosus, L. lactis and L. mesenteroides were identified with 100% congruency. MALDI-TOF MS and 16S rRNA analysis presented 86 and 100% efficiency of LAB species identification, respectively. Further, five selected Lactobacillus strains (L. brevis 59, L. pentosus 129, L. paracasei 108, L. plantarum 49, and L. fermentum 111) were evaluated for desirable probiotic-related properties and growth behavior on two different cultivation media. The exposure to pH 2.0 sharply decreased the counts of the different Lactobacillus strains after a 1 or 2 h incubation, while varied decreases were noted after 3 h of exposure to pH 3.0. Overall, the exposure to pH 5.0 and to bile salts (0.15, 0.30, and 1.00%) did not decrease the counts of the Lactobacillus strains. All tested Lactobacillus strains presented inhibitory activity against Staphylococcus aureus, Salmonella Typhimurium, Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli, and presented variable susceptibility to different antibiotics. The selected Lactobacillus strains presented satisfactory and reproducible growth behavior. In conclusion, MALDI-TOF MS and 16S rRNA analysis revealed high efficiency and congruency for LAB species identification, and the selected Lactobacillus strains may be candidates for further investigation of novel probiotic strains.

KW - 16S rRNA gene sequencing

KW - Fruit

KW - Lactobacillus

KW - MALDI-TOF profiling

KW - Probiotic

U2 - 10.3389/fmicb.2016.01371

DO - 10.3389/fmicb.2016.01371

M3 - Article

VL - 7

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 1371

ER -