TY - JOUR
T1 - Identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected Lactobacillus strains
AU - Garcia, Estefania F.
AU - Luciano, Winnie A.
AU - Xavier, Danilo E.
AU - da Costa, Whyara C.A.
AU - de Sousa Oliveira, Kleber
AU - Franco, Octavio L.
AU - de Morais Junior, Marcos A.
AU - Lucena, Brigida T.L.
AU - Picao, Renata C.
AU - Magnani, Marciane
AU - Saarela, Maria
AU - de Souza, Evandro L.
PY - 2016
Y1 - 2016
N2 - This study aimed to identify lactic acid bacteria (LAB)
in byproducts of fruit (Malpighia glabra L., Mangifera
indica L., Annona muricata L., and Fragaria vesca L.)
pulp processing. Fifty strains of LAB were identified
using matrix-assisted laser desorption/ionization-time of
flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene
sequence (16S rRNA) analysis. Species belonging to
Lactobacillus genus were the predominant LAB in all fruit
pulp processing byproducts. The average congruency
between the MALDI-TOF MS and 16S rRNA in LAB species
identification reached 86%. Isolates of L. plantarum, L.
brevis, L. pentosus, L. lactis and L. mesenteroides were
identified with 100% congruency. MALDI-TOF MS and 16S
rRNA analysis presented 86 and 100% efficiency of LAB
species identification, respectively. Further, five
selected Lactobacillus strains (L. brevis 59, L. pentosus
129, L. paracasei 108, L. plantarum 49, and L. fermentum
111) were evaluated for desirable probiotic-related
properties and growth behavior on two different
cultivation media. The exposure to pH 2.0 sharply
decreased the counts of the different Lactobacillus
strains after a 1 or 2 h incubation, while varied
decreases were noted after 3 h of exposure to pH 3.0.
Overall, the exposure to pH 5.0 and to bile salts (0.15,
0.30, and 1.00%) did not decrease the counts of the
Lactobacillus strains. All tested Lactobacillus strains
presented inhibitory activity against Staphylococcus
aureus, Salmonella Typhimurium, Salmonella Enteritidis,
Listeria monocytogenes and Escherichia coli, and
presented variable susceptibility to different
antibiotics. The selected Lactobacillus strains presented
satisfactory and reproducible growth behavior. In
conclusion, MALDI-TOF MS and 16S rRNA analysis revealed
high efficiency and congruency for LAB species
identification, and the selected Lactobacillus strains
may be candidates for further investigation of novel
probiotic strains.
AB - This study aimed to identify lactic acid bacteria (LAB)
in byproducts of fruit (Malpighia glabra L., Mangifera
indica L., Annona muricata L., and Fragaria vesca L.)
pulp processing. Fifty strains of LAB were identified
using matrix-assisted laser desorption/ionization-time of
flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene
sequence (16S rRNA) analysis. Species belonging to
Lactobacillus genus were the predominant LAB in all fruit
pulp processing byproducts. The average congruency
between the MALDI-TOF MS and 16S rRNA in LAB species
identification reached 86%. Isolates of L. plantarum, L.
brevis, L. pentosus, L. lactis and L. mesenteroides were
identified with 100% congruency. MALDI-TOF MS and 16S
rRNA analysis presented 86 and 100% efficiency of LAB
species identification, respectively. Further, five
selected Lactobacillus strains (L. brevis 59, L. pentosus
129, L. paracasei 108, L. plantarum 49, and L. fermentum
111) were evaluated for desirable probiotic-related
properties and growth behavior on two different
cultivation media. The exposure to pH 2.0 sharply
decreased the counts of the different Lactobacillus
strains after a 1 or 2 h incubation, while varied
decreases were noted after 3 h of exposure to pH 3.0.
Overall, the exposure to pH 5.0 and to bile salts (0.15,
0.30, and 1.00%) did not decrease the counts of the
Lactobacillus strains. All tested Lactobacillus strains
presented inhibitory activity against Staphylococcus
aureus, Salmonella Typhimurium, Salmonella Enteritidis,
Listeria monocytogenes and Escherichia coli, and
presented variable susceptibility to different
antibiotics. The selected Lactobacillus strains presented
satisfactory and reproducible growth behavior. In
conclusion, MALDI-TOF MS and 16S rRNA analysis revealed
high efficiency and congruency for LAB species
identification, and the selected Lactobacillus strains
may be candidates for further investigation of novel
probiotic strains.
KW - 16S rRNA gene sequencing
KW - Fruit
KW - Lactobacillus
KW - MALDI-TOF profiling
KW - Probiotic
U2 - 10.3389/fmicb.2016.01371
DO - 10.3389/fmicb.2016.01371
M3 - Article
SN - 1664-302X
VL - 7
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 1371
ER -