Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact

Suvi-Katri Leivonen (Corresponding Author), Anne Rokka, Päivi Östling, Pekka Kohonen, Garry L. Corthals, Olli Kallioniemi, Merja Perälä

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Identification of protein targets for microRNAs (miRNAs) is a significant challenge due to the complexity of miRNA-mediated regulation. We have previously demonstrated that miR-193b targets estrogen receptor-α (ERα) and inhibits estrogen-induced growth of breast cancer cells. Here, we applied a high-throughput strategy using quantitative iTRAQ (isobaric tag for relative and absolute quantitation) reagents to identify other target proteins regulated by miR-193b in breast cancer cells.
Original languageEnglish
JournalMolecular and Cellular Proteomics
Volume10
Issue number7
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

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Biological systems
MicroRNAs
Cells
Breast Neoplasms
Estrogen Receptors
Estrogens
Proteins
Throughput
Growth

Cite this

Leivonen, Suvi-Katri ; Rokka, Anne ; Östling, Päivi ; Kohonen, Pekka ; Corthals, Garry L. ; Kallioniemi, Olli ; Perälä, Merja. / Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact. In: Molecular and Cellular Proteomics. 2011 ; Vol. 10, No. 7.
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Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact. / Leivonen, Suvi-Katri (Corresponding Author); Rokka, Anne; Östling, Päivi; Kohonen, Pekka; Corthals, Garry L.; Kallioniemi, Olli; Perälä, Merja.

In: Molecular and Cellular Proteomics, Vol. 10, No. 7, 2011.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Leivonen, Suvi-Katri

AU - Rokka, Anne

AU - Östling, Päivi

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AU - Corthals, Garry L.

AU - Kallioniemi, Olli

AU - Perälä, Merja

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AB - Identification of protein targets for microRNAs (miRNAs) is a significant challenge due to the complexity of miRNA-mediated regulation. We have previously demonstrated that miR-193b targets estrogen receptor-α (ERα) and inhibits estrogen-induced growth of breast cancer cells. Here, we applied a high-throughput strategy using quantitative iTRAQ (isobaric tag for relative and absolute quantitation) reagents to identify other target proteins regulated by miR-193b in breast cancer cells.

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JO - Molecular and Cellular Proteomics

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