Identification of protein targets for microRNAs (miRNAs) is a significant challenge due to the complexity of miRNA-mediated regulation. We have previously demonstrated that miR-193b targets estrogen receptor-α (ERα) and inhibits estrogen-induced growth of breast cancer cells. Here, we applied a high-throughput strategy using quantitative iTRAQ (isobaric tag for relative and absolute quantitation) reagents to identify other target proteins regulated by miR-193b in breast cancer cells.
Leivonen, S-K., Rokka, A., Östling, P., Kohonen, P., Corthals, G. L., Kallioniemi, O., & Perälä, M. (2011). Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact. Molecular and Cellular Proteomics, 10(7). https://doi.org/10.1074/mcp.M110.005322