Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact

Suvi-Katri Leivonen; Anne Rokka; Päivi Östling; Pekka Kohonen; Garry L. Corthals; Olli Kallioniemi; Merja Perälä

doi:10.1074/mcp.M110.005322

Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact

Suvi-Katri Leivonen^*
, Anne Rokka
, Päivi Östling
, Pekka Kohonen
, Garry L. Corthals
, Olli Kallioniemi
, Merja Perälä

^*Corresponding author for this work

VTT Technical Research Centre of Finland

VTT (former employee or external)
University of Turku
Åbo Akademi University
University of Helsinki

Research output: Contribution to journal › Article › Scientific › peer-review

65 Citations (Scopus)

Abstract

Identification of protein targets for microRNAs (miRNAs) is a significant challenge due to the complexity of miRNA-mediated regulation. We have previously demonstrated that miR-193b targets estrogen receptor-α (ERα) and inhibits estrogen-induced growth of breast cancer cells. Here, we applied a high-throughput strategy using quantitative iTRAQ (isobaric tag for relative and absolute quantitation) reagents to identify other target proteins regulated by miR-193b in breast cancer cells.

Original language	English
Journal	Molecular and Cellular Proteomics
Volume	10
Issue number	7
DOIs	https://doi.org/10.1074/mcp.M110.005322
Publication status	Published - 2011
MoE publication type	A1 Journal article-refereed

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SDG 3 Good Health and Well-being

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10.1074/mcp.M110.005322

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abstract = "Identification of protein targets for microRNAs (miRNAs) is a significant challenge due to the complexity of miRNA-mediated regulation. We have previously demonstrated that miR-193b targets estrogen receptor-α (ERα) and inhibits estrogen-induced growth of breast cancer cells. Here, we applied a high-throughput strategy using quantitative iTRAQ (isobaric tag for relative and absolute quantitation) reagents to identify other target proteins regulated by miR-193b in breast cancer cells.",

author = "Suvi-Katri Leivonen and Anne Rokka and P{\"a}ivi {\"O}stling and Pekka Kohonen and Corthals, \{Garry L.\} and Olli Kallioniemi and Merja Per{\"a}l{\"a}",

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AU - Leivonen, Suvi-Katri

AU - Rokka, Anne

AU - Östling, Päivi

AU - Kohonen, Pekka

AU - Corthals, Garry L.

AU - Kallioniemi, Olli

AU - Perälä, Merja

PY - 2011

Y1 - 2011

N2 - Identification of protein targets for microRNAs (miRNAs) is a significant challenge due to the complexity of miRNA-mediated regulation. We have previously demonstrated that miR-193b targets estrogen receptor-α (ERα) and inhibits estrogen-induced growth of breast cancer cells. Here, we applied a high-throughput strategy using quantitative iTRAQ (isobaric tag for relative and absolute quantitation) reagents to identify other target proteins regulated by miR-193b in breast cancer cells.

AB - Identification of protein targets for microRNAs (miRNAs) is a significant challenge due to the complexity of miRNA-mediated regulation. We have previously demonstrated that miR-193b targets estrogen receptor-α (ERα) and inhibits estrogen-induced growth of breast cancer cells. Here, we applied a high-throughput strategy using quantitative iTRAQ (isobaric tag for relative and absolute quantitation) reagents to identify other target proteins regulated by miR-193b in breast cancer cells.

U2 - 10.1074/mcp.M110.005322

DO - 10.1074/mcp.M110.005322

M3 - Article

SN - 1535-9476

VL - 10

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

IS - 7

ER -

Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact

Abstract

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