Identification of novel isoprene synthases through genome mining and expression in Escherichia coli

Marja Ilmén, Merja Oja, Anne Huuskonen, Sangmin Lee, Laura Ruohonen, Simon Jung (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

Isoprene is a naturally produced hydrocarbon emitted into the atmosphere by green plants. It is also a constituent of synthetic rubber and a potential biofuel. Microbial production of isoprene can become a sustainable alternative to the prevailing chemical production of isoprene from petroleum. In this work, sequence homology searches were conducted to find novel isoprene synthases. Candidate sequences were functionally expressed in Escherichia coli and the desired enzymes were identified based on an isoprene production assay. The activity of three enzymes was shown for the first time: expression of the candidate genes from Ipomoea batatas, Mangifera indica, and Elaeocarpus photiniifolius resulted in isoprene formation. The Ipomoea batatas isoprene synthase produced the highest amounts of isoprene in all experiments, exceeding the isoprene levels obtained by the previously known Populus alba and Pueraria montana isoprene synthases that were studied in parallel as controls.
Original languageEnglish
Pages (from-to)153 -162
JournalMetabolic Engineering
Volume31
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Isoprene
Escherichia coli
Genes
Genome
Ipomoea batatas
Elaeocarpaceae
Pueraria
Viridiplantae
Elastomers
Populus
Mangifera
Biofuels
Petroleum
Enzymes
Sequence Homology
Hydrocarbons
isoprene
isoprene synthase
Atmosphere
Synthetic rubber

Keywords

  • isoprenoid
  • terpene
  • genome mining
  • homology-based screening

Cite this

Ilmén, Marja ; Oja, Merja ; Huuskonen, Anne ; Lee, Sangmin ; Ruohonen, Laura ; Jung, Simon. / Identification of novel isoprene synthases through genome mining and expression in Escherichia coli. In: Metabolic Engineering. 2015 ; Vol. 31. pp. 153 -162.
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Identification of novel isoprene synthases through genome mining and expression in Escherichia coli. / Ilmén, Marja; Oja, Merja; Huuskonen, Anne; Lee, Sangmin; Ruohonen, Laura; Jung, Simon (Corresponding Author).

In: Metabolic Engineering, Vol. 31, 2015, p. 153 -162.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Identification of novel isoprene synthases through genome mining and expression in Escherichia coli

AU - Ilmén, Marja

AU - Oja, Merja

AU - Huuskonen, Anne

AU - Lee, Sangmin

AU - Ruohonen, Laura

AU - Jung, Simon

PY - 2015

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N2 - Isoprene is a naturally produced hydrocarbon emitted into the atmosphere by green plants. It is also a constituent of synthetic rubber and a potential biofuel. Microbial production of isoprene can become a sustainable alternative to the prevailing chemical production of isoprene from petroleum. In this work, sequence homology searches were conducted to find novel isoprene synthases. Candidate sequences were functionally expressed in Escherichia coli and the desired enzymes were identified based on an isoprene production assay. The activity of three enzymes was shown for the first time: expression of the candidate genes from Ipomoea batatas, Mangifera indica, and Elaeocarpus photiniifolius resulted in isoprene formation. The Ipomoea batatas isoprene synthase produced the highest amounts of isoprene in all experiments, exceeding the isoprene levels obtained by the previously known Populus alba and Pueraria montana isoprene synthases that were studied in parallel as controls.

AB - Isoprene is a naturally produced hydrocarbon emitted into the atmosphere by green plants. It is also a constituent of synthetic rubber and a potential biofuel. Microbial production of isoprene can become a sustainable alternative to the prevailing chemical production of isoprene from petroleum. In this work, sequence homology searches were conducted to find novel isoprene synthases. Candidate sequences were functionally expressed in Escherichia coli and the desired enzymes were identified based on an isoprene production assay. The activity of three enzymes was shown for the first time: expression of the candidate genes from Ipomoea batatas, Mangifera indica, and Elaeocarpus photiniifolius resulted in isoprene formation. The Ipomoea batatas isoprene synthase produced the highest amounts of isoprene in all experiments, exceeding the isoprene levels obtained by the previously known Populus alba and Pueraria montana isoprene synthases that were studied in parallel as controls.

KW - isoprenoid

KW - terpene

KW - genome mining

KW - homology-based screening

U2 - 10.1016/j.ymben.2015.08.001

DO - 10.1016/j.ymben.2015.08.001

M3 - Article

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JO - Metabolic Engineering

JF - Metabolic Engineering

SN - 1096-7176

ER -