Identification of nucleolar effects in JNK-deficient cells

Antoine Mialon, Jacob Thastrup, Tuula Kallunki, Leni Mannermaa, Jukka Westermarck, Tim H. Holmström (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

The c‐Jun N‐terminal kinase (JNK) signalling pathway has an established role in cellular stress signalling, cell survival and tumorigenesis. Here, we demonstrate that inhibition of JNK signalling results in partial delocalization of the RNA helicase DDX21 from the nucleolus to the nucleoplasm, increased nucleolar mobility of DDX21 and inhibition of rRNA processing. Furthermore, our results show that JNK signalling regulates DDX21 phosphorylation and protein expression. In conclusion, the results presented in this study reveal a previously unidentified cellular role for JNK signalling in the regulation of nucleolar functions. Based on these results, we propose that JNK‐mediated effects on nucleolar homeostasis and rRNA processing should be considered when interpreting cellular phenotypes observed in JNK‐deficient cell and animal models.
Original languageEnglish
Pages (from-to)3145-3151
JournalFEBS Letters
Volume582
Issue number20
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

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RNA Helicases
MAP Kinase Signaling System
Cell Survival
Carcinogenesis
Homeostasis
Phosphotransferases
Animal Models
Phosphorylation
Phenotype
Proteins
MAP Kinase Kinase 4
Cell signaling
Processing
Animals

Keywords

  • DDX21
  • JNK
  • Kinase
  • Nucleolus
  • Phosphorylation
  • RNA helicase

Cite this

Mialon, A., Thastrup, J., Kallunki, T., Mannermaa, L., Westermarck, J., & Holmström, T. H. (2008). Identification of nucleolar effects in JNK-deficient cells. FEBS Letters, 582(20), 3145-3151. https://doi.org/10.1016/j.febslet.2008.08.004
Mialon, Antoine ; Thastrup, Jacob ; Kallunki, Tuula ; Mannermaa, Leni ; Westermarck, Jukka ; Holmström, Tim H. / Identification of nucleolar effects in JNK-deficient cells. In: FEBS Letters. 2008 ; Vol. 582, No. 20. pp. 3145-3151.
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Mialon, A, Thastrup, J, Kallunki, T, Mannermaa, L, Westermarck, J & Holmström, TH 2008, 'Identification of nucleolar effects in JNK-deficient cells', FEBS Letters, vol. 582, no. 20, pp. 3145-3151. https://doi.org/10.1016/j.febslet.2008.08.004

Identification of nucleolar effects in JNK-deficient cells. / Mialon, Antoine; Thastrup, Jacob; Kallunki, Tuula; Mannermaa, Leni; Westermarck, Jukka; Holmström, Tim H. (Corresponding Author).

In: FEBS Letters, Vol. 582, No. 20, 2008, p. 3145-3151.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Identification of nucleolar effects in JNK-deficient cells

AU - Mialon, Antoine

AU - Thastrup, Jacob

AU - Kallunki, Tuula

AU - Mannermaa, Leni

AU - Westermarck, Jukka

AU - Holmström, Tim H.

PY - 2008

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N2 - The c‐Jun N‐terminal kinase (JNK) signalling pathway has an established role in cellular stress signalling, cell survival and tumorigenesis. Here, we demonstrate that inhibition of JNK signalling results in partial delocalization of the RNA helicase DDX21 from the nucleolus to the nucleoplasm, increased nucleolar mobility of DDX21 and inhibition of rRNA processing. Furthermore, our results show that JNK signalling regulates DDX21 phosphorylation and protein expression. In conclusion, the results presented in this study reveal a previously unidentified cellular role for JNK signalling in the regulation of nucleolar functions. Based on these results, we propose that JNK‐mediated effects on nucleolar homeostasis and rRNA processing should be considered when interpreting cellular phenotypes observed in JNK‐deficient cell and animal models.

AB - The c‐Jun N‐terminal kinase (JNK) signalling pathway has an established role in cellular stress signalling, cell survival and tumorigenesis. Here, we demonstrate that inhibition of JNK signalling results in partial delocalization of the RNA helicase DDX21 from the nucleolus to the nucleoplasm, increased nucleolar mobility of DDX21 and inhibition of rRNA processing. Furthermore, our results show that JNK signalling regulates DDX21 phosphorylation and protein expression. In conclusion, the results presented in this study reveal a previously unidentified cellular role for JNK signalling in the regulation of nucleolar functions. Based on these results, we propose that JNK‐mediated effects on nucleolar homeostasis and rRNA processing should be considered when interpreting cellular phenotypes observed in JNK‐deficient cell and animal models.

KW - DDX21

KW - JNK

KW - Kinase

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KW - Phosphorylation

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Mialon A, Thastrup J, Kallunki T, Mannermaa L, Westermarck J, Holmström TH. Identification of nucleolar effects in JNK-deficient cells. FEBS Letters. 2008;582(20):3145-3151. https://doi.org/10.1016/j.febslet.2008.08.004