Identification of Shiitake strains and some other basidiomycetes: Protein profile, esterase and acid phosphatase zymograms as an aid in taxonomy

Merja Itävaara

Research output: Contribution to journalArticleScientific

Abstract

Lentinula edodes strains exhibited a typical total protein pattern when native soluble proteins were separated by isoelectric focusing at a pH gradient of 3·5–10. Intraspecies variation was found when using a narrower acidic pH gradient of 4–6. Esterase zymograms showed variation between the shiitake strains. However, the variation was wider between different wood-rotting species.

Although the acid phosphatase isoenzyme patterns were all similar in the shiitake strains, variation was observed between the different basidiomycete species. The use of acid phosphatase and esterase as an aid in the taxonomy of wood rotting basidiomycetes is discussed.
Original languageEnglish
Pages (from-to)295-304
JournalTransactions of the British Mycological Society
Volume91
Issue number2
DOIs
Publication statusPublished - 1988
MoE publication typeB1 Article in a scientific magazine

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