Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 197-208 |
| Number of pages | 12 |
| Journal | Carbohydrate Research |
| Volume | 280 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1996 |
| MoE publication type | A1 Journal article-refereed |
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Identification of the acidic degradation products of hexenuronic acid and characterisation of hexenuronic acid-substituted xylooligosaccharides by NMR spectroscopy. / Teleman, Anita (Corresponding Author); Hausalo, Tiina; Tenkanen, Maija; Vuorinen, Tapani.
In: Carbohydrate Research, Vol. 280, No. 2, 1996, p. 197-208.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Identification of the acidic degradation products of hexenuronic acid and characterisation of hexenuronic acid-substituted xylooligosaccharides by NMR spectroscopy
AU - Teleman, Anita
AU - Hausalo, Tiina
AU - Tenkanen, Maija
AU - Vuorinen, Tapani
PY - 1996
Y1 - 1996
N2 - A 4-O-methylglucuronoxylan was converted into a hexenuronoxylan at high temperature and alkalinity similar to the conditions used during kraft pulping. The hexenuronoxylan was hydrolysed with enzymes, and acidic xylooligosaccharides were separated from the hydrolysate by anion-exchange and size-exclusion chromatography. The primary structure of the two main hexenuronic acid-substituted xylooligosaccharides (a tetramer and a pentamer) was determined by two-dimensional 1H and 13C NMR spectroscopy. The 4-deoxy-hexenutronic acid is not stable under the acid hydrolysis step of conventional carbohydrate analysis. Here, we have identified the acidic degradation products of 4-deoxy-hexenuronic acid by NMR spectroscopy. Two degradation pathways were observed, both resulting in a furan derivative.
AB - A 4-O-methylglucuronoxylan was converted into a hexenuronoxylan at high temperature and alkalinity similar to the conditions used during kraft pulping. The hexenuronoxylan was hydrolysed with enzymes, and acidic xylooligosaccharides were separated from the hydrolysate by anion-exchange and size-exclusion chromatography. The primary structure of the two main hexenuronic acid-substituted xylooligosaccharides (a tetramer and a pentamer) was determined by two-dimensional 1H and 13C NMR spectroscopy. The 4-deoxy-hexenutronic acid is not stable under the acid hydrolysis step of conventional carbohydrate analysis. Here, we have identified the acidic degradation products of 4-deoxy-hexenuronic acid by NMR spectroscopy. Two degradation pathways were observed, both resulting in a furan derivative.
U2 - 10.1016/0008-6215(95)00309-6
DO - 10.1016/0008-6215(95)00309-6
M3 - Article
VL - 280
SP - 197
EP - 208
JO - Carbohydrate Research
JF - Carbohydrate Research
SN - 0008-6215
IS - 2
ER -