Abstract
Thermophilic acetate-utilizing bacteria were isolated by using in situ enrichment methods. The bacteria were enriched in a hot spring by feeding 3 mol/l ammonium acetate or 3 mol/l ammonium acetate with 0.5 mol/l sodium sulfite semi-continuously into the hot spring for several weeks. The bacteria were then isolated in the laboratory by conventional enrichment and plating techniques. Bacteria were also isolated from a wood pulping mill. The isolates could grow at 65 °C on 12.8–18 g/l of ammonium acetate. Some strains grew even with 2.5 g/l of sodium sulfite added, as well as in media made out of evaporator condensate produced at a pulp mill. All isolates which grew in the highest concentrations of ammonium acetate were found to be Gram-positive, sporeforming, alkalophilic bacilli.
| Original language | English |
|---|---|
| Pages (from-to) | 190 - 195 |
| Number of pages | 6 |
| Journal | Systematic and Applied Microbiology |
| Volume | 14 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1991 |
| MoE publication type | A1 Journal article-refereed |
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In situ enrichment of thermophilic acetate-utilizing bacteria. / Konradsdottir, Marta; Perttula, Martti; Pere, Jaakko; Viikari, Liisa; Kristjansson, Jakob (Corresponding Author).
In: Systematic and Applied Microbiology, Vol. 14, No. 2, 1991, p. 190 - 195.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - In situ enrichment of thermophilic acetate-utilizing bacteria
AU - Konradsdottir, Marta
AU - Perttula, Martti
AU - Pere, Jaakko
AU - Viikari, Liisa
AU - Kristjansson, Jakob
N1 - Project code: BIO0110
PY - 1991
Y1 - 1991
N2 - Thermophilic acetate-utilizing bacteria were isolated by using in situ enrichment methods. The bacteria were enriched in a hot spring by feeding 3 mol/l ammonium acetate or 3 mol/l ammonium acetate with 0.5 mol/l sodium sulfite semi-continuously into the hot spring for several weeks. The bacteria were then isolated in the laboratory by conventional enrichment and plating techniques. Bacteria were also isolated from a wood pulping mill. The isolates could grow at 65 °C on 12.8–18 g/l of ammonium acetate. Some strains grew even with 2.5 g/l of sodium sulfite added, as well as in media made out of evaporator condensate produced at a pulp mill. All isolates which grew in the highest concentrations of ammonium acetate were found to be Gram-positive, sporeforming, alkalophilic bacilli.
AB - Thermophilic acetate-utilizing bacteria were isolated by using in situ enrichment methods. The bacteria were enriched in a hot spring by feeding 3 mol/l ammonium acetate or 3 mol/l ammonium acetate with 0.5 mol/l sodium sulfite semi-continuously into the hot spring for several weeks. The bacteria were then isolated in the laboratory by conventional enrichment and plating techniques. Bacteria were also isolated from a wood pulping mill. The isolates could grow at 65 °C on 12.8–18 g/l of ammonium acetate. Some strains grew even with 2.5 g/l of sodium sulfite added, as well as in media made out of evaporator condensate produced at a pulp mill. All isolates which grew in the highest concentrations of ammonium acetate were found to be Gram-positive, sporeforming, alkalophilic bacilli.
U2 - 10.1016/S0723-2020(11)80301-2
DO - 10.1016/S0723-2020(11)80301-2
M3 - Article
VL - 14
SP - 190
EP - 195
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
SN - 0723-2020
IS - 2
ER -