TY - JOUR
T1 - In situ enrichment of thermophilic acetate-utilizing bacteria
AU - Konradsdottir, Marta
AU - Perttula, Martti
AU - Pere, Jaakko
AU - Viikari, Liisa
AU - Kristjansson, Jakob
N1 - Project code: BIO0110
PY - 1991
Y1 - 1991
N2 - Thermophilic acetate-utilizing bacteria were isolated by using in situ
enrichment methods. The bacteria were enriched in a hot spring by
feeding 3 mol/l ammonium acetate or 3 mol/l ammonium acetate with 0.5
mol/l sodium sulfite semi-continuously into the hot spring for several
weeks. The bacteria were then isolated in the laboratory by conventional
enrichment and plating techniques. Bacteria were also isolated from a
wood pulping mill. The isolates could grow at 65 °C on 12.8–18 g/l of
ammonium acetate. Some strains grew even with 2.5 g/l of sodium sulfite
added, as well as in media made out of evaporator condensate produced at
a pulp mill. All isolates which grew in the highest concentrations of
ammonium acetate were found to be Gram-positive, sporeforming,
alkalophilic bacilli.
AB - Thermophilic acetate-utilizing bacteria were isolated by using in situ
enrichment methods. The bacteria were enriched in a hot spring by
feeding 3 mol/l ammonium acetate or 3 mol/l ammonium acetate with 0.5
mol/l sodium sulfite semi-continuously into the hot spring for several
weeks. The bacteria were then isolated in the laboratory by conventional
enrichment and plating techniques. Bacteria were also isolated from a
wood pulping mill. The isolates could grow at 65 °C on 12.8–18 g/l of
ammonium acetate. Some strains grew even with 2.5 g/l of sodium sulfite
added, as well as in media made out of evaporator condensate produced at
a pulp mill. All isolates which grew in the highest concentrations of
ammonium acetate were found to be Gram-positive, sporeforming,
alkalophilic bacilli.
U2 - 10.1016/S0723-2020(11)80301-2
DO - 10.1016/S0723-2020(11)80301-2
M3 - Article
SN - 0723-2020
VL - 14
SP - 190
EP - 195
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 2
ER -