In situ enrichment of thermophilic acetate-utilizing bacteria

Marta Konradsdottir; Martti Perttula; Jaakko Pere; Liisa Viikari; Jakob Kristjansson

doi:10.1016/S0723-2020(11)80301-2

In situ enrichment of thermophilic acetate-utilizing bacteria

Marta Konradsdottir, Martti Perttula, Jaakko Pere, Liisa Viikari, Jakob Kristjansson (Corresponding Author)

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Abstract

Thermophilic acetate-utilizing bacteria were isolated by using in situ enrichment methods. The bacteria were enriched in a hot spring by feeding 3 mol/l ammonium acetate or 3 mol/l ammonium acetate with 0.5 mol/l sodium sulfite semi-continuously into the hot spring for several weeks. The bacteria were then isolated in the laboratory by conventional enrichment and plating techniques. Bacteria were also isolated from a wood pulping mill. The isolates could grow at 65 °C on 12.8–18 g/l of ammonium acetate. Some strains grew even with 2.5 g/l of sodium sulfite added, as well as in media made out of evaporator condensate produced at a pulp mill. All isolates which grew in the highest concentrations of ammonium acetate were found to be Gram-positive, sporeforming, alkalophilic bacilli.

Original language	English
Pages (from-to)	190 - 195
Number of pages	6
Journal	Systematic and Applied Microbiology
Volume	14
Issue number	2
DOIs	https://doi.org/10.1016/S0723-2020(11)80301-2
Publication status	Published - 1991
MoE publication type	A1 Journal article-refereed

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title = "In situ enrichment of thermophilic acetate-utilizing bacteria",

abstract = "Thermophilic acetate-utilizing bacteria were isolated by using in situ enrichment methods. The bacteria were enriched in a hot spring by feeding 3 mol/l ammonium acetate or 3 mol/l ammonium acetate with 0.5 mol/l sodium sulfite semi-continuously into the hot spring for several weeks. The bacteria were then isolated in the laboratory by conventional enrichment and plating techniques. Bacteria were also isolated from a wood pulping mill. The isolates could grow at 65 °C on 12.8–18 g/l of ammonium acetate. Some strains grew even with 2.5 g/l of sodium sulfite added, as well as in media made out of evaporator condensate produced at a pulp mill. All isolates which grew in the highest concentrations of ammonium acetate were found to be Gram-positive, sporeforming, alkalophilic bacilli.",

author = "Marta Konradsdottir and Martti Perttula and Jaakko Pere and Liisa Viikari and Jakob Kristjansson",

note = "Project code: BIO0110",

year = "1991",

doi = "10.1016/S0723-2020(11)80301-2",

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volume = "14",

pages = "190 -- 195",

journal = "Systematic and Applied Microbiology",

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publisher = "Urban & Fischer",

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T1 - In situ enrichment of thermophilic acetate-utilizing bacteria

AU - Konradsdottir, Marta

AU - Perttula, Martti

AU - Pere, Jaakko

AU - Viikari, Liisa

AU - Kristjansson, Jakob

N1 - Project code: BIO0110

PY - 1991

Y1 - 1991

N2 - Thermophilic acetate-utilizing bacteria were isolated by using in situ enrichment methods. The bacteria were enriched in a hot spring by feeding 3 mol/l ammonium acetate or 3 mol/l ammonium acetate with 0.5 mol/l sodium sulfite semi-continuously into the hot spring for several weeks. The bacteria were then isolated in the laboratory by conventional enrichment and plating techniques. Bacteria were also isolated from a wood pulping mill. The isolates could grow at 65 °C on 12.8–18 g/l of ammonium acetate. Some strains grew even with 2.5 g/l of sodium sulfite added, as well as in media made out of evaporator condensate produced at a pulp mill. All isolates which grew in the highest concentrations of ammonium acetate were found to be Gram-positive, sporeforming, alkalophilic bacilli.

AB - Thermophilic acetate-utilizing bacteria were isolated by using in situ enrichment methods. The bacteria were enriched in a hot spring by feeding 3 mol/l ammonium acetate or 3 mol/l ammonium acetate with 0.5 mol/l sodium sulfite semi-continuously into the hot spring for several weeks. The bacteria were then isolated in the laboratory by conventional enrichment and plating techniques. Bacteria were also isolated from a wood pulping mill. The isolates could grow at 65 °C on 12.8–18 g/l of ammonium acetate. Some strains grew even with 2.5 g/l of sodium sulfite added, as well as in media made out of evaporator condensate produced at a pulp mill. All isolates which grew in the highest concentrations of ammonium acetate were found to be Gram-positive, sporeforming, alkalophilic bacilli.

U2 - 10.1016/S0723-2020(11)80301-2

DO - 10.1016/S0723-2020(11)80301-2

M3 - Article

VL - 14

SP - 190

EP - 195

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

SN - 0723-2020

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