In situ enrichment of thermophilic acetate-utilizing bacteria

Marta Konradsdottir, Martti Perttula, Jaakko Pere, Liisa Viikari, Jakob Kristjansson (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Thermophilic acetate-utilizing bacteria were isolated by using in situ enrichment methods. The bacteria were enriched in a hot spring by feeding 3 mol/l ammonium acetate or 3 mol/l ammonium acetate with 0.5 mol/l sodium sulfite semi-continuously into the hot spring for several weeks. The bacteria were then isolated in the laboratory by conventional enrichment and plating techniques. Bacteria were also isolated from a wood pulping mill. The isolates could grow at 65 °C on 12.8–18 g/l of ammonium acetate. Some strains grew even with 2.5 g/l of sodium sulfite added, as well as in media made out of evaporator condensate produced at a pulp mill. All isolates which grew in the highest concentrations of ammonium acetate were found to be Gram-positive, sporeforming, alkalophilic bacilli.

Original languageEnglish
Pages (from-to)190 - 195
Number of pages6
JournalSystematic and Applied Microbiology
Volume14
Issue number2
DOIs
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

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ammonium acetate
acetate
Acetates
acetates
Hot Springs
sodium sulfite
Bacteria
ammonium
hot springs
bacterium
bacteria
sulfite
thermal spring
mill
pulping
evaporators
sodium
Bacillus
pulp
condensate

Cite this

Konradsdottir, Marta ; Perttula, Martti ; Pere, Jaakko ; Viikari, Liisa ; Kristjansson, Jakob. / In situ enrichment of thermophilic acetate-utilizing bacteria. In: Systematic and Applied Microbiology. 1991 ; Vol. 14, No. 2. pp. 190 - 195.
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abstract = "Thermophilic acetate-utilizing bacteria were isolated by using in situ enrichment methods. The bacteria were enriched in a hot spring by feeding 3 mol/l ammonium acetate or 3 mol/l ammonium acetate with 0.5 mol/l sodium sulfite semi-continuously into the hot spring for several weeks. The bacteria were then isolated in the laboratory by conventional enrichment and plating techniques. Bacteria were also isolated from a wood pulping mill. The isolates could grow at 65 °C on 12.8–18 g/l of ammonium acetate. Some strains grew even with 2.5 g/l of sodium sulfite added, as well as in media made out of evaporator condensate produced at a pulp mill. All isolates which grew in the highest concentrations of ammonium acetate were found to be Gram-positive, sporeforming, alkalophilic bacilli.",
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Konradsdottir, M, Perttula, M, Pere, J, Viikari, L & Kristjansson, J 1991, 'In situ enrichment of thermophilic acetate-utilizing bacteria', Systematic and Applied Microbiology, vol. 14, no. 2, pp. 190 - 195. https://doi.org/10.1016/S0723-2020(11)80301-2

In situ enrichment of thermophilic acetate-utilizing bacteria. / Konradsdottir, Marta; Perttula, Martti; Pere, Jaakko; Viikari, Liisa; Kristjansson, Jakob (Corresponding Author).

In: Systematic and Applied Microbiology, Vol. 14, No. 2, 1991, p. 190 - 195.

Research output: Contribution to journalArticleScientificpeer-review

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