In vitro digestion of wheat microstructure with xylanase and cellulase from Trichoderma reesei

Asta Tervilä-Wilo, Teija Parkkonen, Andrew Morgan, Maarit Hopeakoski-Nurminen, Kaisa Poutanen, P. Heikkinen, Karin Autio

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

A three-stage in vitro model was used to simulate the digestion of wheat in the broiler chicken. During digestion simulation, much of the wheat protein was liberated into the supernatant. Microscopic examination of the residue showed that protein could be detected only within unbroken cells. Intact starch granules were clearly visible following digestion simulation and only a small amount (ca. 8%) of glucose was released into the supernatant. The effects of enzymes from Trichoderma reesei on wheat cell structure and the release of protein and non-starch carbohydrate were studied. Addition of cell wall degrading enzymes increased the release of proteins and non-starch carbohydrate. The hydrolysis of wheat cell walls increased with concentrations of cell wall degrading enzymes. Microscopy results were consistent with those from chemical analysis.
Original languageEnglish
Pages (from-to)215-225
Number of pages11
JournalJournal of Cereal Science
Volume24
Issue number3
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma reesei
Trichoderma
in vitro digestion
Cellulase
xylanases
endo-1,4-beta-glucanase
microstructure
Triticum
Digestion
digestion
cell walls
Cell Wall
Microstructure
wheat
Cells
enzymes
carbohydrates
Microscopic examination
Proteins
wheat protein

Cite this

Tervilä-Wilo, A., Parkkonen, T., Morgan, A., Hopeakoski-Nurminen, M., Poutanen, K., Heikkinen, P., & Autio, K. (1996). In vitro digestion of wheat microstructure with xylanase and cellulase from Trichoderma reesei. Journal of Cereal Science, 24(3), 215-225. https://doi.org/10.1006/jcrs.1996.0054
Tervilä-Wilo, Asta ; Parkkonen, Teija ; Morgan, Andrew ; Hopeakoski-Nurminen, Maarit ; Poutanen, Kaisa ; Heikkinen, P. ; Autio, Karin. / In vitro digestion of wheat microstructure with xylanase and cellulase from Trichoderma reesei. In: Journal of Cereal Science. 1996 ; Vol. 24, No. 3. pp. 215-225.
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abstract = "A three-stage in vitro model was used to simulate the digestion of wheat in the broiler chicken. During digestion simulation, much of the wheat protein was liberated into the supernatant. Microscopic examination of the residue showed that protein could be detected only within unbroken cells. Intact starch granules were clearly visible following digestion simulation and only a small amount (ca. 8{\%}) of glucose was released into the supernatant. The effects of enzymes from Trichoderma reesei on wheat cell structure and the release of protein and non-starch carbohydrate were studied. Addition of cell wall degrading enzymes increased the release of proteins and non-starch carbohydrate. The hydrolysis of wheat cell walls increased with concentrations of cell wall degrading enzymes. Microscopy results were consistent with those from chemical analysis.",
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Tervilä-Wilo, A, Parkkonen, T, Morgan, A, Hopeakoski-Nurminen, M, Poutanen, K, Heikkinen, P & Autio, K 1996, 'In vitro digestion of wheat microstructure with xylanase and cellulase from Trichoderma reesei', Journal of Cereal Science, vol. 24, no. 3, pp. 215-225. https://doi.org/10.1006/jcrs.1996.0054

In vitro digestion of wheat microstructure with xylanase and cellulase from Trichoderma reesei. / Tervilä-Wilo, Asta; Parkkonen, Teija; Morgan, Andrew; Hopeakoski-Nurminen, Maarit; Poutanen, Kaisa; Heikkinen, P.; Autio, Karin.

In: Journal of Cereal Science, Vol. 24, No. 3, 1996, p. 215-225.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - In vitro digestion of wheat microstructure with xylanase and cellulase from Trichoderma reesei

AU - Tervilä-Wilo, Asta

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AU - Morgan, Andrew

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AU - Poutanen, Kaisa

AU - Heikkinen, P.

AU - Autio, Karin

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