In vitro models for studying secondary plant metabolite digestion and bioaccessibility

M. Alminger, Anna-Marja Aura, T. Bohn, C. Dufour, S.N. El, A. Gomes, S. Karakaya, M.C. Martinez-Cuesta, G.J. Mcdougall, T. Requena, C.N. Santos (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

108 Citations (Scopus)

Abstract

There is an increased interest in secondary plant metabolites, such as polyphenols and carotenoids, due to their proposed health benefits. Much attention has focused on their bioavailability, a prerequisite for further physiological functions. As human studies are time consuming, costly, and restricted by ethical concerns, in vitro models for investigating the effects of digestion on these compounds have been developed and employed to predict their release from the food matrix, bioaccessibility, and assess changes in their profiles prior to absorption. Most typically, models simulate digestion in the oral cavity, the stomach, the small intestine, and, occasionally, the large intestine. A plethora of models have been reported, the choice mostly driven by the type of phytochemical studied, whether the purpose is screening or studying under close physiological conditions, and the availability of the model systems. Unfortunately, the diversity of model conditions has hampered the ability to compare results across different studies. For example, there is substantial variability in the time of digestion, concentrations of salts, enzymes, and bile acids used, pH, the inclusion of various digestion stages; and whether chosen conditions are static (with fixed concentrations of enzymes, bile salts, digesta, and so on) or dynamic (varying concentrations of these constituents). This review presents an overview of models that have been employed to study the digestion of both lipophilic and hydrophilic phytochemicals, comparing digestive conditions in vitro and in vivo and, finally, suggests a set of parameters for static models that resemble physiological conditions.
Original languageEnglish
Pages (from-to)413-436
Number of pages23
JournalComprehensive Reviews in Food Science and Food Safety
Volume13
Issue number4
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Digestion
digestion
metabolites
Phytochemicals
Bile Acids and Salts
phytopharmaceuticals
Large Intestine
Polyphenols
Insurance Benefits
Enzymes
Carotenoids
Biological Availability
Small Intestine
Mouth
bile salts
Stomach
food matrix
bile acids
large intestine
digesta

Keywords

  • phytochemicals
  • carotenoids
  • polyphenols
  • gastrointestinal digestion
  • in vitro models
  • bioaccessibility

Cite this

Alminger, M. ; Aura, Anna-Marja ; Bohn, T. ; Dufour, C. ; El, S.N. ; Gomes, A. ; Karakaya, S. ; Martinez-Cuesta, M.C. ; Mcdougall, G.J. ; Requena, T. ; Santos, C.N. / In vitro models for studying secondary plant metabolite digestion and bioaccessibility. In: Comprehensive Reviews in Food Science and Food Safety. 2014 ; Vol. 13, No. 4. pp. 413-436.
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Alminger, M, Aura, A-M, Bohn, T, Dufour, C, El, SN, Gomes, A, Karakaya, S, Martinez-Cuesta, MC, Mcdougall, GJ, Requena, T & Santos, CN 2014, 'In vitro models for studying secondary plant metabolite digestion and bioaccessibility', Comprehensive Reviews in Food Science and Food Safety, vol. 13, no. 4, pp. 413-436. https://doi.org/10.1111/1541-4337.12081

In vitro models for studying secondary plant metabolite digestion and bioaccessibility. / Alminger, M.; Aura, Anna-Marja; Bohn, T.; Dufour, C.; El, S.N.; Gomes, A.; Karakaya, S.; Martinez-Cuesta, M.C.; Mcdougall, G.J.; Requena, T.; Santos, C.N. (Corresponding Author).

In: Comprehensive Reviews in Food Science and Food Safety, Vol. 13, No. 4, 2014, p. 413-436.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Alminger, M.

AU - Aura, Anna-Marja

AU - Bohn, T.

AU - Dufour, C.

AU - El, S.N.

AU - Gomes, A.

AU - Karakaya, S.

AU - Martinez-Cuesta, M.C.

AU - Mcdougall, G.J.

AU - Requena, T.

AU - Santos, C.N.

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AB - There is an increased interest in secondary plant metabolites, such as polyphenols and carotenoids, due to their proposed health benefits. Much attention has focused on their bioavailability, a prerequisite for further physiological functions. As human studies are time consuming, costly, and restricted by ethical concerns, in vitro models for investigating the effects of digestion on these compounds have been developed and employed to predict their release from the food matrix, bioaccessibility, and assess changes in their profiles prior to absorption. Most typically, models simulate digestion in the oral cavity, the stomach, the small intestine, and, occasionally, the large intestine. A plethora of models have been reported, the choice mostly driven by the type of phytochemical studied, whether the purpose is screening or studying under close physiological conditions, and the availability of the model systems. Unfortunately, the diversity of model conditions has hampered the ability to compare results across different studies. For example, there is substantial variability in the time of digestion, concentrations of salts, enzymes, and bile acids used, pH, the inclusion of various digestion stages; and whether chosen conditions are static (with fixed concentrations of enzymes, bile salts, digesta, and so on) or dynamic (varying concentrations of these constituents). This review presents an overview of models that have been employed to study the digestion of both lipophilic and hydrophilic phytochemicals, comparing digestive conditions in vitro and in vivo and, finally, suggests a set of parameters for static models that resemble physiological conditions.

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KW - carotenoids

KW - polyphenols

KW - gastrointestinal digestion

KW - in vitro models

KW - bioaccessibility

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DO - 10.1111/1541-4337.12081

M3 - Article

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JO - Comprehensive Reviews in Food Science and Food Safety

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