In vivo FLIM-FRET measurements of recombinant proteins expressed in filamentous fungi

K. Altenbach, R. R. Duncan, Mari Valkonen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

Fluorescence imaging techniques are extremely powerful tools in cell biology, providing valuable insights into the structure and function of biomolecules in their native environments. In particular, the use of Förster resonance energy transfer (FRET) has become increasingly important to obtain information on interactions on the nanoscale, in turn providing insights into molecular behaviour inside living cells. This review describes the basic principles of FRET and fluorescence lifetime imaging microscopy (FLIM) and their application in analyses of protein interactions inside living fungal cells.
Original languageEnglish
Pages (from-to)67-71
Number of pages5
JournalFungal Biology Reviews
Volume23
Issue number3
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

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Optical Imaging
Energy Transfer
Recombinant Proteins
Microscopy
Fungi
Cell Biology
Proteins

Keywords

  • Filamentous fungi
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Förster resonance energy transfer (FRET)

Cite this

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title = "In vivo FLIM-FRET measurements of recombinant proteins expressed in filamentous fungi",
abstract = "Fluorescence imaging techniques are extremely powerful tools in cell biology, providing valuable insights into the structure and function of biomolecules in their native environments. In particular, the use of F{\"o}rster resonance energy transfer (FRET) has become increasingly important to obtain information on interactions on the nanoscale, in turn providing insights into molecular behaviour inside living cells. This review describes the basic principles of FRET and fluorescence lifetime imaging microscopy (FLIM) and their application in analyses of protein interactions inside living fungal cells.",
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In vivo FLIM-FRET measurements of recombinant proteins expressed in filamentous fungi. / Altenbach, K.; Duncan, R. R.; Valkonen, Mari (Corresponding Author).

In: Fungal Biology Reviews, Vol. 23, No. 3, 2010, p. 67-71.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - In vivo FLIM-FRET measurements of recombinant proteins expressed in filamentous fungi

AU - Altenbach, K.

AU - Duncan, R. R.

AU - Valkonen, Mari

PY - 2010

Y1 - 2010

N2 - Fluorescence imaging techniques are extremely powerful tools in cell biology, providing valuable insights into the structure and function of biomolecules in their native environments. In particular, the use of Förster resonance energy transfer (FRET) has become increasingly important to obtain information on interactions on the nanoscale, in turn providing insights into molecular behaviour inside living cells. This review describes the basic principles of FRET and fluorescence lifetime imaging microscopy (FLIM) and their application in analyses of protein interactions inside living fungal cells.

AB - Fluorescence imaging techniques are extremely powerful tools in cell biology, providing valuable insights into the structure and function of biomolecules in their native environments. In particular, the use of Förster resonance energy transfer (FRET) has become increasingly important to obtain information on interactions on the nanoscale, in turn providing insights into molecular behaviour inside living cells. This review describes the basic principles of FRET and fluorescence lifetime imaging microscopy (FLIM) and their application in analyses of protein interactions inside living fungal cells.

KW - Filamentous fungi

KW - Fluorescence lifetime imaging microscopy (FLIM)

KW - Förster resonance energy transfer (FRET)

U2 - 10.1016/j.fbr.2009.12.002

DO - 10.1016/j.fbr.2009.12.002

M3 - Article

VL - 23

SP - 67

EP - 71

JO - Fungal Biology Reviews

JF - Fungal Biology Reviews

SN - 1749-4613

IS - 3

ER -