In vivo FLIM-FRET measurements of recombinant proteins expressed in filamentous fungi

K. Altenbach, R. R. Duncan, Mari Valkonen (Corresponding Author)

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fluorescence imaging techniques are extremely powerful tools in cell biology, providing valuable insights into the structure and function of biomolecules in their native environments. In particular, the use of Förster resonance energy transfer (FRET) has become increasingly important to obtain information on interactions on the nanoscale, in turn providing insights into molecular behaviour inside living cells. This review describes the basic principles of FRET and fluorescence lifetime imaging microscopy (FLIM) and their application in analyses of protein interactions inside living fungal cells.
Original languageEnglish
Pages (from-to)67-71
Number of pages5
JournalFungal Biology Reviews
Volume23
Issue number3
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

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Keywords

  • Filamentous fungi
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Förster resonance energy transfer (FRET)

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