Lactobacillus crispatus and its nonaggregating mutant in human colonization trials

S. Cesena, L. Morelli (Corresponding Author), Minna Alander, Tuula Siljander, E. Tuomola, Seppo Salminen, Tiina Mattila-Sandholm, Terttu Vilpponen-Salmela, Atte von Wright

    Research output: Contribution to journalArticleScientificpeer-review

    86 Citations (Scopus)

    Abstract

    A wild-type Lactobacillus crispatus, showing a cell aggregation phenotype and its spontaneous nonaggregating mutant were compared for their in vitro adhesion properties to human ileal mucus and to a cultured human colonic cell line (Caco2) and for their in vivo colonization and adhesion potential with colonoscopy patients as volunteers in feeding trials. The wild-type strain adhered better to mucus or to Caco2 cells than did the mutant. Altogether, three human trials with the wild type and two with the mutant strain were performed. In two of the trials, the wild type could be recovered from either fecal samples or biopsies taken from the colon, while the mutant strain could not be demonstrated in either of the trials where it was used. The L. crispatus colonies recovered from the trials were often mixed, and several enterococci and lactobacillus strains coaggregating with L. crispatus wild type could be isolated. The results indicate that the surface-mediated properties, such as aggregation, of lactobacilli can have a role in adhesion and colonization.
    Original languageEnglish
    Pages (from-to)1001-1010
    Number of pages10
    JournalJournal of Dairy Science
    Volume84
    Issue number5
    DOIs
    Publication statusPublished - 2001
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Lactobacillus crispatus
    Lactobacillus
    Mucus
    adhesion
    mutants
    mucus
    Cell Aggregation
    Surface Properties
    Enterococcus
    colonoscopy
    Colonoscopy
    Volunteers
    Colon
    colon
    volunteers
    Phenotype
    Biopsy
    biopsy
    Cell Line
    cell lines

    Cite this

    Cesena, S., Morelli, L., Alander, M., Siljander, T., Tuomola, E., Salminen, S., ... von Wright, A. (2001). Lactobacillus crispatus and its nonaggregating mutant in human colonization trials. Journal of Dairy Science, 84(5), 1001-1010. https://doi.org/10.3168/jds.S0022-0302(01)74559-6
    Cesena, S. ; Morelli, L. ; Alander, Minna ; Siljander, Tuula ; Tuomola, E. ; Salminen, Seppo ; Mattila-Sandholm, Tiina ; Vilpponen-Salmela, Terttu ; von Wright, Atte. / Lactobacillus crispatus and its nonaggregating mutant in human colonization trials. In: Journal of Dairy Science. 2001 ; Vol. 84, No. 5. pp. 1001-1010.
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    title = "Lactobacillus crispatus and its nonaggregating mutant in human colonization trials",
    abstract = "A wild-type Lactobacillus crispatus, showing a cell aggregation phenotype and its spontaneous nonaggregating mutant were compared for their in vitro adhesion properties to human ileal mucus and to a cultured human colonic cell line (Caco2) and for their in vivo colonization and adhesion potential with colonoscopy patients as volunteers in feeding trials. The wild-type strain adhered better to mucus or to Caco2 cells than did the mutant. Altogether, three human trials with the wild type and two with the mutant strain were performed. In two of the trials, the wild type could be recovered from either fecal samples or biopsies taken from the colon, while the mutant strain could not be demonstrated in either of the trials where it was used. The L. crispatus colonies recovered from the trials were often mixed, and several enterococci and lactobacillus strains coaggregating with L. crispatus wild type could be isolated. The results indicate that the surface-mediated properties, such as aggregation, of lactobacilli can have a role in adhesion and colonization.",
    author = "S. Cesena and L. Morelli and Minna Alander and Tuula Siljander and E. Tuomola and Seppo Salminen and Tiina Mattila-Sandholm and Terttu Vilpponen-Salmela and {von Wright}, Atte",
    year = "2001",
    doi = "10.3168/jds.S0022-0302(01)74559-6",
    language = "English",
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    Cesena, S, Morelli, L, Alander, M, Siljander, T, Tuomola, E, Salminen, S, Mattila-Sandholm, T, Vilpponen-Salmela, T & von Wright, A 2001, 'Lactobacillus crispatus and its nonaggregating mutant in human colonization trials', Journal of Dairy Science, vol. 84, no. 5, pp. 1001-1010. https://doi.org/10.3168/jds.S0022-0302(01)74559-6

    Lactobacillus crispatus and its nonaggregating mutant in human colonization trials. / Cesena, S.; Morelli, L. (Corresponding Author); Alander, Minna; Siljander, Tuula; Tuomola, E.; Salminen, Seppo; Mattila-Sandholm, Tiina; Vilpponen-Salmela, Terttu; von Wright, Atte.

    In: Journal of Dairy Science, Vol. 84, No. 5, 2001, p. 1001-1010.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Lactobacillus crispatus and its nonaggregating mutant in human colonization trials

    AU - Cesena, S.

    AU - Morelli, L.

    AU - Alander, Minna

    AU - Siljander, Tuula

    AU - Tuomola, E.

    AU - Salminen, Seppo

    AU - Mattila-Sandholm, Tiina

    AU - Vilpponen-Salmela, Terttu

    AU - von Wright, Atte

    PY - 2001

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    AB - A wild-type Lactobacillus crispatus, showing a cell aggregation phenotype and its spontaneous nonaggregating mutant were compared for their in vitro adhesion properties to human ileal mucus and to a cultured human colonic cell line (Caco2) and for their in vivo colonization and adhesion potential with colonoscopy patients as volunteers in feeding trials. The wild-type strain adhered better to mucus or to Caco2 cells than did the mutant. Altogether, three human trials with the wild type and two with the mutant strain were performed. In two of the trials, the wild type could be recovered from either fecal samples or biopsies taken from the colon, while the mutant strain could not be demonstrated in either of the trials where it was used. The L. crispatus colonies recovered from the trials were often mixed, and several enterococci and lactobacillus strains coaggregating with L. crispatus wild type could be isolated. The results indicate that the surface-mediated properties, such as aggregation, of lactobacilli can have a role in adhesion and colonization.

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