Imaging the last stages of protein secretion in the filamentous fungus Trichoderma reesei

Mari Valkonen (Corresponding author), Merja Penttilä, Markku Saloheimo, Nick Read

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

    Abstract

    Available data suggest that the basic principles of secretion obtained from studies on S. cerevisiae and animal cells also apply to filamentous fungi. Although considerable research has been performed on the secretion process in filamentous fungi, little is known about their secretory machinery. The transport of proteins between the cellular compartments occurs in membrane-bounded vesicles that bud from the donor and fuse with the acceptor compartment. Membrane bound proteins called SNAREs (Soluble N-ethylaleimide-sensitive factor Attachment protein REceptors) play a key role in membrane fusion. SNAREs have been found to be involved in most membrane fusion events in the cell. This study describes the cloning and characterization of a putative exocytotic t-SNARE (sso1) from Trichoderma reesei. We cloned the T. reesei homologue of Neurospora crassa nsyn2. This protein was labelled with the Cerulean fluorescent protein mCer and the fusion protein expressed in T. reesei. Transformants expressing the fusion protein exhibit a clear labelling of the plasma membrane. The fusion protein is uniformly distributed along the hyphae with no tip-focused gradient. A v-SNARE SNCI was labelled with mRFP to analyze its interaction with the SSOI protein using fluorescence resonance energy
    transfer (FRET) imaging.
    Original languageEnglish
    Title of host publication8th European Conference on Fungal Genetics
    Subtitle of host publicationBook of Abstracts
    EditorsIrina S. Druzhinina, Alexey G. Kopchinskiy, Christian P. Kubicek
    PublisherVienna University of Technology
    ChapterVIII Protein Targeting and Secretion
    Pages299
    Publication statusPublished - 2006
    Event8th European Conference on Fungal Genetics - Vienna, Austria
    Duration: 8 Apr 200611 Apr 2006

    Conference

    Conference8th European Conference on Fungal Genetics
    Country/TerritoryAustria
    CityVienna
    Period8/04/0611/04/06

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