Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide

Rene Verhoef; Pieter de Waard; Henk A. Schols; Matti Siika-aho; Alphons G.J. Voragen

doi:10.1016/S0008-6215(03)00261-1

Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide

Rene Verhoef, Pieter de Waard, Henk A. Schols, Matti Siika-aho, Alphons G.J. Voragen (Corresponding Author)

Wageningen University & Research (WUR)

Research output: Contribution to journal › Article › Scientific › peer-review

46 Citations (Scopus)

Abstract

The slime-forming bacterium Methylobacterium sp. was isolated from a Finnish paper machine and its exopolysaccharide (EPS) was produced on laboratory scale. Sugar compositional analysis revealed a 100% galactan (EPS). However, FT-IR showed a very strong peak at 1611 cm⁻¹ showing the presence of pyruvate. Analysis of the pyruvate content revealed that, based on the sugar composition, the EPS consists of a trisaccharide repeating unit consisting of d-galactopyranose and [4,6-O-(1-carboxyethylidene)]-d-galactopyranose with a molar ratio of 1:2, respectively. Both linkage analysis and 2D homo- and heteronuclear ¹H and ¹³C NMR spectroscopy revealed the following repeating unit: →3)-[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)-α-d-Galp-(1→. By enrichment cultures from various ground and compost heap samples a polysaccharide-degrading culture was obtained that produced an endo acting enzyme able to degrade the EPS described. The enzyme hydrolysed the EPS to a large extent, releasing oligomers that mainly consisted out of two repeating units.

Original language	English
Pages (from-to)	1851-1859
Number of pages	9
Journal	Carbohydrate Research
Volume	338
Issue number	18
DOIs	https://doi.org/10.1016/S0008-6215(03)00261-1
Publication status	Published - 2003
MoE publication type	A1 Journal article-refereed

Keywords

Methylobacterium sp.
EPS
Pulp and paper industry
Pink slime
Enzymes
Galactan
Pyruvate
NMR

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title = "Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide",

abstract = "The slime-forming bacterium Methylobacterium sp. was isolated from a Finnish paper machine and its exopolysaccharide (EPS) was produced on laboratory scale. Sugar compositional analysis revealed a 100% galactan (EPS). However, FT-IR showed a very strong peak at 1611 cm−1 showing the presence of pyruvate. Analysis of the pyruvate content revealed that, based on the sugar composition, the EPS consists of a trisaccharide repeating unit consisting of d-galactopyranose and [4,6-O-(1-carboxyethylidene)]-d-galactopyranose with a molar ratio of 1:2, respectively. Both linkage analysis and 2D homo- and heteronuclear 1H and 13C NMR spectroscopy revealed the following repeating unit: →3)-[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)-α-d-Galp-(1→. By enrichment cultures from various ground and compost heap samples a polysaccharide-degrading culture was obtained that produced an endo acting enzyme able to degrade the EPS described. The enzyme hydrolysed the EPS to a large extent, releasing oligomers that mainly consisted out of two repeating units.",

keywords = "Methylobacterium sp., EPS, Pulp and paper industry, Pink slime, Enzymes, Galactan, Pyruvate, NMR",

author = "Rene Verhoef and {de Waard}, Pieter and Schols, {Henk A.} and Matti Siika-aho and Voragen, {Alphons G.J.}",

year = "2003",

doi = "10.1016/S0008-6215(03)00261-1",

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T1 - Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide

AU - Verhoef, Rene

AU - de Waard, Pieter

AU - Schols, Henk A.

AU - Siika-aho, Matti

AU - Voragen, Alphons G.J.

PY - 2003

Y1 - 2003

N2 - The slime-forming bacterium Methylobacterium sp. was isolated from a Finnish paper machine and its exopolysaccharide (EPS) was produced on laboratory scale. Sugar compositional analysis revealed a 100% galactan (EPS). However, FT-IR showed a very strong peak at 1611 cm−1 showing the presence of pyruvate. Analysis of the pyruvate content revealed that, based on the sugar composition, the EPS consists of a trisaccharide repeating unit consisting of d-galactopyranose and [4,6-O-(1-carboxyethylidene)]-d-galactopyranose with a molar ratio of 1:2, respectively. Both linkage analysis and 2D homo- and heteronuclear 1H and 13C NMR spectroscopy revealed the following repeating unit: →3)-[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)-α-d-Galp-(1→. By enrichment cultures from various ground and compost heap samples a polysaccharide-degrading culture was obtained that produced an endo acting enzyme able to degrade the EPS described. The enzyme hydrolysed the EPS to a large extent, releasing oligomers that mainly consisted out of two repeating units.

AB - The slime-forming bacterium Methylobacterium sp. was isolated from a Finnish paper machine and its exopolysaccharide (EPS) was produced on laboratory scale. Sugar compositional analysis revealed a 100% galactan (EPS). However, FT-IR showed a very strong peak at 1611 cm−1 showing the presence of pyruvate. Analysis of the pyruvate content revealed that, based on the sugar composition, the EPS consists of a trisaccharide repeating unit consisting of d-galactopyranose and [4,6-O-(1-carboxyethylidene)]-d-galactopyranose with a molar ratio of 1:2, respectively. Both linkage analysis and 2D homo- and heteronuclear 1H and 13C NMR spectroscopy revealed the following repeating unit: →3)-[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)-α-d-Galp-(1→. By enrichment cultures from various ground and compost heap samples a polysaccharide-degrading culture was obtained that produced an endo acting enzyme able to degrade the EPS described. The enzyme hydrolysed the EPS to a large extent, releasing oligomers that mainly consisted out of two repeating units.

KW - Methylobacterium sp.

KW - EPS

KW - Pulp and paper industry

KW - Pink slime

KW - Enzymes

KW - Galactan

KW - Pyruvate

KW - NMR

U2 - 10.1016/S0008-6215(03)00261-1

DO - 10.1016/S0008-6215(03)00261-1

M3 - Article

SN - 0008-6215

VL - 338

SP - 1851

EP - 1859

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 18

ER -

Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide

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Keywords

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