Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide

Rene Verhoef, Pieter de Waard, Henk A. Schols, Matti Siika-aho, Alphons G.J. Voragen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)

Abstract

The slime-forming bacterium Methylobacterium sp. was isolated from a Finnish paper machine and its exopolysaccharide (EPS) was produced on laboratory scale. Sugar compositional analysis revealed a 100% galactan (EPS). However, FT-IR showed a very strong peak at 1611 cm−1 showing the presence of pyruvate. Analysis of the pyruvate content revealed that, based on the sugar composition, the EPS consists of a trisaccharide repeating unit consisting of d-galactopyranose and [4,6-O-(1-carboxyethylidene)]-d-galactopyranose with a molar ratio of 1:2, respectively. Both linkage analysis and 2D homo- and heteronuclear 1H and 13C NMR spectroscopy revealed the following repeating unit: →3)-[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)-α-d-Galp-(1→. By enrichment cultures from various ground and compost heap samples a polysaccharide-degrading culture was obtained that produced an endo acting enzyme able to degrade the EPS described. The enzyme hydrolysed the EPS to a large extent, releasing oligomers that mainly consisted out of two repeating units.
Original languageEnglish
Pages (from-to)1851-1859
Number of pages9
JournalCarbohydrate Research
Volume338
Issue number18
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

Fingerprint

Methylobacterium
Galactans
Pyruvic Acid
Galactose
Sugars
Myxococcales
Biomolecular Nuclear Magnetic Resonance
Trisaccharides
Enzymes
Oligomers
Nuclear magnetic resonance spectroscopy
Polysaccharides
Bacteria
Magnetic Resonance Spectroscopy
Soil
Chemical analysis

Keywords

  • Methylobacterium sp.
  • EPS
  • Pulp and paper industry
  • Pink slime
  • Enzymes
  • Galactan
  • Pyruvate
  • NMR

Cite this

Verhoef, Rene ; de Waard, Pieter ; Schols, Henk A. ; Siika-aho, Matti ; Voragen, Alphons G.J. / Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide. In: Carbohydrate Research. 2003 ; Vol. 338, No. 18. pp. 1851-1859.
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abstract = "The slime-forming bacterium Methylobacterium sp. was isolated from a Finnish paper machine and its exopolysaccharide (EPS) was produced on laboratory scale. Sugar compositional analysis revealed a 100{\%} galactan (EPS). However, FT-IR showed a very strong peak at 1611 cm−1 showing the presence of pyruvate. Analysis of the pyruvate content revealed that, based on the sugar composition, the EPS consists of a trisaccharide repeating unit consisting of d-galactopyranose and [4,6-O-(1-carboxyethylidene)]-d-galactopyranose with a molar ratio of 1:2, respectively. Both linkage analysis and 2D homo- and heteronuclear 1H and 13C NMR spectroscopy revealed the following repeating unit: →3)-[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)-α-d-Galp-(1→. By enrichment cultures from various ground and compost heap samples a polysaccharide-degrading culture was obtained that produced an endo acting enzyme able to degrade the EPS described. The enzyme hydrolysed the EPS to a large extent, releasing oligomers that mainly consisted out of two repeating units.",
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author = "Rene Verhoef and {de Waard}, Pieter and Schols, {Henk A.} and Matti Siika-aho and Voragen, {Alphons G.J.}",
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Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide. / Verhoef, Rene; de Waard, Pieter; Schols, Henk A.; Siika-aho, Matti; Voragen, Alphons G.J. (Corresponding Author).

In: Carbohydrate Research, Vol. 338, No. 18, 2003, p. 1851-1859.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide

AU - Verhoef, Rene

AU - de Waard, Pieter

AU - Schols, Henk A.

AU - Siika-aho, Matti

AU - Voragen, Alphons G.J.

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N2 - The slime-forming bacterium Methylobacterium sp. was isolated from a Finnish paper machine and its exopolysaccharide (EPS) was produced on laboratory scale. Sugar compositional analysis revealed a 100% galactan (EPS). However, FT-IR showed a very strong peak at 1611 cm−1 showing the presence of pyruvate. Analysis of the pyruvate content revealed that, based on the sugar composition, the EPS consists of a trisaccharide repeating unit consisting of d-galactopyranose and [4,6-O-(1-carboxyethylidene)]-d-galactopyranose with a molar ratio of 1:2, respectively. Both linkage analysis and 2D homo- and heteronuclear 1H and 13C NMR spectroscopy revealed the following repeating unit: →3)-[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)-α-d-Galp-(1→. By enrichment cultures from various ground and compost heap samples a polysaccharide-degrading culture was obtained that produced an endo acting enzyme able to degrade the EPS described. The enzyme hydrolysed the EPS to a large extent, releasing oligomers that mainly consisted out of two repeating units.

AB - The slime-forming bacterium Methylobacterium sp. was isolated from a Finnish paper machine and its exopolysaccharide (EPS) was produced on laboratory scale. Sugar compositional analysis revealed a 100% galactan (EPS). However, FT-IR showed a very strong peak at 1611 cm−1 showing the presence of pyruvate. Analysis of the pyruvate content revealed that, based on the sugar composition, the EPS consists of a trisaccharide repeating unit consisting of d-galactopyranose and [4,6-O-(1-carboxyethylidene)]-d-galactopyranose with a molar ratio of 1:2, respectively. Both linkage analysis and 2D homo- and heteronuclear 1H and 13C NMR spectroscopy revealed the following repeating unit: →3)-[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)[4,6-O-(1-carboxyethylidene)]-α-d-Galp-(1→3)-α-d-Galp-(1→. By enrichment cultures from various ground and compost heap samples a polysaccharide-degrading culture was obtained that produced an endo acting enzyme able to degrade the EPS described. The enzyme hydrolysed the EPS to a large extent, releasing oligomers that mainly consisted out of two repeating units.

KW - Methylobacterium sp.

KW - EPS

KW - Pulp and paper industry

KW - Pink slime

KW - Enzymes

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KW - Pyruvate

KW - NMR

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