TY - JOUR
T1 - Immobilization of a phosphonium ionic liquid on a silica monolith for hydrophilic interaction chromatography
AU - Moravcová, Dana
AU - Planeta, Josef
AU - King, Alistair W.T.
AU - Wiedmer, Susanne K.
N1 - Funding Information:
This contribution has been supported by the Czech Science Foundation (Project No. 16-03749S), by the Ministry of the Interior of the Czech Republic (Project No.VI20172020069), and by the Czech Academy of Sciences (institutional support RVO:68081715 ). The Academy of Finland (project number 266342; SKW) is acknowledged for funding.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic capillary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm were modified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via 3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic interaction liquid chromatography separation conditions, employing a sample mixture containing purine and pyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silica monolith was preserved even after modification, and it reached values in the range of 98,000–174,000 theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence of additives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phase on the separation of some selected analytes were investigated. The prepared columns showed different separation selectivity compared to silica, phenyl and sulfobetaine stationary phases.
AB - A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic capillary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm were modified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via 3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic interaction liquid chromatography separation conditions, employing a sample mixture containing purine and pyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silica monolith was preserved even after modification, and it reached values in the range of 98,000–174,000 theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence of additives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phase on the separation of some selected analytes were investigated. The prepared columns showed different separation selectivity compared to silica, phenyl and sulfobetaine stationary phases.
KW - HILIC
KW - Nucleobase
KW - Nucleoside
KW - Silica monolithic capillary column
KW - Chromatography, Liquid/methods
KW - Silicon Dioxide/chemistry
KW - Reproducibility of Results
KW - Ionic Liquids/chemical synthesis
KW - Organophosphorus Compounds/chemistry
KW - Nucleosides/analysis
KW - Betaine/analogs & derivatives
KW - Hydrophobic and Hydrophilic Interactions
KW - Porosity
UR - http://www.scopus.com/inward/record.url?scp=85045085429&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2018.04.012
DO - 10.1016/j.chroma.2018.04.012
M3 - Article
C2 - 29653778
AN - SCOPUS:85045085429
SN - 0021-9673
VL - 1552
SP - 53
EP - 59
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -