Immobilization of a phosphonium ionic liquid on a silica monolith for hydrophilic interaction chromatography

Dana Moravcová, Josef Planeta, Alistair W.T. King, Susanne K. Wiedmer

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic capillary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm were modified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via 3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic interaction liquid chromatography separation conditions, employing a sample mixture containing purine and pyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silica monolith was preserved even after modification, and it reached values in the range of 98,000–174,000 theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence of additives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phase on the separation of some selected analytes were investigated. The prepared columns showed different separation selectivity compared to silica, phenyl and sulfobetaine stationary phases.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalJournal of Chromatography A
Volume1552
DOIs
Publication statusPublished - 1 Jun 2018
MoE publication typeA1 Journal article-refereed

Keywords

  • HILIC
  • Nucleobase
  • Nucleoside
  • Silica monolithic capillary column
  • Chromatography, Liquid/methods
  • Silicon Dioxide/chemistry
  • Reproducibility of Results
  • Ionic Liquids/chemical synthesis
  • Organophosphorus Compounds/chemistry
  • Nucleosides/analysis
  • Betaine/analogs & derivatives
  • Hydrophobic and Hydrophilic Interactions
  • Porosity

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