Immobilization of a phosphonium ionic liquid on a silica monolith for hydrophilic interaction chromatography

Dana Moravcová; Josef Planeta; Alistair W.T. King; Susanne K. Wiedmer

doi:10.1016/j.chroma.2018.04.012

Immobilization of a phosphonium ionic liquid on a silica monolith for hydrophilic interaction chromatography

Dana Moravcová^*, Josef Planeta, Alistair W.T. King, Susanne K. Wiedmer

^*Corresponding author for this work

Not published at VTT

Research output: Contribution to journal › Article › Scientific › peer-review

21 Citations (Scopus)

Abstract

A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic capillary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm were modified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via 3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic interaction liquid chromatography separation conditions, employing a sample mixture containing purine and pyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silica monolith was preserved even after modification, and it reached values in the range of 98,000–174,000 theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence of additives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phase on the separation of some selected analytes were investigated. The prepared columns showed different separation selectivity compared to silica, phenyl and sulfobetaine stationary phases.

Original language	English
Pages (from-to)	53-59
Number of pages	7
Journal	Journal of Chromatography A
Volume	1552
DOIs	https://doi.org/10.1016/j.chroma.2018.04.012
Publication status	Published - 1 Jun 2018
MoE publication type	A1 Journal article-refereed

Funding

This contribution has been supported by the Czech Science Foundation (Project No. 16-03749S), by the Ministry of the Interior of the Czech Republic (Project No.VI20172020069), and by the Czech Academy of Sciences (institutional support RVO:68081715 ). The Academy of Finland (project number 266342; SKW) is acknowledged for funding.

Keywords

HILIC
Nucleobase
Nucleoside
Silica monolithic capillary column
Chromatography, Liquid/methods
Silicon Dioxide/chemistry
Reproducibility of Results
Ionic Liquids/chemical synthesis
Organophosphorus Compounds/chemistry
Nucleosides/analysis
Betaine/analogs & derivatives
Hydrophobic and Hydrophilic Interactions
Porosity

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10.1016/j.chroma.2018.04.012

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title = "Immobilization of a phosphonium ionic liquid on a silica monolith for hydrophilic interaction chromatography",

abstract = "A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic capillary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm were modified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via 3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic interaction liquid chromatography separation conditions, employing a sample mixture containing purine and pyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silica monolith was preserved even after modification, and it reached values in the range of 98,000–174,000 theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence of additives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phase on the separation of some selected analytes were investigated. The prepared columns showed different separation selectivity compared to silica, phenyl and sulfobetaine stationary phases.",

keywords = "HILIC, Nucleobase, Nucleoside, Silica monolithic capillary column, Chromatography, Liquid/methods, Silicon Dioxide/chemistry, Reproducibility of Results, Ionic Liquids/chemical synthesis, Organophosphorus Compounds/chemistry, Nucleosides/analysis, Betaine/analogs \& derivatives, Hydrophobic and Hydrophilic Interactions, Porosity",

author = "Dana Moravcov{\'a} and Josef Planeta and King, \{Alistair W.T.\} and Wiedmer, \{Susanne K.\}",

note = "Funding Information: This contribution has been supported by the Czech Science Foundation (Project No. 16-03749S), by the Ministry of the Interior of the Czech Republic (Project No.VI20172020069), and by the Czech Academy of Sciences (institutional support RVO:68081715 ). The Academy of Finland (project number 266342; SKW) is acknowledged for funding. Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2018",

month = jun,

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doi = "10.1016/j.chroma.2018.04.012",

language = "English",

volume = "1552",

pages = "53--59",

journal = "Journal of Chromatography A",

issn = "0021-9673",

publisher = "Elsevier",

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TY - JOUR

T1 - Immobilization of a phosphonium ionic liquid on a silica monolith for hydrophilic interaction chromatography

AU - Moravcová, Dana

AU - Planeta, Josef

AU - King, Alistair W.T.

AU - Wiedmer, Susanne K.

N1 - Funding Information: This contribution has been supported by the Czech Science Foundation (Project No. 16-03749S), by the Ministry of the Interior of the Czech Republic (Project No.VI20172020069), and by the Czech Academy of Sciences (institutional support RVO:68081715 ). The Academy of Finland (project number 266342; SKW) is acknowledged for funding. Publisher Copyright: © 2018 Elsevier B.V.

PY - 2018/6/1

Y1 - 2018/6/1

N2 - A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic capillary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm were modified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via 3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic interaction liquid chromatography separation conditions, employing a sample mixture containing purine and pyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silica monolith was preserved even after modification, and it reached values in the range of 98,000–174,000 theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence of additives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phase on the separation of some selected analytes were investigated. The prepared columns showed different separation selectivity compared to silica, phenyl and sulfobetaine stationary phases.

AB - A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic capillary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm were modified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via 3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic interaction liquid chromatography separation conditions, employing a sample mixture containing purine and pyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silica monolith was preserved even after modification, and it reached values in the range of 98,000–174,000 theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence of additives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phase on the separation of some selected analytes were investigated. The prepared columns showed different separation selectivity compared to silica, phenyl and sulfobetaine stationary phases.

KW - HILIC

KW - Nucleobase

KW - Nucleoside

KW - Silica monolithic capillary column

KW - Chromatography, Liquid/methods

KW - Silicon Dioxide/chemistry

KW - Reproducibility of Results

KW - Ionic Liquids/chemical synthesis

KW - Organophosphorus Compounds/chemistry

KW - Nucleosides/analysis

KW - Betaine/analogs & derivatives

KW - Hydrophobic and Hydrophilic Interactions

KW - Porosity

UR - https://www.scopus.com/pages/publications/85045085429

U2 - 10.1016/j.chroma.2018.04.012

DO - 10.1016/j.chroma.2018.04.012

M3 - Article

C2 - 29653778

AN - SCOPUS:85045085429

SN - 0021-9673

VL - 1552

SP - 53

EP - 59

JO - Journal of Chromatography A

JF - Journal of Chromatography A

ER -

Immobilization of a phosphonium ionic liquid on a silica monolith for hydrophilic interaction chromatography

Abstract

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Keywords

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