TY - JOUR
T1 - Immobilization of natural lipid biomembranes and their interactions with choline carboxylates. A nanoplasmonic sensing study
AU - Duša, Filip
AU - Chen, Wen
AU - Witos, Joanna
AU - Rantamäki, Antti H.
AU - King, Alistair W.T.
AU - Sklavounos, Evangelos
AU - Roth, Michal
AU - Wiedmer, Susanne K.
N1 - Funding Information:
This work was supported by the Programme for research and mobility support of starting researchers of the Czech Academy of Sciences number MSM200311602 (F.D.) and by institutional support RVO:68081715 (F.D.). Academy of Finland (grant number 266342 ) is acknowledged for the financial support (S.K·W). Financial support from Kemira Oyj is acknowledged (E.S. & A. W. T. K.).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - The cell membrane is mainly composed of lipid bilayers with inserted proteins and carbohydrates. Lipid bilayers made of purified or synthetic lipids are widely used for estimating the effect of target compounds on cell membranes. However, the composition of such biomimetic membranes is much simpler than the composition of biological membranes. Interactions between compounds and simple composition biomimetic membranes might not demonstrate the effect of target compounds as precisely as membranes with compositions close to real organisms. Therefore, the aim of our study is to construct biomimetic membrane closely mimicking the state of natural membranes. Liposomes were prepared from lipids extracted from L-α-phosphatidylcholine, Escherichia coli, yeast (Saccharomyces cerevisiae) and bovine liver cells through agitation and sonication. They were immobilized onto silicon dioxide (SiO2) sensor surfaces using N-(2-hydroxyethyl)piperazine-N′-2-ethanesulfonic acid buffer with calcium chloride. The biomimetic membranes were successfully immobilized onto the SiO2 sensor surface and detected by nanoplasmonic sensing. The immobilized membranes were exposed to choline carboxylates. The membrane disruption effect was, as expected, more pronounced with increasing carbohydrate chain length of the carboxylates. The results correlated with the toxicity values determined using Vibrio fischeri bacteria. The yeast extracted lipid membranes had the strongest response to introduction of choline laurate while the bovine liver lipid extracted liposomes were the most sensitive towards the shorter choline carboxylates. This implies that the composition of the cell membrane plays a crucial role upon interaction with choline carboxylates, and underlines the necessity of testing membrane systems of different origin to obtain an overall image of such interactions.
AB - The cell membrane is mainly composed of lipid bilayers with inserted proteins and carbohydrates. Lipid bilayers made of purified or synthetic lipids are widely used for estimating the effect of target compounds on cell membranes. However, the composition of such biomimetic membranes is much simpler than the composition of biological membranes. Interactions between compounds and simple composition biomimetic membranes might not demonstrate the effect of target compounds as precisely as membranes with compositions close to real organisms. Therefore, the aim of our study is to construct biomimetic membrane closely mimicking the state of natural membranes. Liposomes were prepared from lipids extracted from L-α-phosphatidylcholine, Escherichia coli, yeast (Saccharomyces cerevisiae) and bovine liver cells through agitation and sonication. They were immobilized onto silicon dioxide (SiO2) sensor surfaces using N-(2-hydroxyethyl)piperazine-N′-2-ethanesulfonic acid buffer with calcium chloride. The biomimetic membranes were successfully immobilized onto the SiO2 sensor surface and detected by nanoplasmonic sensing. The immobilized membranes were exposed to choline carboxylates. The membrane disruption effect was, as expected, more pronounced with increasing carbohydrate chain length of the carboxylates. The results correlated with the toxicity values determined using Vibrio fischeri bacteria. The yeast extracted lipid membranes had the strongest response to introduction of choline laurate while the bovine liver lipid extracted liposomes were the most sensitive towards the shorter choline carboxylates. This implies that the composition of the cell membrane plays a crucial role upon interaction with choline carboxylates, and underlines the necessity of testing membrane systems of different origin to obtain an overall image of such interactions.
KW - Choline carboxylate
KW - Immobilization
KW - Interaction
KW - Ionic liquid
KW - Nanoplasmonic sensing
KW - Natural lipid biomembrane
KW - Choline/analogs & derivatives
KW - Liposomes/chemistry
KW - Membrane Lipids/chemistry
KW - Biomimetic Materials/chemistry
KW - Animals
KW - Cattle
KW - Cell Membrane/chemistry
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=85075520531&partnerID=8YFLogxK
U2 - 10.1016/j.bbamem.2019.183115
DO - 10.1016/j.bbamem.2019.183115
M3 - Article
C2 - 31704086
AN - SCOPUS:85075520531
SN - 0005-2736
VL - 1862
JO - Biochimica et Biophysica Acta: Biomembranes
JF - Biochimica et Biophysica Acta: Biomembranes
IS - 2
M1 - 183115
ER -