Immobilization-stabilization of proteins on nanofibrillated cellulose derivatives and their bioactive film formation

Suvi Arola (Corresponding Author), Tekla Tammelin, Harri Setälä, Antti Tullila, Markus B. Linder

Research output: Contribution to journalArticleScientificpeer-review

59 Citations (Scopus)

Abstract

In a number of different applications for enzymes and specific binding proteins a key technology is the immobilization of these proteins to different types of supports. In this work we describe a concept for protein immobilization that is based on nanofibrillated cellulose (NFC). NFC is a form of cellulose where fibers have been disintegrated into fibrils that are only a few nanometers in diameter and have a very large aspect ratio. Proteins were conjugated through three different strategies using amine, epoxy, and carboxylic acid functionalized NFC. The conjugation chemistries were chosen according to the reactive groups on the NFC derivatives; epoxy amination, heterobifunctional modification of amino groups, and EDC/s-NHS activation of carboxylic acid groups. The conjugation reactions were performed in solution and immobilization was performed by spin coating the protein-NCF conjugates. The structure of NFC was shown to be advantageous for both protein performance and stability. The use of NFC allows all covalent chemistry to be performed in solution, while the immobilization is achieved by a simple spin coating or spreading of the protein-NFC conjugates on a support. This allows more scalable methods and better control of conditions compared to the traditional methods that depend on surface reactions.

Original languageEnglish
Pages (from-to)594-603
Number of pages10
JournalBiomacromolecules
Volume13
Issue number3
DOIs
Publication statusPublished - 12 Mar 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulose derivatives
Cellulose
Stabilization
Proteins
Spin coating
Carboxylic Acids
Carboxylic acids
Amination
Surface reactions
Amines
Aspect ratio
Carrier Proteins
Enzymes
Chemical activation
Fibers

Keywords

  • Alkaline Phosphatase
  • Amino group
  • Bioactive films
  • Carboxylic acid groups
  • Conjugation chemistry
  • Covalent chemistry
  • Nanofibrillated cellulose
  • Protein immobilization
  • Polysaccharides

Cite this

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title = "Immobilization-stabilization of proteins on nanofibrillated cellulose derivatives and their bioactive film formation",
abstract = "In a number of different applications for enzymes and specific binding proteins a key technology is the immobilization of these proteins to different types of supports. In this work we describe a concept for protein immobilization that is based on nanofibrillated cellulose (NFC). NFC is a form of cellulose where fibers have been disintegrated into fibrils that are only a few nanometers in diameter and have a very large aspect ratio. Proteins were conjugated through three different strategies using amine, epoxy, and carboxylic acid functionalized NFC. The conjugation chemistries were chosen according to the reactive groups on the NFC derivatives; epoxy amination, heterobifunctional modification of amino groups, and EDC/s-NHS activation of carboxylic acid groups. The conjugation reactions were performed in solution and immobilization was performed by spin coating the protein-NCF conjugates. The structure of NFC was shown to be advantageous for both protein performance and stability. The use of NFC allows all covalent chemistry to be performed in solution, while the immobilization is achieved by a simple spin coating or spreading of the protein-NFC conjugates on a support. This allows more scalable methods and better control of conditions compared to the traditional methods that depend on surface reactions.",
keywords = "Alkaline Phosphatase, Amino group, Bioactive films, Carboxylic acid groups, Conjugation chemistry, Covalent chemistry, Nanofibrillated cellulose, Protein immobilization, Polysaccharides",
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Immobilization-stabilization of proteins on nanofibrillated cellulose derivatives and their bioactive film formation. / Arola, Suvi (Corresponding Author); Tammelin, Tekla; Setälä, Harri; Tullila, Antti; Linder, Markus B.

In: Biomacromolecules, Vol. 13, No. 3, 12.03.2012, p. 594-603.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Arola, Suvi

AU - Tammelin, Tekla

AU - Setälä, Harri

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AU - Linder, Markus B.

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N2 - In a number of different applications for enzymes and specific binding proteins a key technology is the immobilization of these proteins to different types of supports. In this work we describe a concept for protein immobilization that is based on nanofibrillated cellulose (NFC). NFC is a form of cellulose where fibers have been disintegrated into fibrils that are only a few nanometers in diameter and have a very large aspect ratio. Proteins were conjugated through three different strategies using amine, epoxy, and carboxylic acid functionalized NFC. The conjugation chemistries were chosen according to the reactive groups on the NFC derivatives; epoxy amination, heterobifunctional modification of amino groups, and EDC/s-NHS activation of carboxylic acid groups. The conjugation reactions were performed in solution and immobilization was performed by spin coating the protein-NCF conjugates. The structure of NFC was shown to be advantageous for both protein performance and stability. The use of NFC allows all covalent chemistry to be performed in solution, while the immobilization is achieved by a simple spin coating or spreading of the protein-NFC conjugates on a support. This allows more scalable methods and better control of conditions compared to the traditional methods that depend on surface reactions.

AB - In a number of different applications for enzymes and specific binding proteins a key technology is the immobilization of these proteins to different types of supports. In this work we describe a concept for protein immobilization that is based on nanofibrillated cellulose (NFC). NFC is a form of cellulose where fibers have been disintegrated into fibrils that are only a few nanometers in diameter and have a very large aspect ratio. Proteins were conjugated through three different strategies using amine, epoxy, and carboxylic acid functionalized NFC. The conjugation chemistries were chosen according to the reactive groups on the NFC derivatives; epoxy amination, heterobifunctional modification of amino groups, and EDC/s-NHS activation of carboxylic acid groups. The conjugation reactions were performed in solution and immobilization was performed by spin coating the protein-NCF conjugates. The structure of NFC was shown to be advantageous for both protein performance and stability. The use of NFC allows all covalent chemistry to be performed in solution, while the immobilization is achieved by a simple spin coating or spreading of the protein-NFC conjugates on a support. This allows more scalable methods and better control of conditions compared to the traditional methods that depend on surface reactions.

KW - Alkaline Phosphatase

KW - Amino group

KW - Bioactive films

KW - Carboxylic acid groups

KW - Conjugation chemistry

KW - Covalent chemistry

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KW - Protein immobilization

KW - Polysaccharides

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