Impact of a very low-energy diet on the fecal microbiota of obese individuals

C D Simões (Corresponding Author), Johanna Maukonen, K P Scott, K A Virtanen, K H Pietikäinen, Maria Saarela

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Study how the dietary intake affects the fecal microbiota of a group of obese individuals after a 6-week very low-energy diet (VLED) and thereafter during a follow-up period of 5, 8, and 12 months. Additionally, we compared two different methods, fluorescent in situ hybridization (FISH) and real-time PCR (qPCR), for the quantification of fecal samples. Sixteen subjects participated in a 12-month dietary intervention which consisted of a VLED high in protein and low in carbohydrates followed by a personalized diet plan, combined with exercise and lifestyle counseling. Fecal samples were analyzed using qPCR, FISH, and denaturing gradient gel electrophoresis. The VLED affected the fecal microbiota, in particular bifidobacteria that decreased approximately two logs compared with the baseline numbers. The change in numbers of the bacterial groups studied followed the dietary intake and not the weight variations during the 12-month intervention. Methanogens were detected in 56 % of the participants at every sampling point, regardless of the dietary intake. Moreover, although absolute numbers of comparable bacterial groups were similar between FISH and qPCR measurements, relative proportions were higher according to FISH results. Changes in the fecal microbial numbers of obese individuals were primarily affected by the dietary intake rather than weight changes.
Original languageEnglish
Pages (from-to)1421-1429
Number of pages9
JournalEuropean Journal of Nutrition
Volume53
Issue number6
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

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Microbiota
Fluorescence In Situ Hybridization
Diet
Weights and Measures
Denaturing Gradient Gel Electrophoresis
Bifidobacterium
Life Style
Counseling
Real-Time Polymerase Chain Reaction
Carbohydrates
Proteins

Cite this

Simões, C. D., Maukonen, J., Scott, K. P., Virtanen, K. A., Pietikäinen, K. H., & Saarela, M. (2014). Impact of a very low-energy diet on the fecal microbiota of obese individuals. European Journal of Nutrition, 53(6), 1421-1429. https://doi.org/10.1007/s00394-013-0645-0
Simões, C D ; Maukonen, Johanna ; Scott, K P ; Virtanen, K A ; Pietikäinen, K H ; Saarela, Maria. / Impact of a very low-energy diet on the fecal microbiota of obese individuals. In: European Journal of Nutrition. 2014 ; Vol. 53, No. 6. pp. 1421-1429.
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Simões, CD, Maukonen, J, Scott, KP, Virtanen, KA, Pietikäinen, KH & Saarela, M 2014, 'Impact of a very low-energy diet on the fecal microbiota of obese individuals', European Journal of Nutrition, vol. 53, no. 6, pp. 1421-1429. https://doi.org/10.1007/s00394-013-0645-0

Impact of a very low-energy diet on the fecal microbiota of obese individuals. / Simões, C D (Corresponding Author); Maukonen, Johanna; Scott, K P; Virtanen, K A; Pietikäinen, K H; Saarela, Maria.

In: European Journal of Nutrition, Vol. 53, No. 6, 2014, p. 1421-1429.

Research output: Contribution to journalArticleScientificpeer-review

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Simões CD, Maukonen J, Scott KP, Virtanen KA, Pietikäinen KH, Saarela M. Impact of a very low-energy diet on the fecal microbiota of obese individuals. European Journal of Nutrition. 2014;53(6):1421-1429. https://doi.org/10.1007/s00394-013-0645-0