Impact of hydrothermal pre-treatment to chemical composition, enzymatic digestibility and spatial distribution of cell wall polymers

Ulla Holopainen-Mantila, Kaisa Marjamaa, Zara Merali, Andres Käsper, Peter de Bot, Anna Stiina Jääskeläinen, Keith Waldron, Kristiina Kruus, Tarja Tamminen

Research output: Contribution to journalArticleScientificpeer-review

42 Citations (Scopus)

Abstract

The effect of hydrothermal pretreatment on chemical composition, microscopic structure and enzymatic digestibility of wheat straw was studied. Wheat straw was pretreated with increasing severity to obtain series of samples with altered chemistry and structure. The hydrothermal pretreatment caused solubilisation of arabinoxylan and phenolic acids and their dimers in a temperature dependent manner with minor effects on the cellulose and Klason lignin content. In the cell wall level, the pretreatment intensified staining of cellulose and relocalised xylan in the cell walls. The distribution, properties and content of the cell wall phenolic compounds was altered as observed with phloroglucinol and autofluorescence imaging. In the enzymatic hydrolysis, the highest yields were obtained from the samples with a low xylan and diferulate content. On the cell wall structural level, the sample with the highest digestibility was observed to have intensified cellulose staining, possibly reflecting the increased accessibility of cellulose.
Original languageEnglish
Pages (from-to)156-162
Number of pages7
JournalBioresource Technology
Volume138
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

digestibility
Cellulose
Spatial distribution
cellulose
Polymers
polymer
chemical composition
Cells
spatial distribution
Xylans
Straw
Chemical analysis
straw
wheat
Phloroglucinol
Enzymatic hydrolysis
Lignin
phenolic compound
solubilization
Dimers

Keywords

  • Hydrolysis
  • Hydrothermal pretreatment
  • Lignin
  • Phenolic acids
  • Xylan

Cite this

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title = "Impact of hydrothermal pre-treatment to chemical composition, enzymatic digestibility and spatial distribution of cell wall polymers",
abstract = "The effect of hydrothermal pretreatment on chemical composition, microscopic structure and enzymatic digestibility of wheat straw was studied. Wheat straw was pretreated with increasing severity to obtain series of samples with altered chemistry and structure. The hydrothermal pretreatment caused solubilisation of arabinoxylan and phenolic acids and their dimers in a temperature dependent manner with minor effects on the cellulose and Klason lignin content. In the cell wall level, the pretreatment intensified staining of cellulose and relocalised xylan in the cell walls. The distribution, properties and content of the cell wall phenolic compounds was altered as observed with phloroglucinol and autofluorescence imaging. In the enzymatic hydrolysis, the highest yields were obtained from the samples with a low xylan and diferulate content. On the cell wall structural level, the sample with the highest digestibility was observed to have intensified cellulose staining, possibly reflecting the increased accessibility of cellulose.",
keywords = "Hydrolysis, Hydrothermal pretreatment, Lignin, Phenolic acids, Xylan",
author = "Ulla Holopainen-Mantila and Kaisa Marjamaa and Zara Merali and Andres K{\"a}sper and {de Bot}, Peter and J{\"a}{\"a}skel{\"a}inen, {Anna Stiina} and Keith Waldron and Kristiina Kruus and Tarja Tamminen",
year = "2013",
doi = "10.1016/j.biortech.2013.03.152",
language = "English",
volume = "138",
pages = "156--162",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier",

}

Impact of hydrothermal pre-treatment to chemical composition, enzymatic digestibility and spatial distribution of cell wall polymers. / Holopainen-Mantila, Ulla; Marjamaa, Kaisa; Merali, Zara; Käsper, Andres; de Bot, Peter; Jääskeläinen, Anna Stiina; Waldron, Keith; Kruus, Kristiina; Tamminen, Tarja.

In: Bioresource Technology, Vol. 138, 2013, p. 156-162.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Impact of hydrothermal pre-treatment to chemical composition, enzymatic digestibility and spatial distribution of cell wall polymers

AU - Holopainen-Mantila, Ulla

AU - Marjamaa, Kaisa

AU - Merali, Zara

AU - Käsper, Andres

AU - de Bot, Peter

AU - Jääskeläinen, Anna Stiina

AU - Waldron, Keith

AU - Kruus, Kristiina

AU - Tamminen, Tarja

PY - 2013

Y1 - 2013

N2 - The effect of hydrothermal pretreatment on chemical composition, microscopic structure and enzymatic digestibility of wheat straw was studied. Wheat straw was pretreated with increasing severity to obtain series of samples with altered chemistry and structure. The hydrothermal pretreatment caused solubilisation of arabinoxylan and phenolic acids and their dimers in a temperature dependent manner with minor effects on the cellulose and Klason lignin content. In the cell wall level, the pretreatment intensified staining of cellulose and relocalised xylan in the cell walls. The distribution, properties and content of the cell wall phenolic compounds was altered as observed with phloroglucinol and autofluorescence imaging. In the enzymatic hydrolysis, the highest yields were obtained from the samples with a low xylan and diferulate content. On the cell wall structural level, the sample with the highest digestibility was observed to have intensified cellulose staining, possibly reflecting the increased accessibility of cellulose.

AB - The effect of hydrothermal pretreatment on chemical composition, microscopic structure and enzymatic digestibility of wheat straw was studied. Wheat straw was pretreated with increasing severity to obtain series of samples with altered chemistry and structure. The hydrothermal pretreatment caused solubilisation of arabinoxylan and phenolic acids and their dimers in a temperature dependent manner with minor effects on the cellulose and Klason lignin content. In the cell wall level, the pretreatment intensified staining of cellulose and relocalised xylan in the cell walls. The distribution, properties and content of the cell wall phenolic compounds was altered as observed with phloroglucinol and autofluorescence imaging. In the enzymatic hydrolysis, the highest yields were obtained from the samples with a low xylan and diferulate content. On the cell wall structural level, the sample with the highest digestibility was observed to have intensified cellulose staining, possibly reflecting the increased accessibility of cellulose.

KW - Hydrolysis

KW - Hydrothermal pretreatment

KW - Lignin

KW - Phenolic acids

KW - Xylan

U2 - 10.1016/j.biortech.2013.03.152

DO - 10.1016/j.biortech.2013.03.152

M3 - Article

VL - 138

SP - 156

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JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -