Impact of the Donnan effect on the action of xylanases on fibre substrates

Johanna Buchert (Corresponding Author), Tarja Tamminen, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)

Abstract

Carboxylic acid bound to xylans in the fibre matrix cause a pH gradient between the fibre and the surrounding solution, known as the Donnan effect. The gradient is dependent on the ionic strength of the fibre solution. When meta1-free kraft pulp was used as a substrate for the Trichoderma reesei xylanase, the apparent pH optimum of the xylanase at low ionic strengths was found to be significantly higher, around 9, when a monovalent hydroxide was used for pH adjustment, as compared to the pH optimum obtained with a divalent metal hydroxide, i.e. pH 5–6. By increasing the ionic strength of the pulp solution by salt addition, the pH optimae of the xylanase became 5–6 with all the hydroxides. This difference was caused by the Donnan effect. Thus, at low ionic strengths the actual pH is lower than that measured in the solution, resulting in an increased apparent pH optimum of the xylanase. In practice, these results are important when applying xylanases on practical, fibre bound substrates, such as kraft pulps.

Original languageEnglish
Pages (from-to)217 - 222
Number of pages6
JournalJournal of Biotechnology
Volume57
Issue number1 - 3
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

Fingerprint

Ionic strength
Fibers
Kraft pulp
Osmolar Concentration
Substrates
Hydroxides
Xylans
Carboxylic Acids
Carboxylic acids
Pulp
Salts
Metals
Trichoderma
Proton-Motive Force
hydroxide ion

Cite this

Buchert, Johanna ; Tamminen, Tarja ; Viikari, Liisa. / Impact of the Donnan effect on the action of xylanases on fibre substrates. In: Journal of Biotechnology. 1997 ; Vol. 57, No. 1 - 3. pp. 217 - 222.
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abstract = "Carboxylic acid bound to xylans in the fibre matrix cause a pH gradient between the fibre and the surrounding solution, known as the Donnan effect. The gradient is dependent on the ionic strength of the fibre solution. When meta1-free kraft pulp was used as a substrate for the Trichoderma reesei xylanase, the apparent pH optimum of the xylanase at low ionic strengths was found to be significantly higher, around 9, when a monovalent hydroxide was used for pH adjustment, as compared to the pH optimum obtained with a divalent metal hydroxide, i.e. pH 5–6. By increasing the ionic strength of the pulp solution by salt addition, the pH optimae of the xylanase became 5–6 with all the hydroxides. This difference was caused by the Donnan effect. Thus, at low ionic strengths the actual pH is lower than that measured in the solution, resulting in an increased apparent pH optimum of the xylanase. In practice, these results are important when applying xylanases on practical, fibre bound substrates, such as kraft pulps.",
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Impact of the Donnan effect on the action of xylanases on fibre substrates. / Buchert, Johanna (Corresponding Author); Tamminen, Tarja; Viikari, Liisa.

In: Journal of Biotechnology, Vol. 57, No. 1 - 3, 1997, p. 217 - 222.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Impact of the Donnan effect on the action of xylanases on fibre substrates

AU - Buchert, Johanna

AU - Tamminen, Tarja

AU - Viikari, Liisa

PY - 1997

Y1 - 1997

N2 - Carboxylic acid bound to xylans in the fibre matrix cause a pH gradient between the fibre and the surrounding solution, known as the Donnan effect. The gradient is dependent on the ionic strength of the fibre solution. When meta1-free kraft pulp was used as a substrate for the Trichoderma reesei xylanase, the apparent pH optimum of the xylanase at low ionic strengths was found to be significantly higher, around 9, when a monovalent hydroxide was used for pH adjustment, as compared to the pH optimum obtained with a divalent metal hydroxide, i.e. pH 5–6. By increasing the ionic strength of the pulp solution by salt addition, the pH optimae of the xylanase became 5–6 with all the hydroxides. This difference was caused by the Donnan effect. Thus, at low ionic strengths the actual pH is lower than that measured in the solution, resulting in an increased apparent pH optimum of the xylanase. In practice, these results are important when applying xylanases on practical, fibre bound substrates, such as kraft pulps.

AB - Carboxylic acid bound to xylans in the fibre matrix cause a pH gradient between the fibre and the surrounding solution, known as the Donnan effect. The gradient is dependent on the ionic strength of the fibre solution. When meta1-free kraft pulp was used as a substrate for the Trichoderma reesei xylanase, the apparent pH optimum of the xylanase at low ionic strengths was found to be significantly higher, around 9, when a monovalent hydroxide was used for pH adjustment, as compared to the pH optimum obtained with a divalent metal hydroxide, i.e. pH 5–6. By increasing the ionic strength of the pulp solution by salt addition, the pH optimae of the xylanase became 5–6 with all the hydroxides. This difference was caused by the Donnan effect. Thus, at low ionic strengths the actual pH is lower than that measured in the solution, resulting in an increased apparent pH optimum of the xylanase. In practice, these results are important when applying xylanases on practical, fibre bound substrates, such as kraft pulps.

U2 - 10.1016/S0168-1656(97)00101-6

DO - 10.1016/S0168-1656(97)00101-6

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JF - Journal of Biotechnology

SN - 0168-1656

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ER -