Impact of water content on the solubilisation of arabinoxylan during xylanase treatment of wheat bran

Outi Santala (Corresponding Author), Pekka Lehtinen, Emilia Nordlund, Tapani Suortti, Kaisa Poutanen

Research output: Contribution to journalArticleScientificpeer-review

42 Citations (Scopus)


Arabinoxylan (AX) has a major impact on the functional properties of wheat bran, and it has been shown that technological properties of bran can be improved by using endoxylanases. Enzymatic treatments are typically conducted at high water content. However, in industrial applications, low water content may be advantageous, especially when targeting dry end products. The aim of the study was to examine the impact of water content, ranging from 20 to 90%, on the efficiency of endoxylanase treatment of wheat bran. Interestingly, AX solubilisation was highest at the water contents of 40 and 90%. At water contents 50–80%, AX solubilisation was lower than at 40 and 90%. Furthermore, at low water content, less depolymerisation was detected. At water content of 40%, the bran-water mixture was transformed from powder-like into compact mass. Probably the compact consistency of the material enhanced AX solubilisation by increased breakdown of bran cell walls due to shear forces or via enhanced enzyme binding to the substrate. The results show that solubilisation of bran AX can also be efficiently performed at low water content.
Original languageEnglish
Pages (from-to)187-194
JournalJournal of Cereal Science
Issue number2
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed


  • Arabinoxylan
  • water content
  • wheat bran
  • xylanase


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