Abstract
| Original language | English |
|---|---|
| Title of host publication | 22nd Fungal Genetics Conference at Asilomar |
| Subtitle of host publication | Abstracts from the 2003 Fungal Genetics Conference at Asilomar |
| DOIs | |
| Publication status | Published - 2003 |
| Event | 22nd Fungal Genetics Conference - Asilomar, United States Duration: 18 Mar 2003 → 22 Mar 2003 |
Publication series
| Name | Fungal Genetics Reports |
|---|---|
| Publisher | New Prairie Press |
| Number | Article 18 |
| Volume | 50 |
Conference
| Conference | 22nd Fungal Genetics Conference |
|---|---|
| Country | United States |
| City | Asilomar |
| Period | 18/03/03 → 22/03/03 |
Fingerprint
Cite this
}
Improvement of foreign protein production by constitutive UPR induction in Aspergillus niger var. awamori. / Valkonen, Mari; Saloheimo, Markku; Ward, Michael; Penttilä, Merja.
22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar. 2003. 420 (Fungal Genetics Reports; No. Article 18, Vol. 50).Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Scientific
TY - CHAP
T1 - Improvement of foreign protein production by constitutive UPR induction in Aspergillus niger var. awamori
AU - Valkonen, Mari
AU - Saloheimo, Markku
AU - Ward, Michael
AU - Penttilä, Merja
PY - 2003
Y1 - 2003
N2 - A signalling pathway from ER to nucleus called the unfolded protein response (UPR) controls the expression of genes for several endoplasmic reticulum-resident (ER) chaperones and foldases. When the unfolded protein response pathway is active, a signal is transduced from the ER lumen to the transcription machinery in the nucleus. It has been shown that the yeast transcription factor mediating the UPR induction of the chaperone and foldase genes is Hac1p. The UPR-induced form of the A. niger var. awamori hacA cDNA was expressed in A. niger var. awamori strain producing Trametes versicolor laccase. For the overexpression of hacA, the induced form of the A. niger var.awamori hacA cDNA was first created by deleting the 20 bp intron and truncating the 5' flanking region by about 150 bp, which omitted the upstream open reading frame. The laccase activity measurements made from the supernatants show that all the transformants produce more laccase than the parental strain. The production levels of the transformants were in the fifth day samples 3 to 7.6 fold higher than in the parental strain. On the seventh day of cultivation the transformants produced 2 to 5.4 fold more laccase than the parental strain. This indicates that the overexpression of HACA protein induces the production of heterologous proteins in A. niger.
AB - A signalling pathway from ER to nucleus called the unfolded protein response (UPR) controls the expression of genes for several endoplasmic reticulum-resident (ER) chaperones and foldases. When the unfolded protein response pathway is active, a signal is transduced from the ER lumen to the transcription machinery in the nucleus. It has been shown that the yeast transcription factor mediating the UPR induction of the chaperone and foldase genes is Hac1p. The UPR-induced form of the A. niger var. awamori hacA cDNA was expressed in A. niger var. awamori strain producing Trametes versicolor laccase. For the overexpression of hacA, the induced form of the A. niger var.awamori hacA cDNA was first created by deleting the 20 bp intron and truncating the 5' flanking region by about 150 bp, which omitted the upstream open reading frame. The laccase activity measurements made from the supernatants show that all the transformants produce more laccase than the parental strain. The production levels of the transformants were in the fifth day samples 3 to 7.6 fold higher than in the parental strain. On the seventh day of cultivation the transformants produced 2 to 5.4 fold more laccase than the parental strain. This indicates that the overexpression of HACA protein induces the production of heterologous proteins in A. niger.
UR - http://newprairiepress.org/cgi/viewcontent.cgi?article=1164&context=fgr
U2 - 10.4148/1941-4765.1164
DO - 10.4148/1941-4765.1164
M3 - Conference abstract in proceedings
T3 - Fungal Genetics Reports
BT - 22nd Fungal Genetics Conference at Asilomar
ER -