Improvement of foreign protein production by constitutive UPR induction in Aspergillus niger var. awamori

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

A signalling pathway from ER to nucleus called the unfolded protein response (UPR) controls the expression of genes for several endoplasmic reticulum-resident (ER) chaperones and foldases. When the unfolded protein response pathway is active, a signal is transduced from the ER lumen to the transcription machinery in the nucleus. It has been shown that the yeast transcription factor mediating the UPR induction of the chaperone and foldase genes is Hac1p. The UPR-induced form of the A. niger var. awamori hacA cDNA was expressed in A. niger var. awamori strain producing Trametes versicolor laccase. For the overexpression of hacA, the induced form of the A. niger var.awamori hacA cDNA was first created by deleting the 20 bp intron and truncating the 5' flanking region by about 150 bp, which omitted the upstream open reading frame. The laccase activity measurements made from the supernatants show that all the transformants produce more laccase than the parental strain. The production levels of the transformants were in the fifth day samples 3 to 7.6 fold higher than in the parental strain. On the seventh day of cultivation the transformants produced 2 to 5.4 fold more laccase than the parental strain. This indicates that the overexpression of HACA protein induces the production of heterologous proteins in A. niger.
Original languageEnglish
Title of host publication22nd Fungal Genetics Conference at Asilomar
Subtitle of host publicationAbstracts from the 2003 Fungal Genetics Conference at Asilomar
DOIs
Publication statusPublished - 2003
Event22nd Fungal Genetics Conference - Asilomar, United States
Duration: 18 Mar 200322 Mar 2003

Publication series

NameFungal Genetics Reports
PublisherNew Prairie Press
NumberArticle 18
Volume50

Conference

Conference22nd Fungal Genetics Conference
CountryUnited States
CityAsilomar
Period18/03/0322/03/03

Fingerprint

unfolded protein response
laccase
Aspergillus niger
endoplasmic reticulum
proteins
Coriolus versicolor
open reading frames
introns
transcription factors
transcription (genetics)
yeasts
gene expression
genes
sampling

Cite this

Valkonen, M., Saloheimo, M., Ward, M., & Penttilä, M. (2003). Improvement of foreign protein production by constitutive UPR induction in Aspergillus niger var. awamori. In 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar [420] Fungal Genetics Reports, No. Article 18, Vol.. 50 https://doi.org/10.4148/1941-4765.1164
Valkonen, Mari ; Saloheimo, Markku ; Ward, Michael ; Penttilä, Merja. / Improvement of foreign protein production by constitutive UPR induction in Aspergillus niger var. awamori. 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar. 2003. (Fungal Genetics Reports; No. Article 18, Vol. 50).
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abstract = "A signalling pathway from ER to nucleus called the unfolded protein response (UPR) controls the expression of genes for several endoplasmic reticulum-resident (ER) chaperones and foldases. When the unfolded protein response pathway is active, a signal is transduced from the ER lumen to the transcription machinery in the nucleus. It has been shown that the yeast transcription factor mediating the UPR induction of the chaperone and foldase genes is Hac1p. The UPR-induced form of the A. niger var. awamori hacA cDNA was expressed in A. niger var. awamori strain producing Trametes versicolor laccase. For the overexpression of hacA, the induced form of the A. niger var.awamori hacA cDNA was first created by deleting the 20 bp intron and truncating the 5' flanking region by about 150 bp, which omitted the upstream open reading frame. The laccase activity measurements made from the supernatants show that all the transformants produce more laccase than the parental strain. The production levels of the transformants were in the fifth day samples 3 to 7.6 fold higher than in the parental strain. On the seventh day of cultivation the transformants produced 2 to 5.4 fold more laccase than the parental strain. This indicates that the overexpression of HACA protein induces the production of heterologous proteins in A. niger.",
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Valkonen, M, Saloheimo, M, Ward, M & Penttilä, M 2003, Improvement of foreign protein production by constitutive UPR induction in Aspergillus niger var. awamori. in 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar., 420, Fungal Genetics Reports, no. Article 18, vol. 50, 22nd Fungal Genetics Conference, Asilomar, United States, 18/03/03. https://doi.org/10.4148/1941-4765.1164

Improvement of foreign protein production by constitutive UPR induction in Aspergillus niger var. awamori. / Valkonen, Mari; Saloheimo, Markku; Ward, Michael; Penttilä, Merja.

22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar. 2003. 420 (Fungal Genetics Reports; No. Article 18, Vol. 50).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

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T1 - Improvement of foreign protein production by constitutive UPR induction in Aspergillus niger var. awamori

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Y1 - 2003

N2 - A signalling pathway from ER to nucleus called the unfolded protein response (UPR) controls the expression of genes for several endoplasmic reticulum-resident (ER) chaperones and foldases. When the unfolded protein response pathway is active, a signal is transduced from the ER lumen to the transcription machinery in the nucleus. It has been shown that the yeast transcription factor mediating the UPR induction of the chaperone and foldase genes is Hac1p. The UPR-induced form of the A. niger var. awamori hacA cDNA was expressed in A. niger var. awamori strain producing Trametes versicolor laccase. For the overexpression of hacA, the induced form of the A. niger var.awamori hacA cDNA was first created by deleting the 20 bp intron and truncating the 5' flanking region by about 150 bp, which omitted the upstream open reading frame. The laccase activity measurements made from the supernatants show that all the transformants produce more laccase than the parental strain. The production levels of the transformants were in the fifth day samples 3 to 7.6 fold higher than in the parental strain. On the seventh day of cultivation the transformants produced 2 to 5.4 fold more laccase than the parental strain. This indicates that the overexpression of HACA protein induces the production of heterologous proteins in A. niger.

AB - A signalling pathway from ER to nucleus called the unfolded protein response (UPR) controls the expression of genes for several endoplasmic reticulum-resident (ER) chaperones and foldases. When the unfolded protein response pathway is active, a signal is transduced from the ER lumen to the transcription machinery in the nucleus. It has been shown that the yeast transcription factor mediating the UPR induction of the chaperone and foldase genes is Hac1p. The UPR-induced form of the A. niger var. awamori hacA cDNA was expressed in A. niger var. awamori strain producing Trametes versicolor laccase. For the overexpression of hacA, the induced form of the A. niger var.awamori hacA cDNA was first created by deleting the 20 bp intron and truncating the 5' flanking region by about 150 bp, which omitted the upstream open reading frame. The laccase activity measurements made from the supernatants show that all the transformants produce more laccase than the parental strain. The production levels of the transformants were in the fifth day samples 3 to 7.6 fold higher than in the parental strain. On the seventh day of cultivation the transformants produced 2 to 5.4 fold more laccase than the parental strain. This indicates that the overexpression of HACA protein induces the production of heterologous proteins in A. niger.

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M3 - Conference abstract in proceedings

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BT - 22nd Fungal Genetics Conference at Asilomar

ER -

Valkonen M, Saloheimo M, Ward M, Penttilä M. Improvement of foreign protein production by constitutive UPR induction in Aspergillus niger var. awamori. In 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar. 2003. 420. (Fungal Genetics Reports; No. Article 18, Vol. 50). https://doi.org/10.4148/1941-4765.1164