TY - CHAP
T1 - Improvement of foreign protein production by constitutive UPR induction in Aspergillus niger var. awamori
AU - Valkonen, Mari
AU - Saloheimo, Markku
AU - Ward, Michael
AU - Penttilä, Merja
PY - 2003
Y1 - 2003
N2 - A signalling pathway from ER to nucleus called the unfolded protein
response (UPR) controls the expression of genes for several endoplasmic
reticulum-resident (ER) chaperones and foldases. When the unfolded protein
response pathway is active, a signal is transduced from the ER lumen to the
transcription machinery in the nucleus. It has been shown that the yeast
transcription factor mediating the UPR induction of the chaperone and foldase
genes is Hac1p. The UPR-induced form of the A. niger var. awamori hacA cDNA
was expressed in A. niger var. awamori strain producing Trametes versicolor
laccase. For the overexpression of hacA, the induced form of the A. niger
var.awamori hacA cDNA was first created by deleting the 20 bp intron and
truncating the 5' flanking region by about 150 bp, which omitted the upstream
open reading frame. The laccase activity measurements made from the
supernatants show that all the transformants produce more laccase than the
parental strain. The production levels of the transformants were in the fifth
day samples 3 to 7.6 fold higher than in the parental strain. On the seventh
day of cultivation the transformants produced 2 to 5.4 fold more laccase than
the parental strain. This indicates that the overexpression of HACA protein
induces the production of heterologous proteins in A. niger.
AB - A signalling pathway from ER to nucleus called the unfolded protein
response (UPR) controls the expression of genes for several endoplasmic
reticulum-resident (ER) chaperones and foldases. When the unfolded protein
response pathway is active, a signal is transduced from the ER lumen to the
transcription machinery in the nucleus. It has been shown that the yeast
transcription factor mediating the UPR induction of the chaperone and foldase
genes is Hac1p. The UPR-induced form of the A. niger var. awamori hacA cDNA
was expressed in A. niger var. awamori strain producing Trametes versicolor
laccase. For the overexpression of hacA, the induced form of the A. niger
var.awamori hacA cDNA was first created by deleting the 20 bp intron and
truncating the 5' flanking region by about 150 bp, which omitted the upstream
open reading frame. The laccase activity measurements made from the
supernatants show that all the transformants produce more laccase than the
parental strain. The production levels of the transformants were in the fifth
day samples 3 to 7.6 fold higher than in the parental strain. On the seventh
day of cultivation the transformants produced 2 to 5.4 fold more laccase than
the parental strain. This indicates that the overexpression of HACA protein
induces the production of heterologous proteins in A. niger.
UR - http://newprairiepress.org/cgi/viewcontent.cgi?article=1164&context=fgr
U2 - 10.4148/1941-4765.1164
DO - 10.4148/1941-4765.1164
M3 - Conference abstract in proceedings
T3 - Fungal Genetics Reports
BT - 22nd Fungal Genetics Conference at Asilomar
T2 - 22nd Fungal Genetics Conference
Y2 - 18 March 2003 through 22 March 2003
ER -