Improvement of malting quality of barley by complementing the malt enzyme spectrum

Anna-Maria Nuutila, Anneli Ritala, Marjatta Salmenkallio-Marttila, Kristian Aspegren, Reino Aikasalo, Ulrika Kurten, Jussi Tammisola, Teemu Teeri, Leena Mannonen, Veli Kauppinen

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

The processing quality of cereals can be modified by altering the structural grain constituents or the enzyme activities that mobilize storage reserves of the seeds. In order to complement the malt enzyme spectrum, a gene encoding for a thermotolerant fungal endo-(1,4)-ß-glucanase was introduced into two barley cultivars, Kymppi and Golden Promise. The gene was expressed in the seeds during germination, thus providing a thermotolerant enzyme that is active under mashing conditions. The amount of thermotolerant ß-glucanase produced by the seeds (ca. 0.025% soluble seed protein) has been shown to be sufficient to reduce wort viscosity by decreasing the soluble ß-glucan content. For the safe commercial cultivation of transgenic plants risk assessment of their cultivation is needed. In our study experimental estimates of the transgene flow from transgenic barley by pollen dispersal were produced. Field trials were conducted during the summers of 1996 and 1997. A transgenic barley line homozygous for the gene encoding for neomycin phosphotransferase was used as a source of pollen and male-sterile barley lines as recipients. In order to be able to transform the cross-fertilization frequencies to corresponding values of normal male-fertile barley, plots of normal barley were also included in the experimental plan. On the basis of our study, cross-fertilization in male-sterile recipient barley is possible with very low frequency up to 50 meters from the donor area. However, the frequency dramatically decreases with distance and due to self-pollination the possibility of cross-fertilization remains very low in normal cultivated barley.
Original languageEnglish
Pages (from-to)135-140
JournalPhytochemistry Reviews
Volume1
Issue number1
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

malting quality
malt
Hordeum
Quality Improvement
barley
Enzymes
enzymes
Seeds
fertilization (reproduction)
Fertilization
Pollen
seeds
Kanamycin Kinase
genetically modified organisms
Genes
pollen flow
Pollination
kanamycin kinase
genes
Glucans

Keywords

  • field trial
  • heterologous beta-glucanase
  • Hordeum vulgare
  • malting quality
  • risk assessment
  • transgenic barley

Cite this

Nuutila, A-M., Ritala, A., Salmenkallio-Marttila, M., Aspegren, K., Aikasalo, R., Kurten, U., ... Kauppinen, V. (2002). Improvement of malting quality of barley by complementing the malt enzyme spectrum. Phytochemistry Reviews, 1(1), 135-140. https://doi.org/10.1023/A:1015861125919
Nuutila, Anna-Maria ; Ritala, Anneli ; Salmenkallio-Marttila, Marjatta ; Aspegren, Kristian ; Aikasalo, Reino ; Kurten, Ulrika ; Tammisola, Jussi ; Teeri, Teemu ; Mannonen, Leena ; Kauppinen, Veli. / Improvement of malting quality of barley by complementing the malt enzyme spectrum. In: Phytochemistry Reviews. 2002 ; Vol. 1, No. 1. pp. 135-140.
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abstract = "The processing quality of cereals can be modified by altering the structural grain constituents or the enzyme activities that mobilize storage reserves of the seeds. In order to complement the malt enzyme spectrum, a gene encoding for a thermotolerant fungal endo-(1,4)-{\ss}-glucanase was introduced into two barley cultivars, Kymppi and Golden Promise. The gene was expressed in the seeds during germination, thus providing a thermotolerant enzyme that is active under mashing conditions. The amount of thermotolerant {\ss}-glucanase produced by the seeds (ca. 0.025{\%} soluble seed protein) has been shown to be sufficient to reduce wort viscosity by decreasing the soluble {\ss}-glucan content. For the safe commercial cultivation of transgenic plants risk assessment of their cultivation is needed. In our study experimental estimates of the transgene flow from transgenic barley by pollen dispersal were produced. Field trials were conducted during the summers of 1996 and 1997. A transgenic barley line homozygous for the gene encoding for neomycin phosphotransferase was used as a source of pollen and male-sterile barley lines as recipients. In order to be able to transform the cross-fertilization frequencies to corresponding values of normal male-fertile barley, plots of normal barley were also included in the experimental plan. On the basis of our study, cross-fertilization in male-sterile recipient barley is possible with very low frequency up to 50 meters from the donor area. However, the frequency dramatically decreases with distance and due to self-pollination the possibility of cross-fertilization remains very low in normal cultivated barley.",
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Nuutila, A-M, Ritala, A, Salmenkallio-Marttila, M, Aspegren, K, Aikasalo, R, Kurten, U, Tammisola, J, Teeri, T, Mannonen, L & Kauppinen, V 2002, 'Improvement of malting quality of barley by complementing the malt enzyme spectrum', Phytochemistry Reviews, vol. 1, no. 1, pp. 135-140. https://doi.org/10.1023/A:1015861125919

Improvement of malting quality of barley by complementing the malt enzyme spectrum. / Nuutila, Anna-Maria; Ritala, Anneli; Salmenkallio-Marttila, Marjatta; Aspegren, Kristian; Aikasalo, Reino; Kurten, Ulrika; Tammisola, Jussi; Teeri, Teemu; Mannonen, Leena; Kauppinen, Veli.

In: Phytochemistry Reviews, Vol. 1, No. 1, 2002, p. 135-140.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Improvement of malting quality of barley by complementing the malt enzyme spectrum

AU - Nuutila, Anna-Maria

AU - Ritala, Anneli

AU - Salmenkallio-Marttila, Marjatta

AU - Aspegren, Kristian

AU - Aikasalo, Reino

AU - Kurten, Ulrika

AU - Tammisola, Jussi

AU - Teeri, Teemu

AU - Mannonen, Leena

AU - Kauppinen, Veli

PY - 2002

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N2 - The processing quality of cereals can be modified by altering the structural grain constituents or the enzyme activities that mobilize storage reserves of the seeds. In order to complement the malt enzyme spectrum, a gene encoding for a thermotolerant fungal endo-(1,4)-ß-glucanase was introduced into two barley cultivars, Kymppi and Golden Promise. The gene was expressed in the seeds during germination, thus providing a thermotolerant enzyme that is active under mashing conditions. The amount of thermotolerant ß-glucanase produced by the seeds (ca. 0.025% soluble seed protein) has been shown to be sufficient to reduce wort viscosity by decreasing the soluble ß-glucan content. For the safe commercial cultivation of transgenic plants risk assessment of their cultivation is needed. In our study experimental estimates of the transgene flow from transgenic barley by pollen dispersal were produced. Field trials were conducted during the summers of 1996 and 1997. A transgenic barley line homozygous for the gene encoding for neomycin phosphotransferase was used as a source of pollen and male-sterile barley lines as recipients. In order to be able to transform the cross-fertilization frequencies to corresponding values of normal male-fertile barley, plots of normal barley were also included in the experimental plan. On the basis of our study, cross-fertilization in male-sterile recipient barley is possible with very low frequency up to 50 meters from the donor area. However, the frequency dramatically decreases with distance and due to self-pollination the possibility of cross-fertilization remains very low in normal cultivated barley.

AB - The processing quality of cereals can be modified by altering the structural grain constituents or the enzyme activities that mobilize storage reserves of the seeds. In order to complement the malt enzyme spectrum, a gene encoding for a thermotolerant fungal endo-(1,4)-ß-glucanase was introduced into two barley cultivars, Kymppi and Golden Promise. The gene was expressed in the seeds during germination, thus providing a thermotolerant enzyme that is active under mashing conditions. The amount of thermotolerant ß-glucanase produced by the seeds (ca. 0.025% soluble seed protein) has been shown to be sufficient to reduce wort viscosity by decreasing the soluble ß-glucan content. For the safe commercial cultivation of transgenic plants risk assessment of their cultivation is needed. In our study experimental estimates of the transgene flow from transgenic barley by pollen dispersal were produced. Field trials were conducted during the summers of 1996 and 1997. A transgenic barley line homozygous for the gene encoding for neomycin phosphotransferase was used as a source of pollen and male-sterile barley lines as recipients. In order to be able to transform the cross-fertilization frequencies to corresponding values of normal male-fertile barley, plots of normal barley were also included in the experimental plan. On the basis of our study, cross-fertilization in male-sterile recipient barley is possible with very low frequency up to 50 meters from the donor area. However, the frequency dramatically decreases with distance and due to self-pollination the possibility of cross-fertilization remains very low in normal cultivated barley.

KW - field trial

KW - heterologous beta-glucanase

KW - Hordeum vulgare

KW - malting quality

KW - risk assessment

KW - transgenic barley

U2 - 10.1023/A:1015861125919

DO - 10.1023/A:1015861125919

M3 - Article

VL - 1

SP - 135

EP - 140

JO - Phytochemistry Reviews

JF - Phytochemistry Reviews

SN - 1568-7767

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