Improving oat Beta-glucan content by biotechnological methods

E. Kiviharju, Anneli Ritala, P. Tanhuanpää, R. Kalendar, O. Manninen, Tapani Suortti, V. Hietaniemi, L. Pietilä, Kirsi-Marja Oksman-Caldentey, Anna-Maria Nuutila, A. Schulman

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Oat (Avena sativa L.) products lower the blood cholesterol level, a risk factor in heart diseases. Moreover, Beta-glucan also helps to normalize the postprandial blood glucose level and reduces the risk of colon cancer. These health benefits are mainly associated with high levels of mixed-linked (1,3)-(1,4)-Beta-D-glucan, a dietary fiber of which the main part is soluble. The amount of Beta-glucan is dependent on the genotype and thus can be increased by cultivar breeding. In this study, modern biotechnology tools were applied in order to breed high Beta-glucan oat cultivars for food and processing purposes. To understand the inheritance of Beta-glucan content in oat and to enable DNA-marker-assisted selection in oat cultivar breeding, a genetic linkage map was constructed for a Nordic oat cross. An Aslak x Matilda mapping population of 137 totally homozygous DH-lines was produced by anther culture. The linkage map consisted of over 600 PCR-based DNA-markers, including microsatellites, RAPDs, REMAPs, ISSRs, SRAPs and AFLPs. The QTL analysis showed two QTLs associated with Beta-glucan content. Together they explained about 37% of the variation in the DH lines. In both chromosomal regions alleles from Aslak had a favorable effect on Beta-glucan content. On the basis of the results, markers tightly linked to the genes themselves may be developed for DNA-marker assisted selection of high Beta-glucan content containing oat breeding lines. QTLs were located also for other traits of interest. Another aim was to modify the Beta-glucan content of Finnish oat cultivars through genetic engineering. Embryogenic cell cultures were started from mature embryos of oat cultivars Aslak, Veli, and Kolbu. A microbial 1,3-Beta-glucan synthase was transferred to oat cell lines by particle bombardment and transgenic plants were regenerated. The expression of the microbial gene in transgenic cell cultures was demonstrated by semi-quantitative RT-PCR. The 1,3-1,4-beta-glucan amounts were reduced and the molecular weight of the mixed-linked Beta-glucan differed in some transgenic seed lines when compared to non-transgenic control seeds. The analyses of 1,3-beta-glucan contents of transgenic cell lines and seeds is ongoing. A basis exists for modifying oat Beta-glucan contents through genetic engineering.
Original languageEnglish
Title of host publicationPlants for Human Health in the Post-Genome Era
Subtitle of host publicationPSE Congress
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages120-120
ISBN (Electronic)978-951-38-6322-7
ISBN (Print)978-951-38-6321-0
Publication statusPublished - 2007
MoE publication typeNot Eligible
EventPSE Congress: Plants for Human Health in the Post-Genome Era - Helsinki, Finland
Duration: 26 Aug 200729 Aug 2007

Publication series

SeriesVTT Symposium
Number249
ISSN0357-9387

Conference

ConferencePSE Congress: Plants for Human Health in the Post-Genome Era
CountryFinland
CityHelsinki
Period26/08/0729/08/07

Fingerprint

beta-glucans
oats
methodology
cultivars
quantitative trait loci
genetically modified organisms
genetic engineering
marker-assisted selection
genetic markers
chromosome mapping
cell culture
seeds
cell lines
1,3-beta-glucan synthase
microsatellite repeats
anther culture
breeding
heart diseases
Avena sativa
glucans

Cite this

Kiviharju, E., Ritala, A., Tanhuanpää, P., Kalendar, R., Manninen, O., Suortti, T., ... Schulman, A. (2007). Improving oat Beta-glucan content by biotechnological methods. In Plants for Human Health in the Post-Genome Era: PSE Congress (pp. 120-120). [D20] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 249
Kiviharju, E. ; Ritala, Anneli ; Tanhuanpää, P. ; Kalendar, R. ; Manninen, O. ; Suortti, Tapani ; Hietaniemi, V. ; Pietilä, L. ; Oksman-Caldentey, Kirsi-Marja ; Nuutila, Anna-Maria ; Schulman, A. / Improving oat Beta-glucan content by biotechnological methods. Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo : VTT Technical Research Centre of Finland, 2007. pp. 120-120 (VTT Symposium; No. 249).
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abstract = "Oat (Avena sativa L.) products lower the blood cholesterol level, a risk factor in heart diseases. Moreover, Beta-glucan also helps to normalize the postprandial blood glucose level and reduces the risk of colon cancer. These health benefits are mainly associated with high levels of mixed-linked (1,3)-(1,4)-Beta-D-glucan, a dietary fiber of which the main part is soluble. The amount of Beta-glucan is dependent on the genotype and thus can be increased by cultivar breeding. In this study, modern biotechnology tools were applied in order to breed high Beta-glucan oat cultivars for food and processing purposes. To understand the inheritance of Beta-glucan content in oat and to enable DNA-marker-assisted selection in oat cultivar breeding, a genetic linkage map was constructed for a Nordic oat cross. An Aslak x Matilda mapping population of 137 totally homozygous DH-lines was produced by anther culture. The linkage map consisted of over 600 PCR-based DNA-markers, including microsatellites, RAPDs, REMAPs, ISSRs, SRAPs and AFLPs. The QTL analysis showed two QTLs associated with Beta-glucan content. Together they explained about 37{\%} of the variation in the DH lines. In both chromosomal regions alleles from Aslak had a favorable effect on Beta-glucan content. On the basis of the results, markers tightly linked to the genes themselves may be developed for DNA-marker assisted selection of high Beta-glucan content containing oat breeding lines. QTLs were located also for other traits of interest. Another aim was to modify the Beta-glucan content of Finnish oat cultivars through genetic engineering. Embryogenic cell cultures were started from mature embryos of oat cultivars Aslak, Veli, and Kolbu. A microbial 1,3-Beta-glucan synthase was transferred to oat cell lines by particle bombardment and transgenic plants were regenerated. The expression of the microbial gene in transgenic cell cultures was demonstrated by semi-quantitative RT-PCR. The 1,3-1,4-beta-glucan amounts were reduced and the molecular weight of the mixed-linked Beta-glucan differed in some transgenic seed lines when compared to non-transgenic control seeds. The analyses of 1,3-beta-glucan contents of transgenic cell lines and seeds is ongoing. A basis exists for modifying oat Beta-glucan contents through genetic engineering.",
author = "E. Kiviharju and Anneli Ritala and P. Tanhuanp{\"a}{\"a} and R. Kalendar and O. Manninen and Tapani Suortti and V. Hietaniemi and L. Pietil{\"a} and Kirsi-Marja Oksman-Caldentey and Anna-Maria Nuutila and A. Schulman",
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Kiviharju, E, Ritala, A, Tanhuanpää, P, Kalendar, R, Manninen, O, Suortti, T, Hietaniemi, V, Pietilä, L, Oksman-Caldentey, K-M, Nuutila, A-M & Schulman, A 2007, Improving oat Beta-glucan content by biotechnological methods. in Plants for Human Health in the Post-Genome Era: PSE Congress., D20, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 249, pp. 120-120, PSE Congress: Plants for Human Health in the Post-Genome Era, Helsinki, Finland, 26/08/07.

Improving oat Beta-glucan content by biotechnological methods. / Kiviharju, E.; Ritala, Anneli; Tanhuanpää, P.; Kalendar, R.; Manninen, O.; Suortti, Tapani; Hietaniemi, V.; Pietilä, L.; Oksman-Caldentey, Kirsi-Marja; Nuutila, Anna-Maria; Schulman, A.

Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo : VTT Technical Research Centre of Finland, 2007. p. 120-120 D20 (VTT Symposium; No. 249).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Improving oat Beta-glucan content by biotechnological methods

AU - Kiviharju, E.

AU - Ritala, Anneli

AU - Tanhuanpää, P.

AU - Kalendar, R.

AU - Manninen, O.

AU - Suortti, Tapani

AU - Hietaniemi, V.

AU - Pietilä, L.

AU - Oksman-Caldentey, Kirsi-Marja

AU - Nuutila, Anna-Maria

AU - Schulman, A.

PY - 2007

Y1 - 2007

N2 - Oat (Avena sativa L.) products lower the blood cholesterol level, a risk factor in heart diseases. Moreover, Beta-glucan also helps to normalize the postprandial blood glucose level and reduces the risk of colon cancer. These health benefits are mainly associated with high levels of mixed-linked (1,3)-(1,4)-Beta-D-glucan, a dietary fiber of which the main part is soluble. The amount of Beta-glucan is dependent on the genotype and thus can be increased by cultivar breeding. In this study, modern biotechnology tools were applied in order to breed high Beta-glucan oat cultivars for food and processing purposes. To understand the inheritance of Beta-glucan content in oat and to enable DNA-marker-assisted selection in oat cultivar breeding, a genetic linkage map was constructed for a Nordic oat cross. An Aslak x Matilda mapping population of 137 totally homozygous DH-lines was produced by anther culture. The linkage map consisted of over 600 PCR-based DNA-markers, including microsatellites, RAPDs, REMAPs, ISSRs, SRAPs and AFLPs. The QTL analysis showed two QTLs associated with Beta-glucan content. Together they explained about 37% of the variation in the DH lines. In both chromosomal regions alleles from Aslak had a favorable effect on Beta-glucan content. On the basis of the results, markers tightly linked to the genes themselves may be developed for DNA-marker assisted selection of high Beta-glucan content containing oat breeding lines. QTLs were located also for other traits of interest. Another aim was to modify the Beta-glucan content of Finnish oat cultivars through genetic engineering. Embryogenic cell cultures were started from mature embryos of oat cultivars Aslak, Veli, and Kolbu. A microbial 1,3-Beta-glucan synthase was transferred to oat cell lines by particle bombardment and transgenic plants were regenerated. The expression of the microbial gene in transgenic cell cultures was demonstrated by semi-quantitative RT-PCR. The 1,3-1,4-beta-glucan amounts were reduced and the molecular weight of the mixed-linked Beta-glucan differed in some transgenic seed lines when compared to non-transgenic control seeds. The analyses of 1,3-beta-glucan contents of transgenic cell lines and seeds is ongoing. A basis exists for modifying oat Beta-glucan contents through genetic engineering.

AB - Oat (Avena sativa L.) products lower the blood cholesterol level, a risk factor in heart diseases. Moreover, Beta-glucan also helps to normalize the postprandial blood glucose level and reduces the risk of colon cancer. These health benefits are mainly associated with high levels of mixed-linked (1,3)-(1,4)-Beta-D-glucan, a dietary fiber of which the main part is soluble. The amount of Beta-glucan is dependent on the genotype and thus can be increased by cultivar breeding. In this study, modern biotechnology tools were applied in order to breed high Beta-glucan oat cultivars for food and processing purposes. To understand the inheritance of Beta-glucan content in oat and to enable DNA-marker-assisted selection in oat cultivar breeding, a genetic linkage map was constructed for a Nordic oat cross. An Aslak x Matilda mapping population of 137 totally homozygous DH-lines was produced by anther culture. The linkage map consisted of over 600 PCR-based DNA-markers, including microsatellites, RAPDs, REMAPs, ISSRs, SRAPs and AFLPs. The QTL analysis showed two QTLs associated with Beta-glucan content. Together they explained about 37% of the variation in the DH lines. In both chromosomal regions alleles from Aslak had a favorable effect on Beta-glucan content. On the basis of the results, markers tightly linked to the genes themselves may be developed for DNA-marker assisted selection of high Beta-glucan content containing oat breeding lines. QTLs were located also for other traits of interest. Another aim was to modify the Beta-glucan content of Finnish oat cultivars through genetic engineering. Embryogenic cell cultures were started from mature embryos of oat cultivars Aslak, Veli, and Kolbu. A microbial 1,3-Beta-glucan synthase was transferred to oat cell lines by particle bombardment and transgenic plants were regenerated. The expression of the microbial gene in transgenic cell cultures was demonstrated by semi-quantitative RT-PCR. The 1,3-1,4-beta-glucan amounts were reduced and the molecular weight of the mixed-linked Beta-glucan differed in some transgenic seed lines when compared to non-transgenic control seeds. The analyses of 1,3-beta-glucan contents of transgenic cell lines and seeds is ongoing. A basis exists for modifying oat Beta-glucan contents through genetic engineering.

M3 - Conference abstract in proceedings

SN - 978-951-38-6321-0

T3 - VTT Symposium

SP - 120

EP - 120

BT - Plants for Human Health in the Post-Genome Era

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Kiviharju E, Ritala A, Tanhuanpää P, Kalendar R, Manninen O, Suortti T et al. Improving oat Beta-glucan content by biotechnological methods. In Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo: VTT Technical Research Centre of Finland. 2007. p. 120-120. D20. (VTT Symposium; No. 249).