Myceligenerans crystallogenes sp. nov., isolated from Roman catacombs

Ingrid Groth (Corresponding Author), Peter Schumann, Barbara Schütze, Juan M. Gonzalez, Leonila Laiz, Maija-Liisa Suihko, Erko Stackebrandt

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

Three xylan-degrading actinobacterial strains were isolated from different sampling sites in the Roman catacombs of Domitilla and San Callisto. The organisms showed morphological and chemotaxonomic properties such as peptidoglycan type A4α, l-lys–l-thr–d-Glu; whole-cell sugars (glucose, mannose and galactose); octa-, hexa- and tetrahydrogenated menaquinones with nine isoprene units; phosphatidylglycerol and diphosphatidylglycerol as the major phospholipids; anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids; and a DNA G+C content of 72 mol%. These features are consistent with affiliation of these isolates to the genus Myceligenerans. The three isolates shared a 16S rRNA gene similarity of 99·9 % and were most closely related to Myceligenerans xiligouense DSM 15700T (97·9 % sequence similarity). The low level of DNA–DNA relatedness (about 14 %) and the differences in phenotypic characteristics between the novel strains and M. xiligouense DSM 15700T justify the proposal of a novel species of the genus Myceligenerans, Myceligenerans crystallogenes sp. nov., with CD12E2-27T (=HKI 0369T=DSM 17134T=NCIMB 14061T=VTT E-032285T) as the type strain.
Original languageEnglish
Pages (from-to)283-287
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume56
Issue number1
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

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Callisto
Vitamin K 2
Xylans
Phosphatidylglycerols
Cardiolipins
Peptidoglycan
isoprene
Base Composition
Mannose
phospholipid
Galactose
rRNA Genes
relatedness
Phospholipids
sugar
glucose
Fatty Acids
fatty acid
DNA
Glucose

Keywords

  • xylan-degrading actinobacterial strains

Cite this

Groth, I., Schumann, P., Schütze, B., Gonzalez, J. M., Laiz, L., Suihko, M-L., & Stackebrandt, E. (2006). Myceligenerans crystallogenes sp. nov., isolated from Roman catacombs. International Journal of Systematic and Evolutionary Microbiology, 56(1), 283-287. https://doi.org/10.1099/ijs.0.63756-0
Groth, Ingrid ; Schumann, Peter ; Schütze, Barbara ; Gonzalez, Juan M. ; Laiz, Leonila ; Suihko, Maija-Liisa ; Stackebrandt, Erko. / Myceligenerans crystallogenes sp. nov., isolated from Roman catacombs. In: International Journal of Systematic and Evolutionary Microbiology. 2006 ; Vol. 56, No. 1. pp. 283-287.
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abstract = "Three xylan-degrading actinobacterial strains were isolated from different sampling sites in the Roman catacombs of Domitilla and San Callisto. The organisms showed morphological and chemotaxonomic properties such as peptidoglycan type A4α, l-lys–l-thr–d-Glu; whole-cell sugars (glucose, mannose and galactose); octa-, hexa- and tetrahydrogenated menaquinones with nine isoprene units; phosphatidylglycerol and diphosphatidylglycerol as the major phospholipids; anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids; and a DNA G+C content of 72 mol{\%}. These features are consistent with affiliation of these isolates to the genus Myceligenerans. The three isolates shared a 16S rRNA gene similarity of 99·9 {\%} and were most closely related to Myceligenerans xiligouense DSM 15700T (97·9 {\%} sequence similarity). The low level of DNA–DNA relatedness (about 14 {\%}) and the differences in phenotypic characteristics between the novel strains and M. xiligouense DSM 15700T justify the proposal of a novel species of the genus Myceligenerans, Myceligenerans crystallogenes sp. nov., with CD12E2-27T (=HKI 0369T=DSM 17134T=NCIMB 14061T=VTT E-032285T) as the type strain.",
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Groth, I, Schumann, P, Schütze, B, Gonzalez, JM, Laiz, L, Suihko, M-L & Stackebrandt, E 2006, 'Myceligenerans crystallogenes sp. nov., isolated from Roman catacombs', International Journal of Systematic and Evolutionary Microbiology, vol. 56, no. 1, pp. 283-287. https://doi.org/10.1099/ijs.0.63756-0

Myceligenerans crystallogenes sp. nov., isolated from Roman catacombs. / Groth, Ingrid (Corresponding Author); Schumann, Peter; Schütze, Barbara; Gonzalez, Juan M.; Laiz, Leonila; Suihko, Maija-Liisa; Stackebrandt, Erko.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 56, No. 1, 2006, p. 283-287.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Myceligenerans crystallogenes sp. nov., isolated from Roman catacombs

AU - Groth, Ingrid

AU - Schumann, Peter

AU - Schütze, Barbara

AU - Gonzalez, Juan M.

AU - Laiz, Leonila

AU - Suihko, Maija-Liisa

AU - Stackebrandt, Erko

PY - 2006

Y1 - 2006

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AB - Three xylan-degrading actinobacterial strains were isolated from different sampling sites in the Roman catacombs of Domitilla and San Callisto. The organisms showed morphological and chemotaxonomic properties such as peptidoglycan type A4α, l-lys–l-thr–d-Glu; whole-cell sugars (glucose, mannose and galactose); octa-, hexa- and tetrahydrogenated menaquinones with nine isoprene units; phosphatidylglycerol and diphosphatidylglycerol as the major phospholipids; anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids; and a DNA G+C content of 72 mol%. These features are consistent with affiliation of these isolates to the genus Myceligenerans. The three isolates shared a 16S rRNA gene similarity of 99·9 % and were most closely related to Myceligenerans xiligouense DSM 15700T (97·9 % sequence similarity). The low level of DNA–DNA relatedness (about 14 %) and the differences in phenotypic characteristics between the novel strains and M. xiligouense DSM 15700T justify the proposal of a novel species of the genus Myceligenerans, Myceligenerans crystallogenes sp. nov., with CD12E2-27T (=HKI 0369T=DSM 17134T=NCIMB 14061T=VTT E-032285T) as the type strain.

KW - xylan-degrading actinobacterial strains

U2 - 10.1099/ijs.0.63756-0

DO - 10.1099/ijs.0.63756-0

M3 - Article

VL - 56

SP - 283

EP - 287

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 1

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