In situ Quartz crystal microbalance monitoring of Fab'-fragment binding to linker lipids in a phoshatidylcholine monolayer matrix

Application to immunosensors

Inger Vikholm (Corresponding Author), Martin Albers, Hannu Välimäki, Hannu Helle

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

Linker lipids were embedded in a phosphatidylcholine monolayer matrix prepared at the air–water interface.
The covalent coupling of antibody fragments, non-specific adsorption of bovine serum albumin and specific binding of antibodies was monitored in situ with a 10-MHz quartz crystal microbalance.
The attachment of antibody fragments and the activity of the layers was also showed with standardized radioimmunoassay.
The results demonstrate that the coupling of Fab′-fragments to linker lipids in a monolayer matrix is a promising approach to achieve a highly oriented layer of antibody fragments with a high density of binding sites on the sensor surface for immunological measurements.
Original languageEnglish
Pages (from-to)643-646
JournalThin Solid Films
Volume327-329
DOIs
Publication statusPublished - 1998
MoE publication typeNot Eligible

Fingerprint

Immunosensors
Immunoglobulin Fragments
Immunoglobulin Fab Fragments
Quartz crystal microbalances
quartz crystals
antibodies
Antibodies
microbalances
Lipids
lipids
Monolayers
fragments
Monitoring
matrices
radioimmunoassay
Bovine Serum Albumin
Phosphatidylcholines
Binding sites
albumins
Binding Sites

Cite this

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title = "In situ Quartz crystal microbalance monitoring of Fab'-fragment binding to linker lipids in a phoshatidylcholine monolayer matrix: Application to immunosensors",
abstract = "Linker lipids were embedded in a phosphatidylcholine monolayer matrix prepared at the air–water interface. The covalent coupling of antibody fragments, non-specific adsorption of bovine serum albumin and specific binding of antibodies was monitored in situ with a 10-MHz quartz crystal microbalance. The attachment of antibody fragments and the activity of the layers was also showed with standardized radioimmunoassay. The results demonstrate that the coupling of Fab′-fragments to linker lipids in a monolayer matrix is a promising approach to achieve a highly oriented layer of antibody fragments with a high density of binding sites on the sensor surface for immunological measurements.",
author = "Inger Vikholm and Martin Albers and Hannu V{\"a}lim{\"a}ki and Hannu Helle",
note = "Project code: K8SU00019",
year = "1998",
doi = "10.1016/S0040-6090(98)00731-7",
language = "English",
volume = "327-329",
pages = "643--646",
journal = "Thin Solid Films",
issn = "0040-6090",
publisher = "Elsevier",

}

In situ Quartz crystal microbalance monitoring of Fab'-fragment binding to linker lipids in a phoshatidylcholine monolayer matrix : Application to immunosensors. / Vikholm, Inger (Corresponding Author); Albers, Martin; Välimäki, Hannu; Helle, Hannu.

In: Thin Solid Films, Vol. 327-329, 1998, p. 643-646.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - In situ Quartz crystal microbalance monitoring of Fab'-fragment binding to linker lipids in a phoshatidylcholine monolayer matrix

T2 - Application to immunosensors

AU - Vikholm, Inger

AU - Albers, Martin

AU - Välimäki, Hannu

AU - Helle, Hannu

N1 - Project code: K8SU00019

PY - 1998

Y1 - 1998

N2 - Linker lipids were embedded in a phosphatidylcholine monolayer matrix prepared at the air–water interface. The covalent coupling of antibody fragments, non-specific adsorption of bovine serum albumin and specific binding of antibodies was monitored in situ with a 10-MHz quartz crystal microbalance. The attachment of antibody fragments and the activity of the layers was also showed with standardized radioimmunoassay. The results demonstrate that the coupling of Fab′-fragments to linker lipids in a monolayer matrix is a promising approach to achieve a highly oriented layer of antibody fragments with a high density of binding sites on the sensor surface for immunological measurements.

AB - Linker lipids were embedded in a phosphatidylcholine monolayer matrix prepared at the air–water interface. The covalent coupling of antibody fragments, non-specific adsorption of bovine serum albumin and specific binding of antibodies was monitored in situ with a 10-MHz quartz crystal microbalance. The attachment of antibody fragments and the activity of the layers was also showed with standardized radioimmunoassay. The results demonstrate that the coupling of Fab′-fragments to linker lipids in a monolayer matrix is a promising approach to achieve a highly oriented layer of antibody fragments with a high density of binding sites on the sensor surface for immunological measurements.

U2 - 10.1016/S0040-6090(98)00731-7

DO - 10.1016/S0040-6090(98)00731-7

M3 - Article

VL - 327-329

SP - 643

EP - 646

JO - Thin Solid Films

JF - Thin Solid Films

SN - 0040-6090

ER -