In vitro and in vivo gastrointestinal survival, antibiotic susceptibility and genetic identification of Propionibacterium freudenreichii ssp. shermanii JS

Tarja Suomalainen (Corresponding Author), Pia Sigvart-Mattila, Jaana Mättö, Soile Tynkkynen

Research output: Contribution to journalArticleScientificpeer-review

23 Citations (Scopus)

Abstract

The in vitro tolerance of Propionibacterium freudenreichii strains to low pH and high bile concentration, their antibiotic susceptibility and enzyme activity profiles were assessed. The gastrointestinal survival of the P. freudenreichii ssp. shermanii JS strain was also examined when administered to healthy adult volunteers in whey-based fruit juice. For tracking the strain JS in faecal samples, a genetic identification method for the strain was developed. No loss of viability was observed in any of the P. freudenreichii strains during the three-hour treatment at pH 4 or 3, whereas at pH 2 the cell counts of all strains decreased remarkably. Bile salt at 0.5% concentration had no apparent effect on the viability of the strains. No atypical antibiotic resistance was detected. P. freudenreichii ssp. shermanii JS strain was recovered in high numbers in faecal samples during the consumption of whey-based fruit juice enriched with the JS strain, and with few exceptions, it was not detected after the 2-week follow-up period.
Original languageEnglish
Pages (from-to)271 - 278
Number of pages8
JournalInternational Dairy Journal
Volume18
Issue number3
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Propionibacterium freudenreichii
Genetic Predisposition to Disease
antibiotics
Anti-Bacterial Agents
Microbial Drug Resistance
Bile Acids and Salts
Bile
fruit juices
whey
Healthy Volunteers
Cell Count
viability
In Vitro Techniques
Enzymes
bile salts
bile
antibiotic resistance
volunteers
enzyme activity

Keywords

  • Dairy propionibacteria
  • Probiotics
  • Gastric and intestinal tolerance

Cite this

Suomalainen, Tarja ; Sigvart-Mattila, Pia ; Mättö, Jaana ; Tynkkynen, Soile. / In vitro and in vivo gastrointestinal survival, antibiotic susceptibility and genetic identification of Propionibacterium freudenreichii ssp. shermanii JS. In: International Dairy Journal. 2008 ; Vol. 18, No. 3. pp. 271 - 278.
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abstract = "The in vitro tolerance of Propionibacterium freudenreichii strains to low pH and high bile concentration, their antibiotic susceptibility and enzyme activity profiles were assessed. The gastrointestinal survival of the P. freudenreichii ssp. shermanii JS strain was also examined when administered to healthy adult volunteers in whey-based fruit juice. For tracking the strain JS in faecal samples, a genetic identification method for the strain was developed. No loss of viability was observed in any of the P. freudenreichii strains during the three-hour treatment at pH 4 or 3, whereas at pH 2 the cell counts of all strains decreased remarkably. Bile salt at 0.5{\%} concentration had no apparent effect on the viability of the strains. No atypical antibiotic resistance was detected. P. freudenreichii ssp. shermanii JS strain was recovered in high numbers in faecal samples during the consumption of whey-based fruit juice enriched with the JS strain, and with few exceptions, it was not detected after the 2-week follow-up period.",
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In vitro and in vivo gastrointestinal survival, antibiotic susceptibility and genetic identification of Propionibacterium freudenreichii ssp. shermanii JS. / Suomalainen, Tarja (Corresponding Author); Sigvart-Mattila, Pia; Mättö, Jaana; Tynkkynen, Soile.

In: International Dairy Journal, Vol. 18, No. 3, 2008, p. 271 - 278.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - In vitro and in vivo gastrointestinal survival, antibiotic susceptibility and genetic identification of Propionibacterium freudenreichii ssp. shermanii JS

AU - Suomalainen, Tarja

AU - Sigvart-Mattila, Pia

AU - Mättö, Jaana

AU - Tynkkynen, Soile

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AB - The in vitro tolerance of Propionibacterium freudenreichii strains to low pH and high bile concentration, their antibiotic susceptibility and enzyme activity profiles were assessed. The gastrointestinal survival of the P. freudenreichii ssp. shermanii JS strain was also examined when administered to healthy adult volunteers in whey-based fruit juice. For tracking the strain JS in faecal samples, a genetic identification method for the strain was developed. No loss of viability was observed in any of the P. freudenreichii strains during the three-hour treatment at pH 4 or 3, whereas at pH 2 the cell counts of all strains decreased remarkably. Bile salt at 0.5% concentration had no apparent effect on the viability of the strains. No atypical antibiotic resistance was detected. P. freudenreichii ssp. shermanii JS strain was recovered in high numbers in faecal samples during the consumption of whey-based fruit juice enriched with the JS strain, and with few exceptions, it was not detected after the 2-week follow-up period.

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