In Vitro Characterization of Lactobacillus Strains Isolated from Fruit Processing By-Products as Potential Probiotics

Thatyane Mariano Rodrigues de Albuquerque, Estefânia Fernandes Garcia, Amanda de Oliveira Araújo, Marciane Magnani, Maria Saarela, Evandro Leite de Souza

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Nine wild Lactobacillus strains, namely Lactobacillus plantarum 53, Lactobacillus fermentum 56, L. fermentum 60, Lactobacillus paracasei 106, L. fermentum 250, L. fermentum 263, L. fermentum 139, L. fermentum 141, and L. fermentum 296, isolated from fruit processing by-products were evaluated in vitro for a series of safety, physiological functionality, and technological properties that could enable their use as probiotics. Considering the safety aspects, the resistance to antibiotics varied among the examined strains, and none of the strains presented hemolytic and mucinolytic activity. Regarding the physiological functionality properties, none of the strains were able to deconjugate bile salts; all of them presented low to moderate cell hydrophobicity and were able to autoaggregate, coaggregate with Listeria monocytogenes and Escherichia coli, and antagonize pathogenic bacteria. Exposure to pH 2 sharply decreased the survival of the examined strains after 1- or 2-h exposure; variable decreases were noted after 3-h exposure to pH 3. Overall, exposure to pH 5 and to bile salts (0.15, 0.3, and 1%) did not decrease the strains' survival. Examined strains presented better ability to survive from the exposure to simulated gastrointestinal conditions in laboratorial media and milk than in grape juice. Considering the technological properties, all the strains were positive for proteolytic activity and EPS and diacetyl production, and most of them had good tolerance to 1-4% NaCl. These results indicate that wild Lactobacillus strains isolated from fruit processing by-products could present performance compatible with probiotic properties and technological features that enable the development of probiotic foods with distinct characteristics.
Original languageEnglish
Pages (from-to)704-716
Number of pages13
JournalProbiotics and Antimicrobial Proteins
Volume10
Issue number4
Early online date2017
DOIs
Publication statusPublished - 2018
MoE publication typeNot Eligible

Fingerprint

Lactobacillus fermentum
Probiotics
Lactobacillus
Fruit
Bile Acids and Salts
Safety
Diacetyl
Lactobacillus plantarum
Vitis
Listeria monocytogenes
Microbial Drug Resistance
In Vitro Techniques
Hydrophobic and Hydrophilic Interactions
Milk
Escherichia coli
Bacteria
Food

Keywords

  • agroindustrial by-products
  • fruit
  • Lactobacillus
  • probiotic use

Cite this

de Albuquerque, T. M. R., Garcia, E. F., de Oliveira Araújo, A., Magnani, M., Saarela, M., & de Souza, E. L. (2018). In Vitro Characterization of Lactobacillus Strains Isolated from Fruit Processing By-Products as Potential Probiotics. Probiotics and Antimicrobial Proteins, 10(4), 704-716. https://doi.org/10.1007/s12602-017-9318-2
de Albuquerque, Thatyane Mariano Rodrigues ; Garcia, Estefânia Fernandes ; de Oliveira Araújo, Amanda ; Magnani, Marciane ; Saarela, Maria ; de Souza, Evandro Leite. / In Vitro Characterization of Lactobacillus Strains Isolated from Fruit Processing By-Products as Potential Probiotics. In: Probiotics and Antimicrobial Proteins. 2018 ; Vol. 10, No. 4. pp. 704-716.
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abstract = "Nine wild Lactobacillus strains, namely Lactobacillus plantarum 53, Lactobacillus fermentum 56, L. fermentum 60, Lactobacillus paracasei 106, L. fermentum 250, L. fermentum 263, L. fermentum 139, L. fermentum 141, and L. fermentum 296, isolated from fruit processing by-products were evaluated in vitro for a series of safety, physiological functionality, and technological properties that could enable their use as probiotics. Considering the safety aspects, the resistance to antibiotics varied among the examined strains, and none of the strains presented hemolytic and mucinolytic activity. Regarding the physiological functionality properties, none of the strains were able to deconjugate bile salts; all of them presented low to moderate cell hydrophobicity and were able to autoaggregate, coaggregate with Listeria monocytogenes and Escherichia coli, and antagonize pathogenic bacteria. Exposure to pH 2 sharply decreased the survival of the examined strains after 1- or 2-h exposure; variable decreases were noted after 3-h exposure to pH 3. Overall, exposure to pH 5 and to bile salts (0.15, 0.3, and 1{\%}) did not decrease the strains' survival. Examined strains presented better ability to survive from the exposure to simulated gastrointestinal conditions in laboratorial media and milk than in grape juice. Considering the technological properties, all the strains were positive for proteolytic activity and EPS and diacetyl production, and most of them had good tolerance to 1-4{\%} NaCl. These results indicate that wild Lactobacillus strains isolated from fruit processing by-products could present performance compatible with probiotic properties and technological features that enable the development of probiotic foods with distinct characteristics.",
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de Albuquerque, TMR, Garcia, EF, de Oliveira Araújo, A, Magnani, M, Saarela, M & de Souza, EL 2018, 'In Vitro Characterization of Lactobacillus Strains Isolated from Fruit Processing By-Products as Potential Probiotics', Probiotics and Antimicrobial Proteins, vol. 10, no. 4, pp. 704-716. https://doi.org/10.1007/s12602-017-9318-2

In Vitro Characterization of Lactobacillus Strains Isolated from Fruit Processing By-Products as Potential Probiotics. / de Albuquerque, Thatyane Mariano Rodrigues; Garcia, Estefânia Fernandes; de Oliveira Araújo, Amanda; Magnani, Marciane; Saarela, Maria; de Souza, Evandro Leite.

In: Probiotics and Antimicrobial Proteins, Vol. 10, No. 4, 2018, p. 704-716.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - In Vitro Characterization of Lactobacillus Strains Isolated from Fruit Processing By-Products as Potential Probiotics

AU - de Albuquerque, Thatyane Mariano Rodrigues

AU - Garcia, Estefânia Fernandes

AU - de Oliveira Araújo, Amanda

AU - Magnani, Marciane

AU - Saarela, Maria

AU - de Souza, Evandro Leite

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KW - agroindustrial by-products

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