Abstract
A protocol for the in vitro germination and the
establishment of tissue cultures of Bulbine caulescens
Linn., Kniphofia pumila Kunth, and K. uvaria Hook has
been developed. The members of the family Asphodelaceae
contain interesting substances with antiplasmodial
activity against several strains of Plasmodium
falciparum. With respect to species of Bulbine, this is
the first time that in vitro germination has been
achieved. For seeds of K. pumila and K. uvaria, a
facilitated germination was attained by an optimized
pretreatment of the seeds. After in vitro germination,
root explants of Kniphofia and bulb explants of B.
caulescens were successfully transformed into callus
cultures. From these, liquid cultures of the two species
of Kniphofia were easily produced. Studies on shoot
multiplication of Kniphofia in vitro were also performed.
Original language | English |
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Pages (from-to) | 125-129 |
Journal | Plant Cell Reports |
Volume | 21 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2002 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Bulbine caulescens; Kniphofia pumila; Kniphofia uvaria; In vitro germination