In vitro germination and establishment of tissue cultures of Bulbine caulescens and of two Kniphofia species (Asphodelaceae)

G. Bringmann, T. Noll, Heiko Rischer

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)

Abstract

A protocol for the in vitro germination and the establishment of tissue cultures of Bulbine caulescens Linn., Kniphofia pumila Kunth, and K. uvaria Hook has been developed. The members of the family Asphodelaceae contain interesting substances with antiplasmodial activity against several strains of Plasmodium falciparum. With respect to species of Bulbine, this is the first time that in vitro germination has been achieved. For seeds of K. pumila and K. uvaria, a facilitated germination was attained by an optimized pretreatment of the seeds. After in vitro germination, root explants of Kniphofia and bulb explants of B. caulescens were successfully transformed into callus cultures. From these, liquid cultures of the two species of Kniphofia were easily produced. Studies on shoot multiplication of Kniphofia in vitro were also performed.
Original languageEnglish
Pages (from-to)125-129
JournalPlant Cell Reports
Volume21
Issue number2
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Kniphofia
Xanthorrhoeaceae
tissue culture
Uvaria
germination
explants
antimalarials
Plasmodium falciparum
callus culture
seeds
bulbs
pretreatment
shoots
liquids

Keywords

  • Bulbine caulescens; Kniphofia pumila; Kniphofia uvaria; In vitro germination

Cite this

@article{9899a4885ba64c518f9734cfe01288a1,
title = "In vitro germination and establishment of tissue cultures of Bulbine caulescens and of two Kniphofia species (Asphodelaceae)",
abstract = "A protocol for the in vitro germination and the establishment of tissue cultures of Bulbine caulescens Linn., Kniphofia pumila Kunth, and K. uvaria Hook has been developed. The members of the family Asphodelaceae contain interesting substances with antiplasmodial activity against several strains of Plasmodium falciparum. With respect to species of Bulbine, this is the first time that in vitro germination has been achieved. For seeds of K. pumila and K. uvaria, a facilitated germination was attained by an optimized pretreatment of the seeds. After in vitro germination, root explants of Kniphofia and bulb explants of B. caulescens were successfully transformed into callus cultures. From these, liquid cultures of the two species of Kniphofia were easily produced. Studies on shoot multiplication of Kniphofia in vitro were also performed.",
keywords = "Bulbine caulescens; Kniphofia pumila; Kniphofia uvaria; In vitro germination",
author = "G. Bringmann and T. Noll and Heiko Rischer",
year = "2002",
doi = "10.1007/s00299-002-0497-1",
language = "English",
volume = "21",
pages = "125--129",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer",
number = "2",

}

In vitro germination and establishment of tissue cultures of Bulbine caulescens and of two Kniphofia species (Asphodelaceae). / Bringmann, G.; Noll, T.; Rischer, Heiko.

In: Plant Cell Reports, Vol. 21, No. 2, 2002, p. 125-129.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - In vitro germination and establishment of tissue cultures of Bulbine caulescens and of two Kniphofia species (Asphodelaceae)

AU - Bringmann, G.

AU - Noll, T.

AU - Rischer, Heiko

PY - 2002

Y1 - 2002

N2 - A protocol for the in vitro germination and the establishment of tissue cultures of Bulbine caulescens Linn., Kniphofia pumila Kunth, and K. uvaria Hook has been developed. The members of the family Asphodelaceae contain interesting substances with antiplasmodial activity against several strains of Plasmodium falciparum. With respect to species of Bulbine, this is the first time that in vitro germination has been achieved. For seeds of K. pumila and K. uvaria, a facilitated germination was attained by an optimized pretreatment of the seeds. After in vitro germination, root explants of Kniphofia and bulb explants of B. caulescens were successfully transformed into callus cultures. From these, liquid cultures of the two species of Kniphofia were easily produced. Studies on shoot multiplication of Kniphofia in vitro were also performed.

AB - A protocol for the in vitro germination and the establishment of tissue cultures of Bulbine caulescens Linn., Kniphofia pumila Kunth, and K. uvaria Hook has been developed. The members of the family Asphodelaceae contain interesting substances with antiplasmodial activity against several strains of Plasmodium falciparum. With respect to species of Bulbine, this is the first time that in vitro germination has been achieved. For seeds of K. pumila and K. uvaria, a facilitated germination was attained by an optimized pretreatment of the seeds. After in vitro germination, root explants of Kniphofia and bulb explants of B. caulescens were successfully transformed into callus cultures. From these, liquid cultures of the two species of Kniphofia were easily produced. Studies on shoot multiplication of Kniphofia in vitro were also performed.

KW - Bulbine caulescens; Kniphofia pumila; Kniphofia uvaria; In vitro germination

U2 - 10.1007/s00299-002-0497-1

DO - 10.1007/s00299-002-0497-1

M3 - Article

VL - 21

SP - 125

EP - 129

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 2

ER -