In Vitro Gut Metabolism of [U-13C]-Quinic Acid, The Other Hydrolysis Product of Chlorogenic Acid

Martine Naranjo Pinta, Ivan Montoliu, Anna Marja Aura, Tuulikki Seppänen-Laakso, Denis Barron, Sofia Moco (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    33 Citations (Scopus)


    Scope: Quinic acid in its free form is broadly abundant in plants, and can accumulate in copious amounts in coffee, tea, and certain fruits. However, it has been mostly studied as chlorogenic acid, an ester of caffeic and quinic acids. When chlorogenic acid reaches the colon, it is hydrolyzed by microbial esterases releasing caffeic and quinic acids. While biotransformation of chlorogenic and caffeic acids have been elucidated by in vitro and in vivo studies, the gut metabolism of quinic acid has been so far overlooked. Methods and Results: [U-13C]-Quinic acid is submitted to a colonic model using human fecal microbiota for assessing its metabolic fate. The metabolite profiles formed along microbial biotransformation are monitored by a combined metabolomics approach, using both 2D GC– and ultra-HPLC–MS. Six metabolic intermediates are identified by incorporation of isotopic label. Conclusion: Two parallel degradation pathways could be proposed: (1) an oxidative route, leading to aromatization and accumulation of protocatechuic acid, and a (2) reductive route, including dehydroxylation to cyclohexane carboxylic acid. Elucidating the biotransformation of food bioactives by the gut microbiota is of relevance for understanding nutrition, interindividual variability and potential effects on human metabolism.

    Original languageEnglish
    Article number1800396
    JournalMolecular Nutrition and Food Research
    Issue number22
    Publication statusPublished - 1 Nov 2018
    MoE publication typeA1 Journal article-refereed


    • colonic model
    • gut metabolism
    • metabolomics
    • quinate
    • quinic acid
    • stable isotopes


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