In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy

Ruixue Zhu, Tatiana Avsievich, Alexey Popov, Alexander Bykov, Igor Meglinski

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation.

Original languageEnglish
Title of host publicationOptical Trapping and Optical Micromanipulation XVIII
EditorsKishan Dholakia, Gabriel C. Spalding
PublisherInternational Society for Optics and Photonics SPIE
ISBN (Electronic)978-1-5106-4435-9
ISBN (Print)978-1-5106-4434-2
DOIs
Publication statusPublished - 2021
MoE publication typeA4 Article in a conference publication
EventOptical Trapping and Optical Micromanipulation XVIII 2021 - San Diego, United States
Duration: 1 Aug 20215 Aug 2021

Publication series

SeriesProceedings of SPIE - The International Society for Optical Engineering
Volume11798
ISSN0277-786X

Conference

ConferenceOptical Trapping and Optical Micromanipulation XVIII 2021
Country/TerritoryUnited States
CitySan Diego
Period1/08/215/08/21

Keywords

  • laser irradiation
  • light microscopy
  • optical tweezers
  • RBC aggregation
  • Red blood cells (RBCs)

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