In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy

Ruixue Zhu; Tatiana Avsievich; Alexey Popov; Alexander Bykov; Igor Meglinski

doi:10.1117/12.2595118

In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy

Ruixue Zhu^*, Tatiana Avsievich, Alexey Popov, Alexander Bykov, Igor Meglinski

^*Corresponding author for this work

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Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

Abstract

The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation.

Original language	English
Title of host publication	Optical Trapping and Optical Micromanipulation XVIII
Editors	Kishan Dholakia, Gabriel C. Spalding
Publisher	International Society for Optics and Photonics SPIE
ISBN (Electronic)	978-1-5106-4435-9
ISBN (Print)	978-1-5106-4434-2
DOIs	https://doi.org/10.1117/12.2595118
Publication status	Published - 2021
MoE publication type	A4 Article in a conference publication
Event	Optical Trapping and Optical Micromanipulation XVIII 2021 - San Diego, United States Duration: 1 Aug 2021 → 5 Aug 2021

Publication series

Series	Proceedings of SPIE
Volume	11798
ISSN	0277-786X

Conference

Conference	Optical Trapping and Optical Micromanipulation XVIII 2021
Country/Territory	United States
City	San Diego
Period	1/08/21 → 5/08/21

Funding

This work was partially funded by the China Scholarship Council (CSC No. 201706410089, R.Z.). R.Z. acknowledges X.Y. Su (School of Science, Beijing Jiaotong University, Beijing, China) for technical help in laser irradiation source selection. The authors also acknowledge the contribution of Russian Science Foundation (Projects: 19-72-30012 and 20-64-46003). I.M. acknowledges partial support from the Academy of Finland (project 325,097).

Keywords

laser irradiation
light microscopy
optical tweezers
RBC aggregation
Red blood cells (RBCs)

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10.1117/12.2595118

Cite this

Zhu, R., Avsievich, T., Popov, A., Bykov, A., & Meglinski, I. (2021). In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy. In K. Dholakia, & G. C. Spalding (Eds.), Optical Trapping and Optical Micromanipulation XVIII Article 1179809 International Society for Optics and Photonics SPIE. https://doi.org/10.1117/12.2595118

Zhu, Ruixue ; Avsievich, Tatiana ; Popov, Alexey et al. / In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy. Optical Trapping and Optical Micromanipulation XVIII. editor / Kishan Dholakia ; Gabriel C. Spalding. International Society for Optics and Photonics SPIE, 2021. (Proceedings of SPIE, Vol. 11798).

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title = "In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy",

abstract = "The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation. ",

keywords = "laser irradiation, light microscopy, optical tweezers, RBC aggregation, Red blood cells (RBCs)",

author = "Ruixue Zhu and Tatiana Avsievich and Alexey Popov and Alexander Bykov and Igor Meglinski",

note = "Funding Information: This work was partially funded by the China Scholarship Council (CSC No. 201706410089, R.Z.). R.Z. acknowledges X.Y. Su (School of Science, Beijing Jiaotong University, Beijing, China) for technical help in laser irradiation source selection. The authors also acknowledge the contribution of Russian Science Foundation (Projects: 19-72-30012 and 20-64-46003). I.M. acknowledges partial support from the Academy of Finland (project 325,097). ; Optical Trapping and Optical Micromanipulation XVIII 2021 ; Conference date: 01-08-2021 Through 05-08-2021",

year = "2021",

doi = "10.1117/12.2595118",

language = "English",

isbn = "978-1-5106-4434-2 ",

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Zhu, R, Avsievich, T, Popov, A, Bykov, A & Meglinski, I 2021, In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy. in K Dholakia & GC Spalding (eds), Optical Trapping and Optical Micromanipulation XVIII., 1179809, International Society for Optics and Photonics SPIE, Proceedings of SPIE, vol. 11798, Optical Trapping and Optical Micromanipulation XVIII 2021, San Diego, United States, 1/08/21. https://doi.org/10.1117/12.2595118

In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy. / Zhu, Ruixue; Avsievich, Tatiana; Popov, Alexey et al.
Optical Trapping and Optical Micromanipulation XVIII. ed. / Kishan Dholakia; Gabriel C. Spalding. International Society for Optics and Photonics SPIE, 2021. 1179809 (Proceedings of SPIE, Vol. 11798).

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

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AU - Popov, Alexey

AU - Bykov, Alexander

AU - Meglinski, Igor

N1 - Funding Information: This work was partially funded by the China Scholarship Council (CSC No. 201706410089, R.Z.). R.Z. acknowledges X.Y. Su (School of Science, Beijing Jiaotong University, Beijing, China) for technical help in laser irradiation source selection. The authors also acknowledge the contribution of Russian Science Foundation (Projects: 19-72-30012 and 20-64-46003). I.M. acknowledges partial support from the Academy of Finland (project 325,097).

PY - 2021

Y1 - 2021

N2 - The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation.

AB - The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation.

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ER -

Zhu R, Avsievich T, Popov A, Bykov A, Meglinski I. In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy. In Dholakia K, Spalding GC, editors, Optical Trapping and Optical Micromanipulation XVIII. International Society for Optics and Photonics SPIE. 2021. 1179809. (Proceedings of SPIE, Vol. 11798). doi: 10.1117/12.2595118

In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy

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