Abstract
The human gut hosts a microbial community which actively contributes to
the host metabolism and has thus remarkable effect on our health.
Intestinal microbiota is known to interact remarkably with the dietary
constituents entering the colon, causing major metabolic conversions
prior to absorption. To investigate the effect of microbial metabolism
on the phytochemical pool of rye bran, we applied an in vitro
simulated colonic fermentation where samples were collected with
intervals and analyzed by LC-MS based non-targeted metabolite profiling.
The analyses revealed extensive metabolic turnover on the phytochemical
composition of the bran samples, and showed effects on all the
metabolite classes detected. Furthermore, the majority of the
metabolites, both the precursors and the conversion products, remained
unidentified indicating that there are numerous yet unknown
phytochemicals, which can potentially affect on our health. This
underlines the importance of comprehensive profiling assays and
subsequent detailed molecular investigations in order to clarify the
effect of microbiota on phytochemicals present in our everyday diet.
Original language | English |
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Article number | e39322 |
Number of pages | 10 |
Journal | PLoS ONE |
Volume | 7 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2012 |
MoE publication type | A1 Journal article-refereed |