Abstract
| Original language | English |
|---|---|
| Article number | e39322 |
| Number of pages | 10 |
| Journal | PLoS ONE |
| Volume | 7 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2012 |
| MoE publication type | A1 Journal article-refereed |
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In vitro microbiotic fermentation causes an extensive metabolite turnover of rye bran phytochemicals. / Hanhineva, Kati (Corresponding Author); Aura, Anna-Marja; Rogachev, Ilana; Matero, Sanni; Skov, Thomas; Aharoni, Asaph; Poutanen, Kaisa; Mykkänen, Hannu.
In: PLoS ONE, Vol. 7, No. 6, e39322, 2012.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - In vitro microbiotic fermentation causes an extensive metabolite turnover of rye bran phytochemicals
AU - Hanhineva, Kati
AU - Aura, Anna-Marja
AU - Rogachev, Ilana
AU - Matero, Sanni
AU - Skov, Thomas
AU - Aharoni, Asaph
AU - Poutanen, Kaisa
AU - Mykkänen, Hannu
PY - 2012
Y1 - 2012
N2 - The human gut hosts a microbial community which actively contributes to the host metabolism and has thus remarkable effect on our health. Intestinal microbiota is known to interact remarkably with the dietary constituents entering the colon, causing major metabolic conversions prior to absorption. To investigate the effect of microbial metabolism on the phytochemical pool of rye bran, we applied an in vitro simulated colonic fermentation where samples were collected with intervals and analyzed by LC-MS based non-targeted metabolite profiling. The analyses revealed extensive metabolic turnover on the phytochemical composition of the bran samples, and showed effects on all the metabolite classes detected. Furthermore, the majority of the metabolites, both the precursors and the conversion products, remained unidentified indicating that there are numerous yet unknown phytochemicals, which can potentially affect on our health. This underlines the importance of comprehensive profiling assays and subsequent detailed molecular investigations in order to clarify the effect of microbiota on phytochemicals present in our everyday diet.
AB - The human gut hosts a microbial community which actively contributes to the host metabolism and has thus remarkable effect on our health. Intestinal microbiota is known to interact remarkably with the dietary constituents entering the colon, causing major metabolic conversions prior to absorption. To investigate the effect of microbial metabolism on the phytochemical pool of rye bran, we applied an in vitro simulated colonic fermentation where samples were collected with intervals and analyzed by LC-MS based non-targeted metabolite profiling. The analyses revealed extensive metabolic turnover on the phytochemical composition of the bran samples, and showed effects on all the metabolite classes detected. Furthermore, the majority of the metabolites, both the precursors and the conversion products, remained unidentified indicating that there are numerous yet unknown phytochemicals, which can potentially affect on our health. This underlines the importance of comprehensive profiling assays and subsequent detailed molecular investigations in order to clarify the effect of microbiota on phytochemicals present in our everyday diet.
U2 - 10.1371/journal.pone.0039322
DO - 10.1371/journal.pone.0039322
M3 - Article
VL - 7
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 6
M1 - e39322
ER -