Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae), a West African liana producing naphthylisoquinoline alkaloids, was successfully raised from seeds in vitro. Clonal propagation was best achieved growing nodal stem segments on 1/5 Linsmaier and Skoog medium with full strength organics and supplemented with 0.02 μM thidiazuron, 4.44 μM 6-benzylaminopurine and 0.05 μM 1-naphthaleneacetic acid. Detached axillary shoots were grown on Anderson's Rhododendron medium devoid of phytohormones and rooted within one month when dipped in 4.92 μM indole-3-butyric acid. Rooted plants became acclimatized to nonsterile greenhouse conditions.
Bringmann, G., Rischer, H., Schlauer, J., & Aké Assi, L. (1999). In vitro propagation of Ancistrocladus abbreviatus airy shaw (Ancistrocladaceae). Plant Cell, Tissue and Organ Culture, 57(1), 71-73. https://doi.org/10.1023/A:1006201711390