In vitro propagation of Ancistrocladus abbreviatus airy shaw (Ancistrocladaceae)

Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae), a West African liana producing naphthylisoquinoline alkaloids, was successfully raised from seeds in vitro. Clonal propagation was best achieved growing nodal stem segments on 1/5 Linsmaier and Skoog medium with full strength organics and supplemented with 0.02 µM thidiazuron, 4.44 µM 6-benzylaminopurine and 0.05 µM 1-naphthaleneacetic acid. Detached axillary shoots were grown on Anderson's Rhododendron medium devoid of phytohormones and rooted within one month when dipped in 4.92 µM indole-3-butyric acid. Rooted plants became acclimatized to nonsterile greenhouse conditions.