In Vitro Synthesis and Self-Assembly of Cellulose II Nanofibrils Catalyzed by the Reverse Reaction of Clostridium thermocellum Cellodextrin Phosphorylase

Robert Pylkkänen (Corresponding Author), Pezhman Mohammadi, Suvi Arola, Jorg C. de Ruijter, Naoki Sunagawa, Kiyohiko Igarashi (Corresponding Author), Merja Penttilä (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

Abstract

In nature, various organisms produce cellulose as microfibrils, which are processed into their nano- and microfibrillar and/or crystalline components by humans in order to obtain desired material properties. Interestingly, the natural synthesis machinery can be circumvented by enzymatically synthesizing cellulose from precursor molecules in vitro. This approach is appealing for producing tailor-made cellulosic particles and materials because it enables optimization of the reaction conditions for cellulose synthesis in order to generate particles with a desired morphology in their pure form. Here, we present enzymatic cellulose synthesis catalyzed by the reverse reaction of Clostridium thermocellum cellodextrin phosphorylase in vitro. We were able to produce cellulose II nanofibril networks in all conditions tested, using varying concentrations of the glycosyl acceptors d-glucose or d-cellobiose (0.5, 5, and 50 mM). We show that shorter cellulose chains assemble into flat ribbon-like fibrils with greater diameter, while longer chains assemble into cylindrical fibrils with smaller diameter.
Original languageUndefined
Pages (from-to)4355-4364
JournalBiomacromolecules
Volume21
Issue number10
DOIs
Publication statusPublished - 2020
MoE publication typeA1 Journal article-refereed

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